scholarly journals Some aspects of the genomic DNA isolation from lavender plants of different origin.

Author(s):  
M.S. Zagorskaya ◽  

The method of DNA extraction from lavender plants has some difficulties. Moreover, molecular genetic studies often use not only fresh materials but also microplants from an in vitro culture or frozen material. For all these types of samples, the DNA isolation technique was developed, which is based on the CTAB buffer, but without the use of liquid nitrogen, mercaptoethanol and sodium acetate, which, in turn, simplifies and reduces the cost of the method.

2015 ◽  
pp. 42-42
Author(s):  
Arti Pandey ◽  
Arun Pandey ◽  
Naveen Shreevastava ◽  
Durga Neupane

2016 ◽  
Vol 1 (1) ◽  
pp. 15-27 ◽  
Author(s):  
Kristen A. Leach ◽  
Paula C. McSteen ◽  
David M. Braun

2017 ◽  
Vol 8 (4) ◽  
pp. 045015 ◽  
Author(s):  
Gunajit Goswami ◽  
Himangshu Boruah ◽  
Trishnamoni Gautom ◽  
Dibya Jyoti Hazarika ◽  
Madhumita Barooah ◽  
...  

2013 ◽  
Vol 22 (4) ◽  
pp. 492-497 ◽  
Author(s):  
Lingling Zhang ◽  
Lijing Dai ◽  
Junbo Gou ◽  
Junhua Peng

2008 ◽  
Vol 63 (7-8) ◽  
pp. 595-598 ◽  
Author(s):  
Emel Sözen ◽  
Ismail Poyraz

Origanum onites is an economically important medicinal plant with high essential oil content. Lack of an appropriate DNA isolation procedure is a limiting factor for any molecular study of this plant. We have used a protocol for genomic DNA isolation based on a hexadecyltrimethylammonium bromide (CTAB) method described for other plant species. The method involves mortar grinding of leaf tissue, modified CTAB extraction using high salt concentrations and polyvinyl pyrrolidone, and successive isoamyl alcohol/chloroform extractions. The yield was approx. 20 μg DNA per 200 mg of initial fresh plant material. The genomic DNA obtained by this method was suitable to be used in restriction digests, inter simple sequence repeat (ISSR) and randomly amplified polymorphic DNA (RAPD) reactions. This extraction method should facilitate the molecular analysis of Origanum chemotypes.


PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e109061 ◽  
Author(s):  
Helen E. Barnes ◽  
Guohong Liu ◽  
Christopher Q. Weston ◽  
Paula King ◽  
Long K. Pham ◽  
...  

2005 ◽  
Vol 1064 (2) ◽  
pp. 247-253 ◽  
Author(s):  
Jana Křížová ◽  
Alena Španová ◽  
Bohuslav Rittich ◽  
Daniel Horák

Sign in / Sign up

Export Citation Format

Share Document