scholarly journals Evaluation of Silica-Coated Insect Proof Nets for the Control of Aphis fabae, Sitophilus oryzae, and Tribolium confusum

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1658
Author(s):  
Paraskevi Agrafioti ◽  
Sofia Faliagka ◽  
Evagelia Lampiri ◽  
Merle Orth ◽  
Mark Pätzel ◽  
...  

Insect proof nets are widely used in agriculture as mechanical and physical barriers to regulate pest populations in a greenhouse. However, their integration in the greenhouse ventilation openings is highly associated with the decrease of air flow and the adequate ventilation. Thus, there is need for alternative pest management tools that do not impair adequate ventilation. In the present study, we tested four net formulations of relatively large mesh size coated with SiO2 nanoparticles, namely, ED3, ED3-P, ED5, and ED5-P to evaluate their insecticidal properties against adults of Aphis fabae and Sitophilus oryzae and larvae of Tribolium confusum. ED3 and ED5 nets were coated with SiO2 nanoparticles of different diameter, while in the case of ED3-P and ED5-P, paraffin was added to increase the mass of the deposited particles on the net’s surface. In the first series of bioassays, the knockdown and mortality rates of these species were evaluated after exposure to the aforementioned net formulations for 5, 10, 15, 20, 25, 30, 60, 90, and 180 min. In the second series of bioassays, knockdown and mortality of these species were recorded after 1, 7, and 10 days of post-exposure to the nets for different time intervals (15, 30, and 60 min). Based on our results, all nets significantly affected A. fabae, since all insects were dead at the 1-day post-exposure period to the silica-treated nets. Conversely, at the same interval, no effect on either S. oryzae adults or T. confusum larvae was observed. However, in the case of S. oryzae, the efficacy of all nets reached 100% 7 days after the exposure, even for adults that had been initially exposed for 15 min to the treated nets. Among the species tested, T. confusum larvae exhibited the lowest mortality rate, which did not exceed 34% at the 10 days of post-exposure interval. Our work underlines the efficacy of treated nets in pest management programs, under different application scenarios, at the pre- and post-harvest stages of agricultural commodities.

2020 ◽  
Vol 137 ◽  
pp. 105312 ◽  
Author(s):  
Sofia Faliagka ◽  
Paraskevi Agrafioti ◽  
Evagelia Lampiri ◽  
Nikolaos Katsoulas ◽  
Christos G. Athanassiou

Parasite ◽  
2019 ◽  
Vol 26 ◽  
pp. 63
Author(s):  
Nadège Perier ◽  
Wilfried Lebon ◽  
Leon Meyer ◽  
Noua Lekouch ◽  
Nesrine Aouiche ◽  
...  

Twelve healthy dogs were included in this laboratory efficacy study. Six dogs were randomly allocated based on body weight to an untreated control group and six to an afoxolaner (NexGard®) treated group. In the treatment group, afoxolaner was administered orally on Day 0 in accordance with label instructions. On Days 1, 14 and 28, each dog was exposed to 60 unfed female and 10 male Phlebotomus perniciosus sandflies for 1 h. At the end of each exposure period, sandflies were counted and assessed for viability and feeding status. There was no statistical difference in mortality (0.0–5.4%), nor in feeding proportion (61.6–78%) between the control and the treated groups at all 1-h post-exposure assessments. After collection, live fed and unfed sandflies were kept for viability assessments at 48 and 72 h post-exposure. In the untreated control group, the average percentages of live, fed, female sandflies after exposure, on Days 1, 14 and 28, ranged from 51% to 74% at 48 h and from 46% to 57% at 72 h, demonstrating model robustness over the 28 days of the study. Significantly fewer live fed sandflies were recorded for the afoxolaner treated group (p < 0.01). The insecticidal efficacy was 100%, 95.9% and 75.2% at 48 h post Days 1, 14 and 28 exposures, respectively, and 100%, 100% and 86.3% at 72 h post Days 1, 14, and 28 exposures, respectively. A single administration of oral afoxolaner (NexGard®) to dogs significantly killed P. perniciosus sandflies 48 and 72 h after blood feeding for 1 month.


2014 ◽  
Vol 77 (1) ◽  
pp. 87-93 ◽  
Author(s):  
NICKOLAS G. KAVALLIERATOS ◽  
CHRISTOS G. ATHANASSIOU ◽  
MARIA M. AOUNTALA ◽  
DEMETRIUS C. KONTODIMAS

The entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea were tested against the stored-grain pest Sitophilus oryzae. The fungi were isolated from the soil (from three locations in Attica, Greece: B. bassiana from Tatoion, M. anisopliae from Marathon, and I. fumosorosea from Aghios Stefanos) using larvae of Galleria mellonella as bait. Suspensions of 2.11 × 107 and 2.11 × 108, 1.77 × 107 and 1.77 × 108, and 1.81 × 107 and 1.81 × 108 conidia per ml of B. bassiana, M. anisopliae, and I. fumosorosea, respectively, were applied by three treatments: (i) sprayed on food and set in petri dishes with adults of S. oryzae, (ii) sprayed on adults of S. oryzae and set in petri dishes without food, and (iii) sprayed on adults of S. oryzae and set in petri dishes with food. The observed mortality of S. oryzae adults during the overall exposure period for the lowest, as well as for the highest, concentrations of B. bassiana, M. anisopliae, and I. fumosorosea ranged from 0 to 100%. Concentration was, in most of the cases tested, a critical parameter that determined the “speed of kill” of the exposed insect species for B. bassiana and M. anisopliae. Conversely, concentration was not that critical for I. fumosorosea, and survival was high in some of the combinations tested, even after 14 days of exposure. Both in the highest and the lowest concentrations of fungi, the mortality of S. oryzae adults was higher when the fungi were applied on adults than when they were applied on food. Higher mortality was observed when food was absent than when food was present, in most of the cases tested. The high efficacy levels recorded in the current study indicate that the tested fungi could be effective biocontrol agents against S. oryzae.


Toxicology ◽  
2014 ◽  
Vol 316 ◽  
pp. 75
Author(s):  
Santos Dinamene ◽  
Batoreu M. Camila ◽  
I. Tavares de Almeida ◽  
L. Davis Randall ◽  
Mateus M. Luisa ◽  
...  

2019 ◽  
Vol 95 (12) ◽  
pp. 1138-1141 ◽  
Author(s):  
Galina M. Bodienkova ◽  
V. S. Rukavishnikov ◽  
E. V. Boklazhenko

Author(s):  
D.P. Locatelli ◽  
P. Girgenti ◽  
L. Moncini ◽  
L. Limonta

In this paper, the effect of a highly purified N2 (98.5%) controlled atmosphere-generated in situ by a membrane nitrogen separator on mixed age eggs of Sitophilus oryzae and Tribolium confusum was evaluated. Trials were carried out in an airtight stainless silo filled with wheat, in which a nitrogen atmosphere was maintained for durations lasting from one to several days. The mean temperature recorded during the treatment varied from 22 to 24°C. After the treatment, the eggs were transferred to a jar with rearing medium and kept at 27±1°C and 70±5% R.H. The jars were checked every two days to record adult emergence. Eighty percent mortality was observed in T. confusum and S. oryzae with two and four days of treatment, respectively. One hundred percent mortality was obtained after five days of treatment in the case of T. confusum and six days for S. oryzae.


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