scholarly journals Novel In Vivo Mouse Cryoablation Model to Explore Unique Therapeutic Approaches for Premalignant Columnar Lesions

2021 ◽  
Vol 4 (1) ◽  
pp. 6
Author(s):  
Ana C. P. Correia ◽  
Danielle Straub ◽  
Silvia Calpe ◽  
Kausilia K. Krishnadath

Patients with epithelial metaplasias have an increased risk of developing malignancies. In Barrett’s esophagus, neo-columnar epithelium develops proximal to the squamous-columnar junction (SCJ) in the esophagus as the result of prolonged exposure to bile and acid reflux. Patients require lifetime periodic surveillance, due to lack of effective eradication therapies. The shortage of innovative treatment options is mostly attributable to the paucity of adequate in vivo models of neo-columnar epithelium regeneration. This protocol describes the generation of a cryoablation model to study regeneration of neo-epithelia at the SCJ. Cryoablation of the columnar and squamous mucosa at the SCJ was achieved through local application of liquid N2O in wild-type and reporter mice in combination with acid suppression. Acid suppression alone, showed restoration of the SCJ with normal histological features of both the neo-columnar and neo-squamous epithelium within 14 days. As a proof of principle, mice were treated with mNoggin, an inhibitor of bone morphogenetic proteins (BMPs), which are involved in the development of columnar epithelia. Local application of mNoggin to the ablated area at the SCJ significantly reduced the development of the neo-columnar mucosa. Although this model does not faithfully recapitulate the exact characteristics of Barrett’s esophagus, it is a well-suited tool to study the mechanisms of therapeutic inhibition of neo-columnar regeneration. It therefore represents an efficient and easy platform to test novel pharmacological therapies for treatment of neo-epithelial lesions at the SCJ.

2011 ◽  
Vol 140 (5) ◽  
pp. S-75 ◽  
Author(s):  
Cathrine J. DeMars ◽  
Navtej Buttar

2021 ◽  
Author(s):  
Luka Vranić ◽  
Tin Nadarević ◽  
Davor Štimac

Background: Barrett’s esophagus (BE) requires surveillance to identify potential neoplasia at early stage. Standard surveillance regimen includes random four-quadrant biopsies by Seattle protocol. Main limitations of random biopsies are high risk of sampling error, difficulties in histology interpretation, common inadequate classification of pathohistological changes, increased risk of bleeding and time necessary to acquire the final diagnosis. Probe-based confocal laser endomicroscopy (pCLE) has emerged as a potential tool with an aim to overcome these obvious limitations. Summary: pCLE represents real-time microscopic imaging method that offers evaluation of epithelial and subepithelial structures with 1000-fold magnification. In theory, pCLE has potential to eliminate the need for biopsy in BE patient. The main advantages would be real-time diagnosis and decision making, greater diagnostic accuracy and to evaluate larger area compared to random biopsies. Clinical pCLE studies in esophagus show high diagnostic accuracy and its high negative predictive value offers high reliability and confidence to exclude dysplastic and neoplastic lesions. However, it still cannot replace histopathology due to lower positive predictive value and sensitivity. Key messages: Despite promising results, its role in routine use in patients with Barrett’s esophagus remains questionable primarily due to lack of well-organized double-blind randomized trials.


2019 ◽  
Vol 18 (3) ◽  
pp. 425-429 ◽  
Author(s):  
Rita M. Knotts ◽  
Quinn S. Solfisburg ◽  
Claire Keating ◽  
Emily DiMango ◽  
Charles J. Lightdale ◽  
...  

Endoscopy ◽  
2001 ◽  
Vol 33 (5) ◽  
pp. 391-400 ◽  
Author(s):  
M. I. Canto ◽  
S. Setrakian ◽  
J. E. Willis ◽  
A. Chak ◽  
R. E. Petras ◽  
...  

Digestion ◽  
2004 ◽  
Vol 70 (2) ◽  
pp. 95-102 ◽  
Author(s):  
Hitoshi Nishigaki ◽  
Ken Wada ◽  
Atsushi Tatsuguchi ◽  
Nobuo Sueoka ◽  
Seiji Futagami ◽  
...  

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