scholarly journals Proteopeptidomic, Functional and Immunoreactivity Characterization of Bothrops moojeni Snake Venom: Influence of Snake Gender on Venom Composition

Toxins ◽  
2018 ◽  
Vol 10 (5) ◽  
pp. 177 ◽  
Author(s):  
Fernanda Amorim ◽  
Tassia Costa ◽  
Dominique Baiwir ◽  
Edwin De Pauw ◽  
Loic Quinton ◽  
...  
Keyword(s):  
Snake Venom ◽  
Venom Composition ◽  
Bothrops Moojeni

Characterization Of A Hemagglutinating Glycoprotein Isolated From Bothrops Moojeni Snake Venom

2001 ◽  
Vol 8 (1) ◽  
pp. 13-20 ◽  
Author(s):  
B. Kassab ◽  
D. de Carvalho ◽  
S. Marangoni ◽  
J. Novello
Keyword(s):  
Snake Venom ◽  
Bothrops Moojeni

Isolation and characterization of two new Lys49 PLA2s with heparin neutralizing properties from Bothrops moojeni snake venom

Toxicon ◽  
2010 ◽  
Vol 55 (6) ◽  
pp. 1080-1092 ◽  
Author(s):  
Anna Maria Perchuc ◽  
Laure Menin ◽  
Philippe Favreau ◽  
Beatrice Bühler ◽  
Philippe Bulet ◽  
...  
Keyword(s):  
Snake Venom ◽  
Isolation And Characterization ◽  
Bothrops Moojeni

Preliminary Biochemical and Venomic Characterization of the Venom of Phalotris lemniscatus (Serpentes, Colubridae)

2019 ◽  
Vol 19 (22) ◽  
pp. 1981-1989 ◽  
Author(s):  
Jeny Bastida ◽  
Alejandro Crampet ◽  
Melitta Meneghel ◽  
Victor Morais
Keyword(s):  
Amino Acid ◽  
Blood Coagulation ◽  
Snake Venom ◽  
Clinical Relevance ◽  
Protein Families ◽  
Snake Venoms ◽  
Highly Active ◽  
Venom Composition ◽  
Kunitz Type

Background: For many decades, research on snake venom toxinology focused mainly on the venoms of Viperidae and Elapidae species, which were traditionally the only ones considered as venomous. However, much less interest has been given to the venom produced by opisthoglyphous colubrid snakes, since they were typically considered of no clinical relevance. Objective: The aim of this work is to perform a preliminary biochemical and venomic characterization of the venom of the colubrid snake Phalotris lemniscatus, a species that has been responsible for two relevant cases of envenomation in Uruguay. Methods: We extracted venom from collected specimens and performed different biochemical and proteomic assays to understand its toxin composition. Results: We found that the venom of P. lemniscatus is composed of protein families typically present in snake venoms, such as metallo and serine preoteases, L-amino acid oxidases, phospholipases A2s, Ctype lectines-like, Kunitz-type proteins and three-finger toxins. Activity assays demonstrated a highly active gelatinolytic component as well as a potent capability to induce blood coagulation. Conclusion: The results indicate that the venom of P. lemniscatus contains hemotoxic activities and components that resemble those found in Viperidae (Bothrops) snakes and that can induce a clinically relevant accident. Further studies are needed to better understand the venom composition of this colubrid snake and its most active compounds.

scholarly journals Biochemical and functional characterization of BmooSP, a new serine protease from Bothrops moojeni snake venom

Toxicon ◽  
2016 ◽  
Vol 111 ◽  
pp. 130-138 ◽  
Author(s):  
Fábio de Oliveira ◽  
Bruna Barbosa de Sousa ◽  
Carla Cristine Neves Mamede ◽  
Nadia Cristina Gomes de Morais ◽  
Mayara Ribeiro de Queiroz ◽  
...  
Keyword(s):  
Serine Protease ◽  
Snake Venom ◽  
Functional Characterization ◽  
Bothrops Moojeni

Isolation and structural characterization of a new fibrin(ogen)olytic metalloproteinase from Bothrops moojeni snake venom

Toxicon ◽  
2008 ◽  
Vol 51 (4) ◽  
pp. 574-584 ◽  
Author(s):  
Carolina P. Bernardes ◽  
Norival A. Santos-Filho ◽  
Tássia R. Costa ◽  
Mário S.R. Gomes ◽  
Fernanda S. Torres ◽  
...  
Keyword(s):  
Snake Venom ◽  
Structural Characterization ◽  
Bothrops Moojeni

Structural and Functional Characterization of Myotoxin I, a Lys49 Phospholipase A2 Homologue from Bothrops moojeni (Caissaca) Snake Venom

2000 ◽  
Vol 373 (1) ◽  
pp. 7-15 ◽  
Author(s):  
Andreimar M Soares ◽  
Sı́lvia H Andrião-Escarso ◽  
Yamileth Angulo ◽  
Bruno Lomonte ◽  
José M Gutiérrez ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Snake Venom ◽  
Functional Characterization ◽  
Bothrops Moojeni

Biological and biochemical characterization of new basic phospholipase A2 BmTX-I isolated from Bothrops moojeni snake venom

Toxicon ◽  
2008 ◽  
Vol 51 (8) ◽  
pp. 1509-1519 ◽  
Author(s):  
Andrana K. Calgarotto ◽  
Daniela C.S. Damico ◽  
L.A. Ponce-Soto ◽  
Paulo A. Baldasso ◽  
Saulo L. Da Silva ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Snake Venom ◽  
Biochemical Characterization ◽  
Bothrops Moojeni

scholarly journals The genetics of venom ontogeny in the eastern diamondback rattlesnake (Crotalus adamanteus)

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3249 ◽  
Author(s):  
Darin R. Rokyta ◽  
Mark J. Margres ◽  
Micaiah J. Ward ◽  
Elda E. Sanchez
Keyword(s):  
Snake Venom ◽  
Venom Gland ◽  
Ontogenetic Changes ◽  
Intraspecific Divergence ◽  
Full Characterization ◽  
Venom Composition ◽  
Significant Difference ◽  
Diamondback Rattlesnake ◽  
Putative Toxin

The same selective forces that give rise to rapid inter- and intraspecific divergence in snake venoms can also favor differences in venoms across life-history stages. Ontogenetic changes in venom composition are well known and widespread in snakes but have not been investigated to the level of unambiguously identifying the specific loci involved. The eastern diamondback rattlesnake was previously shown to undergo an ontogenetic shift in venom composition at sexual maturity, and this shift accounted for more venom variation than geography. To characterize the genetics underlying the ontogenetic venom compositional change in C. adamanteus, we sequenced adult/juvenile pairs of venom-gland transcriptomes from five populations previously shown to have different adult venom compositions. We identified a total of 59 putative toxin transcripts for C. adamanteus, and 12 of these were involved in the ontogenetic change. Three toxins were downregulated, and nine were upregulated in adults relative to juveniles. Adults and juveniles expressed similar total levels of snake-venom metalloproteinases but differed substantially in their featured paralogs, and adults expressed higher levels of Bradykinin-potentiating and C-type natriuretic peptides, nerve growth factor, and specific paralogs of phospholipases A2 and snake venom serine proteinases. Juvenile venom was more toxic to mice, indicating that the expression differences resulted in a phenotypically, and therefore potentially ecologically, significant difference in venom function. We also showed that adult and juvenile venom-gland transcriptomes for a species with known ontogenetic venom variation were equally effective at individually providing a full characterization of the venom genes of a species but that any particular individual was likely to lack several toxins in their transcriptome. A full characterization of a species’ venom-gene complement therefore requires sequencing more than one individual, although the ages of the individuals are unimportant.

Characterization of Venoms of Deinagkistrodon acutus and Bungarus multicinctus Using Proteomics and Peptidomics

2020 ◽  
Vol 17 (3) ◽  
pp. 241-254
Author(s):  
Yaqiong Zhang ◽  
Zhiping Jia ◽  
Yunyang Liu ◽  
Xinwen Zhou ◽  
Yi Kong
Keyword(s):  
Snake Venom ◽  
Protein Families ◽  
Traditional Medicines ◽  
Phospholipases A2 ◽  
Inhibitory Peptides ◽  
Sds Page ◽  
Deinagkistrodon Acutus ◽  
Venom Proteins ◽  
Proteins And Peptides

Background: Deinagkistrodon acutus (D. acutus) and Bungarus multicinctus (B. multicinctus) as traditional medicines have been used for hundreds of years in China. The venoms of these two species have strong toxicity on the victims. Objective: The objective of this study is to reveal the profile of venom proteins and peptides of D. acutus and B. multicinctus. Method: Ultrafiltration, SDS-PAGE coupled with in-gel tryptic digestion and Liquid Chromatography- Electrospray Ionization-Tandem Mass Spectrometry (LC-ESI-MS/MS) were used to characterize proteins and peptides of venoms of D. acutus and B. multicinctus. Results: In the D. acutus venom, 67 proteins (16 protein families) were identified, and snake venom metalloproteinases (SVMPs, 38.0%) and snake venom C-type lectins (snaclecs, 36.7%) were dominated proteins. In the B. multicinctus venom, 47 proteins (15 protein families) were identified, and three-finger toxins (3FTxs, 36.3%) and Kunitz-type Serine Protease Inhibitors (KSPIs, 32.8%) were major components. In addition, both venoms contained small amounts of other proteins, such as Snake Venom Serine Proteinases (SVSPs), Phospholipases A2 (PLA2s), Cysteine-Rich Secreted Proteins (CRISPs), 5'nucleotidases (5'NUCs), Phospholipases B (PLBs), Phosphodiesterases (PDEs), Phospholipase A2 Inhibitors (PLIs), Dipeptidyl Peptidases IV (DPP IVs), L-amino Acid Oxidases (LAAOs) and Angiotensin-Converting Enzymes (ACEs). Each venom also had its unique proteins, Nerve Growth Factors (NGFs) and Hyaluronidases (HYs) in D. acutus, and Cobra Venom Factors (CVFs) in B. multicinctus. In the peptidomics, 1543 and 250 peptides were identified in the venoms of D. acutus and B. multicinctus, respectively. Some peptides showed high similarity with neuropeptides, ACE inhibitory peptides, Bradykinin- Potentiating Peptides (BPPs), LAAOs and movement related peptides. Conclusion: Characterization of venom proteins and peptides of D. acutus and B. multicinctus will be helpful for the treatment of envenomation and drug discovery.

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