scholarly journals An EPR Study on the Interaction between the Cu(I) Metal Binding Domains of ATP7B and the Atox1 Metallochaperone

2020 ◽  
Vol 21 (15) ◽  
pp. 5536
Author(s):  
Michael Zaccak ◽  
Zena Qasem ◽  
Lada Gevorkyan-Airapetov ◽  
Sharon Ruthstein
Keyword(s):  
Metal Binding ◽  
Continuous Wave ◽  
Regulation System ◽  
Membrane Domain ◽  
Paramagnetic Resonance ◽  
Copper Trafficking ◽  
Binding Domains ◽  
Metal Binding Domain ◽  
Pulsed Electron Paramagnetic Resonance ◽  
Electron Paramagnetic

Copper’s essentiality and toxicity mean it requires a sophisticated regulation system for its acquisition, cellular distribution and excretion, which until now has remained elusive. Herein, we applied continuous wave (CW) and pulsed electron paramagnetic resonance (EPR) spectroscopy in solution to resolve the copper trafficking mechanism in humans, by considering the route travelled by Cu(I) from the metallochaperone Atox1 to the metal binding domains of ATP7B. Our study revealed that Cu(I) is most likely mediated by the binding of the Atox1 monomer to metal binding domain 1 (MBD1) and MBD4 of ATP7B in the final part of its extraction pathway, while the other MBDs mediate this interaction and participate in copper transfer between the various MBDs to the ATP7B membrane domain. This research also proposes that MBD1-3 and MBD4-6 act as two independent units.

scholarly journals Characterisation of the Fe(III):H+ Defect Centre in Natural Amethyst

2020 ◽  
Vol 51 (11) ◽  
pp. 1607-1619
Author(s):  
Francesco Di Benedetto ◽  
Alfonso Zoleo ◽  
Maurizio Romanelli
Keyword(s):  
Hyperfine Coupling ◽  
Continuous Wave ◽  
Spin Echo ◽  
Tetrahedral Site ◽  
Electron Spin Echo ◽  
Charge Balance ◽  
Paramagnetic Resonance ◽  
Isotropic Hyperfine Coupling ◽  
Pulsed Electron Paramagnetic Resonance ◽  
Electron Paramagnetic

AbstractA natural single crystal of amethyst was investigated by means of continuous-wave and pulsed Electron Paramagnetic Resonance (EPR), with the aim of structurally characterizing the substitutional S2 Fe(III):H+ centre. In this centre, Fe(III) replaces Si(IV) in the tetrahedral site, whereas H+ is coupled to Fe(III) to maintain the charge balance. The spectroscopic investigations, mainly the interpretation of the Electron Spin Echo Envelope Modulation, allowed a detailed localisation of the proton to be obtained. H+ occurs in the channels crossing the crystal parallel to the crystallographic c axis, in a largely eccentric position. The Fe(III)-H+ distance, evaluated in 2.70 Å, is found associated with a non-negligible isotropic hyperfine coupling, which can be linked to the relative stability of the S2 centre in natural amethyst.

Spin dynamics study in doped polyaniline by continuous wave and pulsed electron paramagnetic resonance

2000 ◽  
Vol 112 (6) ◽  
pp. 2958-2966 ◽  
Author(s):  
C. J. Magon ◽  
R. R. de Souza ◽  
A. J. Costa-Filho ◽  
E. A. Vidoto ◽  
R. M. Faria ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Continuous Wave ◽  
Spin Dynamics ◽  
Paramagnetic Resonance ◽  
Doped Polyaniline ◽  
Pulsed Electron Paramagnetic Resonance ◽  
Electron Paramagnetic

scholarly journals Coherent manipulation and quantum phase interference in a fullerene-based electron triplet molecular qutrit

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Ye-Xin Wang ◽  
Zheng Liu ◽  
Yu-Hui Fang ◽  
Shen Zhou ◽  
Shang-Da Jiang ◽  
...  
Keyword(s):  
Quantum Information Processing ◽  
Quantum Phase ◽  
Information Storage ◽  
Paramagnetic Resonance ◽  
Molecular Electron ◽  
Phase Interference ◽  
Superposition States ◽  
Pulsed Electron Paramagnetic Resonance ◽  
Electron Paramagnetic ◽  
Coherent Manipulation

AbstractHigh-spin magnetic molecules are promising candidates for quantum information processing because their intrinsic multiplicity facilitates information storage and computational operations. However, due to the absence of suitable sublevel splittings, their susceptibility to environmental disturbances and limitation from the selection rule, the arbitrary control of the quantum state of a molecular electron multiplet has not been realized. Here, we exploit the photoexcited triplet of C70 as a molecular electron spin qutrit with pulsed electron paramagnetic resonance. We prepared the system into 3-level superposition states characteristic of a qutrit and validated them by the tomography of their density matrices. To further elucidate the coherence of the operation and the nature of the system as a qutrit, we demonstrated the quantum phase interference in the superposition. The interference pattern is further interpreted as a map of possible evolution paths in the space of phase factors, representing the quantum nature of the 3-level system.

scholarly journals Probing the Hydrogen Bonding of the Ferrous–NO Heme Center of nNOS by Pulsed Electron Paramagnetic Resonance

2015 ◽  
Vol 119 (25) ◽  
pp. 6641-6649 ◽  
Author(s):  
Andrei V. Astashkin ◽  
Li Chen ◽  
Bradley O. Elmore ◽  
Deepak Kunwar ◽  
Yubin Miao ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Hydrogen Bonding ◽  
Paramagnetic Resonance ◽  
Pulsed Electron Paramagnetic Resonance ◽  
Electron Paramagnetic
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