scholarly journals Development and Evaluation of an Immuno-MALDI-TOF Mass Spectrometry Approach for Quantification of the Abrin Toxin in Complex Food Matrices

Toxins ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 52
Author(s):  
Sandrine Livet ◽  
Sylvia Worbs ◽  
Hervé Volland ◽  
Stéphanie Simon ◽  
Martin B. Dorner ◽  
...  

The toxin abrin found in the seeds of Abrus precatorius has attracted much attention regarding criminal and terroristic misuse over the past decade. Progress in analytical methods for a rapid and unambiguous identification of low abrin concentrations in complex matrices is essential. Here, we report on the development and evaluation of a MALDI-TOF mass spectrometry approach for the fast, sensitive and robust abrin isolectin identification, differentiation and quantification in complex food matrices. The method combines immunoaffinity-enrichment with specific abrin antibodies, accelerated trypsin digestion and the subsequent MALDI-TOF analysis of abrin peptides using labeled peptides for quantification purposes. Following the optimization of the workflow, common and isoform-specific peptides were detected resulting in a ~38% sequence coverage of abrin when testing ng-amounts of the toxin. The lower limit of detection was established at 40 ng/mL in milk and apple juice. Isotope-labeled versions of abundant peptides with high ionization efficiency were added. The quantitative evaluation demonstrated an assay variability at or below 22% with a linear range up to 800 ng/mL. MALDI-TOF mass spectrometry allows for a simple and fast (<5 min) analysis of abrin peptides, without a time-consuming peptide chromatographic separation, thus constituting a relevant alternative to liquid chromatography-tandem mass spectrometry.

2021 ◽  
pp. 103835
Author(s):  
Sébastien Bridel ◽  
Stephen C. Watts ◽  
Louise M. Judd ◽  
Taylor Harshegyi ◽  
Virginie Passet ◽  
...  

2001 ◽  
Vol 34 (7) ◽  
pp. 2389-2393 ◽  
Author(s):  
Weihua Ming ◽  
Xianwen Lou ◽  
Robert D. van de Grampel ◽  
Joost L. J. van Dongen ◽  
Rob van der Linde

2014 ◽  
Vol 226 (02) ◽  
pp. 68-71 ◽  
Author(s):  
M. Artacho-Reinoso ◽  
P. Olbrich ◽  
P. Solano-Paéz ◽  
P. Ybot-Gonzalez ◽  
J. Lepe ◽  
...  

2016 ◽  
Vol 120 ◽  
pp. 68-71 ◽  
Author(s):  
Gerald Larrouy-Maumus ◽  
Abigail Clements ◽  
Alain Filloux ◽  
Ronan R. McCarthy ◽  
Serge Mostowy

2010 ◽  
Vol 6 (11) ◽  
pp. 2214 ◽  
Author(s):  
Eleonora Cerasoli ◽  
Paulina D. Rakowska ◽  
Adrian Horgan ◽  
Jascindra Ravi ◽  
Melanie Bradley ◽  
...  

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