Animal feeding stuffs. Determination of selenium by hydride generation atomic absorption spectrometry (HGAAS) after microwave digestion (digestion with 65 % nitric acid and 30 % hydrogen peroxide)

2015 ◽  
Keyword(s):  
Hydrogen Peroxide ◽  
Nitric Acid ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Microwave Digestion ◽  
Hydride Generation ◽  
Absorption Spectrometry ◽  
Animal Feeding

scholarly journals Determination of Selenium in Urine by Hydride Generation Atomic Absorption Spectrometry

1996 ◽  
Vol 79 (3) ◽  
pp. 773-776 ◽  
Author(s):  
Miguel Navarro ◽  
Herminia Lopez ◽  
Maria C Lopez ◽  
Vidal Perez
Keyword(s):  
Nitric Acid ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Hydride Generation ◽  
Absorption Spectrometry ◽  
Acid Mixture ◽  
Acid Digestion ◽  
Daily Diet ◽  
Total Selenium

Abstract A procedure has been developed for determination of total selenium in urine by hydride generation atomic absorption spectrometry. Mineralization was performed with a nitric acid–perchloric acid mixture on a thermostated digestion block. The method was validated by comparison with the method involving mineralization in a microwave acid digestion bomb containing nitric acid and small amounts of vanadium pentoxide. Se(VI) was reduced to Se(IV) by dissolution in 7N HCI. Sample recoveries, precision studies, and analyses of a certified reference material demonstrated the reliability and accuracy of this technique. Urine samples had selenium concentrations ranging from 4.6 to 50.3 μg/L. These values correspond to an average of 54.9 μg per person per day total ingested and bioavailable Se in the daily diet.

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