Faculty Opinions recommendation of The effect of dissolved oxygen (DO) concentration on the glycosylation of recombinant protein produced by the insect cell-baculovirus expression system.

2002 ◽  
Author(s):  
Jeffrey Schwartz
Keyword(s):  
Recombinant Protein ◽  
Dissolved Oxygen ◽  
Insect Cell ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Baculovirus Expression ◽  
Do Concentration

Production of therapeutic proteins with baculovirus expression system in insect cell

2008 ◽  
Vol 38 ◽  
pp. S71-S78 ◽  
Author(s):  
Mi-Hyun AHN ◽  
Mira SONG ◽  
Eun-Yi OH ◽  
Arshad JAMAL ◽  
HyunSoon KIM ◽  
...  
Keyword(s):  
Insect Cell ◽  
Expression System ◽  
Therapeutic Proteins ◽  
Baculovirus Expression System ◽  
Baculovirus Expression

scholarly journals InteBac - An integrated bacterial and baculovirus expression vector suite

2020 ◽  
Author(s):  
Veronika Altmannova ◽  
Andreas Blaha ◽  
Susanne Astrinidis ◽  
Heidi Reichle ◽  
John R. Weir
Keyword(s):  
Recombinant Protein ◽  
Insect Cell ◽  
Expression System ◽  
Integrated System ◽  
Expression Vectors ◽  
E Coli ◽  
Baculovirus Expression ◽  
Biological Studies ◽  
Cell Expression ◽  
Insect Cell Expression

The successful production of recombinant protein for biochemical, biophysical and structural biological studies critically depends on the correct expression organism. Currently the most commonly used expression organisms for structural studies are E. coli (ca. 70% of all PDB structures) and the baculovirus/ insect cell expression system (ca. 5% of all PDB structures). While insect cell expression is frequently successful for large eukaryotic proteins, it is relatively expensive and time consuming compared to E. coli expression. Frequently the decision to carry out a baculovirus project means restarting cloning from scratch. Here we describe an integrated system that allows the simultaneous cloning into E. coli and baculovirus expression vectors using the same PCR products. The system offers a flexible array of N- and C-terminal affinity, solublisation and utility tags, and the speed allows expression screening to be completed in E. coli, before carrying out time and cost intensive experiments in baculovirus. Importantly, we describe a means of rapidly generating polycistronic bacterial constructs based on the hugely successful biGBac system, making InteBac of particular interest for researchers working on recombinant protein complexes.

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