scholarly journals Colistin Sulfate Chiral Stationary Phase for the Enantioselective Separation of Pharmaceuticals Using Organic Polymer Monolithic Capillary Chromatography

Molecules ◽  
2019 ◽  
Vol 24 (5) ◽  
pp. 833 ◽  
Author(s):  
Ali Fouad ◽  
Montaser Shaykoon ◽  
Samy Ibrahim ◽  
Sobhy El-Adl ◽  
Ashraf Ghanem
Keyword(s):  
Chiral Stationary Phase ◽  
Antiarrhythmic Drugs ◽  
Reversed Phase ◽  
Antifungal Drugs ◽  
Organic Polymer ◽  
Macrocyclic Antibiotic ◽  
Chiral Phases ◽  
Colistin Sulfate ◽  
Sedative Hypnotics ◽  
Inflammatory Drugs

A new functionalized polymer monolithic capillary with a macrocyclic antibiotic, namely colistin sulfate, as chiral selector was prepared via the copolymerization of binary monomer mixtures consisting of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA) in porogenic solvents namely 1-propanol and 1,4-butanediol, in the presence of azobisiso-butyronitrile (AIBN) as initiator and colistin sulfate. The prepared capillaries were investigated for the enantioselective nano-LC separation of a group of racemic pharmaceuticals, namely, α- and β-blockers, anti-inflammatory drugs, antifungal drugs, norepinephrine-dopamine reuptake inhibitors, catecholamines, sedative hypnotics, antihistaminics, anticancer drugs, and antiarrhythmic drugs. Acceptable separation was achieved for many drugs using reversed phase chromatographic conditions with no separation achieved under normal phase conditions. Colistin sulfate appears to be useful addition to the available macrocyclic antibiotic chiral phases used in liquid chromatography.

scholarly journals Daptomycin: A Novel Macrocyclic Antibiotic as a Chiral Selector in an Organic Polymer Monolithic Capillary for the Enantioselective Analysis of a Set of Pharmaceuticals

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3527
Author(s):  
Ali Fouad ◽  
Adel A. Marzouk ◽  
Montaser Sh. A. Shaykoon ◽  
Samy M. Ibrahim ◽  
Sobhy M. El-Adl ◽  
...  
Keyword(s):  
Glycidyl Methacrylate ◽  
Chiral Stationary Phase ◽  
Reversed Phase ◽  
Chiral Selector ◽  
Antifungal Drugs ◽  
Organic Polymer ◽  
Rp Hplc ◽  
Macrocyclic Antibiotic ◽  
Adrenergic Blockers ◽  
H1 Blockers

Daptomycin, a macrocyclic antibiotic, is here used as a new chiral selector in preparation of chiral stationary phase (CSP) in a recently prepared polymer monolithic capillary. The latter is prepared using the copolymerization of the monomers glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA) in the presence of daptomycin in water. Under reversed phase conditions (RP), the prepared capillaries were tested for the enantioselective nanoliquid chromatographic separation of fifty of the racemic drugs of different pharmacological groups, such as adrenergic blockers, H1-blockers, NSAIDs, antifungal drugs, and others. Baseline separation was attained for many drugs under RP-HPLC. Daptomycin expands the horizon of chiral selectors in HPLC.

scholarly journals Separation Of Methionine Enantiomers By Using Teicoplanin And Cyclofructan Columns

2015 ◽  
Vol 14 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Katarína Hroboňová ◽  
Zuzana Deáková ◽  
Jakub Moravčík ◽  
Jozef Lehotay ◽  
Daniel W. Armstrong ◽  
...  
Keyword(s):  
High Performance ◽  
Chiral Stationary Phase ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Enantiomeric Separation ◽  
Reversed Phase ◽  
Mobile Phase Composition ◽  
Dipole Interactions ◽  
Naturally Occurring ◽  
Macrocyclic Antibiotic

Abstract Methionine is a naturally occurring amino acid. Its enantiomeric separation by using high performance liquid chromatography on various types of chiral stationary phases was studied. The effect of mobile phase composition on enantioselectivity and retention was considered. The separation of the enantiomers was attained in different separation modes – reversed phase mode for the macrocyclic antibiotic chiral stationary phases (teicoplanin, teicoplanin aglycone), normal phase and polar organic phase modes for the isopropyl carbamate cyclofructan 6 chiral stationary phase. It was shown that the hydrogen bonding, dipole interactions, steric effects between methionine molecules and stationary phases play an important role in the separation of enantiomers.

Features of the adsorption of Naproxen on the chiral stationary phase (S,S)-Whelk-O1 under reversed-phase conditions

2010 ◽  
Vol 1217 (3) ◽  
pp. 264-275 ◽  
Author(s):  
Leonid Asnin ◽  
Fabrice Gritti ◽  
Krzysztof Kaczmarski ◽  
Georges Guiochon
Keyword(s):  
Stationary Phase ◽  
Chiral Stationary Phase ◽  
Reversed Phase

Stereoselective HPLC analysis of tertatolol in rat plasma using macrocyclic antibiotic chiral stationary phase

Chirality ◽  
2010 ◽  
Vol 23 (4) ◽  
pp. 333-338 ◽  
Author(s):  
Mohamed M. Hefnawy ◽  
Yousif A. Asiri ◽  
Nourah Z. Al-Zoman ◽  
Gamal. A. Mostafa ◽  
Hassan Y. Aboul-Enein
Keyword(s):  
Stationary Phase ◽  
Hplc Analysis ◽  
Chiral Stationary Phase ◽  
Rat Plasma ◽  
Macrocyclic Antibiotic

Simultaneous liquid-chromatographic determination of three antiarrhythmic drugs: disopyramide, lidocaine, and quinidine.

1980 ◽  
Vol 26 (2) ◽  
pp. 197-200
Author(s):  
J G Flood ◽  
G N Bowers ◽  
R B McComb
Keyword(s):  
Mobile Phase ◽  
Antiarrhythmic Drugs ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Peak Height ◽  
Reversed Phase ◽  
Multiple Drug ◽  
Evaporation Technique ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract We report a common methodology for determining three antiarrhythmic drugs: disopyramide, lidocaine, and quinidine. Alkalinized serum and internal standard (p-chlorodisopyramide) are extracted into dichloromethane, the organic phase is evaporated, and the redissolved residue is injected onto a reversed-phase column (micron Bondapack C18). Quantitation is via peak-height ratios of analyte vs internal standard (as detected at 205 nm) referenced to a serum-based multiple-drug standard. A mobile phase of 30 mmol/L phosphate buffer and acetonitrile (72/28 by vol) is used. These conditions yiel; optimum separation and band symmetry for the analytes and some of their metabolites. Crucial factors in this simultaneous assay include pH of the mobile phase and injected solution, extraction time, and evaporation technique. Day-to-day precision (CV) for all drugs was less than 5%, and correlation with other assay techniques for each drug is reported. The method enables more efficient use of personnel and instrumentation without sacrificing analytical quality.

scholarly journals Simultaneous liquid-chromatographic determination of three antiarrhythmic drugs: disopyramide, lidocaine, and quinidine.

1980 ◽  
Vol 26 (2) ◽  
pp. 197-200 ◽  
Author(s):  
J G Flood ◽  
G N Bowers ◽  
R B McComb
Keyword(s):  
Mobile Phase ◽  
Antiarrhythmic Drugs ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Peak Height ◽  
Reversed Phase ◽  
Multiple Drug ◽  
Evaporation Technique ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract We report a common methodology for determining three antiarrhythmic drugs: disopyramide, lidocaine, and quinidine. Alkalinized serum and internal standard (p-chlorodisopyramide) are extracted into dichloromethane, the organic phase is evaporated, and the redissolved residue is injected onto a reversed-phase column (micron Bondapack C18). Quantitation is via peak-height ratios of analyte vs internal standard (as detected at 205 nm) referenced to a serum-based multiple-drug standard. A mobile phase of 30 mmol/L phosphate buffer and acetonitrile (72/28 by vol) is used. These conditions yiel; optimum separation and band symmetry for the analytes and some of their metabolites. Crucial factors in this simultaneous assay include pH of the mobile phase and injected solution, extraction time, and evaporation technique. Day-to-day precision (CV) for all drugs was less than 5%, and correlation with other assay techniques for each drug is reported. The method enables more efficient use of personnel and instrumentation without sacrificing analytical quality.

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