Faculty Opinions recommendation of Overexpression of RCN1 and RCN2, rice TERMINAL FLOWER 1/CENTRORADIALIS homologs, confers delay of phase transition and altered panicle morphology in rice.

Abstract Lateral branching is one of the most important traits, which directly determines plant 27 architecture and crop productivity. Commercial watermelon has the characteristics of multiple 28 lateral branches, and it is time-consuming and labor costing to manually remove the lateral 29 branches in traditional watermelon cultivation. In our present study, a lateral branchless trait was 30 identified in watermelon material W CZ, and genetic analysis revealed that it was controlled by a 31 single recessive gene, which named as Clbl . A bulked segregant sequencing (BSA seq) and 32 linkage analysis was conducted to primarily mapping of Clbl on watermelon chromosome 4 33 Next-generation sequencing aided marker discovery and a large mapping population consisting of 34 1406 F 2 plants was used to further mapped the Clbl locus into a 9011 bp candidate region which 35 harbored only one candidate gene Cla018392 encoding a TERMINAL FLOWER 1 gene. Sequence 36 comparison of Cla018392 between two parental lines revealed that there was a SNP detected from 37 C to A in the coding region in the branchless inbred line WCZ , which resulted in a mutation of 38 Alanine (GCA) to Glutamate (GAA) at the fourth exon A dCAPS marker was developed from the 39 SNP locus, which was co-segregated with the branchless phenotype in both BC 1 and F 2 population, 40 and it was also further validated in 152 natural watermelon accessions. qRT PCR and in situ 41 hybridization showed that the expression levels of Cla0 18392 was significantly reduced in the 42 axillary bud and apical bud in the branchless line WCZ Ectopic expression of ClTFL1 in 43 Arabidopsis showed an increased number of lateral branches. The results of this study will be 44 useful for better understanding the molecular mechanism of lateral branch development in 45 watermelon and for the development of marker-assisted selection (MAS) for new branchless 46 watermelon cultivars.

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Sequence and functional analysis of a TERMINAL FLOWER 1 homolog from Brassica juncea: a putative biotechnological tool for flowering time adjustment

GM crops & food ◽

10.1080/21645698.2019.1707340 ◽

2019 ◽

Vol 11 (2) ◽

pp. 79-92

Author(s):

Mohsen Asadi Khanouki ◽

Farkhondeh Rezanejad ◽

Anthony A. Millar

Keyword(s):

Functional Analysis ◽

Flowering Time ◽

Brassica Juncea ◽

Terminal Flower ◽

Terminal Flower 1

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Characterization of the BnA10.tfl1 Gene Controls Determinate Inflorescence Trait in Brassica napus L.

Agronomy ◽

10.3390/agronomy9110722 ◽

2019 ◽

Vol 9 (11) ◽

pp. 722 ◽

Cited By ~ 2

Author(s):

Yongpeng Jia ◽

Kaixiang Li ◽

Haidong Liu ◽

Lingxiong Zan ◽

Dezhi Du

Keyword(s):

Brassica Napus ◽

Shoot Apex ◽

Apical Meristem ◽

Brassica Napus L ◽

Reading Frame ◽

Terminal Flower ◽

Terminal Flower 1 ◽

Oilseed Breeding ◽

Phosphatidylethanolamine Binding Protein

Determinate inflorescences have a significant effect on the genetic improvement of rapeseed, so understanding the molecular function underlying the inflorescence trait may be beneficial to oilseed breeding. A previous study found candidate gene BnTFL1 (Terminal Flower 1) for control of the inflorescence trait on Brassica napus chromosome A10 (16,627–16,847 kb). However, little is known about the function of the BnTFL1 gene in B. napus. In this study, we firstly studied the formation of the shoot apical meristem and gene expression in indeterminate and determinate inflorescences; the results showed that the inflorescence architecture and BnA10.TFL1 expression showed significant differences in the shoot apex at the budding stage. Then, two alleles (named BnA10.TFL1 gene from indeterminate and BnA10.tfl1 gene from determinate) were cloned and sequence-analyzed; the results suggest that the open reading frame of the alleles comprises 537 bp, encodes 178 amino acids containing a conserved phosphatidylethanolamine-binding protein (PEBP) domain, and shares high similarity with Arabidopsis thaliana TFL1. To analyze the function of BnA10.TFL1, the BnA10.TFL1 gene was introduced into the determinate A. thaliana tfl1 mutant and B. napus 571 line by complementation experiment. The determinate traits were restored to indeterminate, and expression of BnA10.TFL1 was increased in the indeterminate shoot apex. These results reveal that BnA10.tfl1 is a gene controlling the determinate inflorescence trait. Moreover, the BnA10.TFL1 protein was localized to the nucleus, cytoplasm, and plasma membrane. Collectively, the results of this study help us to understand the molecular mechanism of determinate inflorescences and will provide a reliable research basis for the application of determinate inflorescences in B. napus.

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