Faculty Opinions recommendation of The integral membrane nucleoporin pom121 functionally links nuclear pore complex assembly and nuclear envelope formation.

All nucleocytoplasmic traffic of macromolecules occurs through nuclear pore complexes (NPCs), which function as stents in the nuclear envelope to keep nuclear pores open but gated. Three studies in this issue (Flemming, D., P. Sarges, P. Stelter, A. Hellwig, B. Böttcher, and E. Hurt. 2009. J. Cell Biol. 185:387–395; Makio, T., L.H. Stanton, C.-C. Lin, D.S. Goldfarb, K. Weis, and R.W. Wozniak. 2009. J. Cell Biol. 185:459–491; Onishchenko, E., L.H. Stanton, A.S. Madrid, T. Kieselbach, and K. Weis. 2009. J. Cell Biol. 185:475–491) further our understanding of the NPC assembly process by reporting what happens when the supply lines of key proteins that provide a foundation for building these marvelous supramolecular structures are disrupted.

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Nuclear envelope-vacuole contacts mitigate nuclear pore complex assembly stress

10.1101/2020.03.23.001719 ◽

2020 ◽

Cited By ~ 1

Author(s):

Christopher L. Lord ◽

Susan R. Wente

Keyword(s):

Saccharomyces Cerevisiae ◽

Nuclear Envelope ◽

Nuclear Pore Complex ◽

Lipid Droplets ◽

Budding Yeast ◽

Nuclear Transport ◽

Nuclear Pore ◽

Cellular Mechanisms ◽

Complex Assembly ◽

Cellular Effects

AbstractThe intricacy of nuclear pore complex (NPC) biogenesis imposes risks of failure that can cause defects in nuclear transport and nuclear envelope morphology, however, cellular mechanisms utilized to alleviate NPC assembly stress are not well-defined. In the budding yeast Saccharomyces cerevisiae, we demonstrate that NVJ1- and MDM1-enriched nuclear envelope (NE)-vacuole contacts increase when NPC assembly is compromised in several nup mutants, including nup116ΔGLFG cells. These interorganelle nucleus-vacuole junctions (NVJs) cooperate with lipid droplets to maintain viability and enhance NPC formation in assembly mutants. Additionally, NVJs function with ATG1 to promote vacuole-dependent remodeling in nup116ΔGLFG cells, which also correlates with proper NPC formation. Importantly, NVJs significantly improve the physiology of NPC assembly mutants, despite having only negligible effects when NPC biogenesis is unperturbed. Collectively, these results define how NE-vacuole interorganelle contacts coordinate responses to mitigate deleterious cellular effects caused by disrupted NPC assembly.SummaryHow cells respond to deleterious effects imposed by disrupted nuclear pore complex (NPC) assembly are not well-defined. The authors demonstrate nuclear envelope-vacuole interactions expand in response to perturbed NPC assembly to promote viability, nuclear envelope remodeling, and proper NPC biogenesis.

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Yeast Nuclear Pore Complex Assembly Defects Determined by Nuclear Envelope Reconstruction

Journal of Structural Biology ◽

10.1006/jsbi.2000.4305 ◽

2000 ◽

Vol 132 (1) ◽

pp. 1-5 ◽

Cited By ~ 17

Author(s):

Natalia Gomez-Ospina ◽

Garry Morgan ◽

Thomas H. Giddings ◽

Buket Kosova ◽

Ed Hurt ◽

...

Keyword(s):

Nuclear Envelope ◽

Nuclear Pore Complex ◽

Nuclear Pore ◽

Complex Assembly ◽

Envelope Reconstruction

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Building a nuclear envelope at the end of mitosis: coordinating membrane reorganization, nuclear pore complex assembly, and chromatin de-condensation

Chromosoma ◽

10.1007/s00412-012-0388-3 ◽

2012 ◽

Vol 121 (6) ◽

pp. 539-554 ◽

Cited By ~ 53

Author(s):

Allana Schooley ◽

Benjamin Vollmer ◽

Wolfram Antonin

Keyword(s):

Nuclear Envelope ◽

Nuclear Pore Complex ◽

Nuclear Pore ◽

Complex Assembly ◽

Membrane Reorganization

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Nuclear envelope–vacuole contacts mitigate nuclear pore complex assembly stress

The Journal of Cell Biology ◽

10.1083/jcb.202001165 ◽

2020 ◽

Vol 219 (12) ◽

Author(s):

Christopher L. Lord ◽

Susan R. Wente

Keyword(s):

Saccharomyces Cerevisiae ◽

Nuclear Envelope ◽

Nuclear Pore Complex ◽

Lipid Droplets ◽

Budding Yeast ◽

Nuclear Pore ◽

Cellular Mechanisms ◽

Yeast Saccharomyces Cerevisiae ◽

Complex Assembly ◽

Cellular Effects

The intricacy of nuclear pore complex (NPC) biogenesis imposes risks of failure that can cause defects in nuclear transport and nuclear envelope (NE) morphology; however, cellular mechanisms used to alleviate NPC assembly stress are not well defined. In the budding yeast Saccharomyces cerevisiae, we demonstrate that NVJ1- and MDM1-enriched NE–vacuole contacts increase when NPC assembly is compromised in several nup mutants, including nup116ΔGLFG cells. These interorganelle nucleus–vacuole junctions (NVJs) cooperate with lipid droplets to maintain viability and enhance NPC formation in assembly mutants. Additionally, NVJs function with ATG1 to remodel the NE and promote vacuole-dependent degradation of specific nucleoporins in nup116ΔGLFG cells. Importantly, NVJs significantly improve the physiology of NPC assembly mutants, despite having only negligible effects when NPC biogenesis is unperturbed. These results therefore define how NE–vacuole interorganelle contacts coordinate responses to mitigate deleterious cellular effects caused by disrupted NPC assembly.

Download Full-text