Faculty Opinions recommendation of Curved DNA molecules migrate anomalously slowly in free solution.

2005 ◽  
Author(s):  
Jim Maher
Keyword(s):  
Free Solution ◽  
Dna Molecules ◽  
Curved Dna

Effect of organic cosolvents on the free solution mobility of curved and normal DNA molecules

2006 ◽  
Vol 27 (8) ◽  
pp. 1462-1470 ◽  
Author(s):  
Yongjun Lu ◽  
Earle Stellwagen ◽  
Nancy C. Stellwagen
Keyword(s):  
Free Solution ◽  
Dna Molecules ◽  
Organic Cosolvents

Dynamics of Curved DNA Molecules:  Prediction and Experiment

2003 ◽  
Vol 125 (37) ◽  
pp. 11160-11161 ◽  
Author(s):  
Tali E. Haran ◽  
Ilana Cohen ◽  
Alexander Spasic ◽  
Kevin Yang ◽  
Udayan Mohanty
Keyword(s):  
Dna Molecules ◽  
Curved Dna

Diffusion coefficient of DNA molecules during free solution electrophoresis

2001 ◽  
Vol 22 (12) ◽  
pp. 2424-2432 ◽  
Author(s):  
Axel E. Nkodo ◽  
Jean M. Garnier ◽  
Bernard Tinland ◽  
Hongji Ren ◽  
Claude Desruisseaux ◽  
...  
Keyword(s):  
Diffusion Coefficient ◽  
Free Solution ◽  
Dna Molecules

TORSIONAL RESONANCE MODE ATOMIC FORCE MICROSCOPY OF A PROTEIN–DNA COMPLEX

NANO ◽  
2008 ◽  
Vol 03 (06) ◽  
pp. 443-448 ◽  
Author(s):  
GILBERTO WEISSMÜLLER ◽  
AYHAN YURTSEVER ◽  
LILIAN T. COSTA ◽  
ANA B. F. PACHECO ◽  
PAULO M. BISCH ◽  
...  
Keyword(s):  
Protein Binding ◽  
Binding Site ◽  
Binding Sites ◽  
Physical Map ◽  
Resonance Mode ◽  
Dna Molecules ◽  
Image Processing Algorithm ◽  
Protein Binding Sites ◽  
Curved Dna ◽  
Atomic Force

Precise mapping of protein-binding sites on DNA is an important application of atomic force microscope (AFM) imaging. For a reliable measurement of distances on curved DNA molecules, an image-processing algorithm is required, which extracts the DNA contour from topographic AFM data. To this end we implemented an image analysis method providing an efficient way to obtain the contour together with a physical map of single and multiple protein-binding sites. This method relies on a calculation of the height profile along the DNA fragment, allowing one to determine the DNA length and the relative position of the binding site occupied by a protein. As a first test, complexes of the LexA repressor protein from the Escherichia coli SOS system and DNA fragments containing a specific LexA binding site (recA operator) were imaged by the torsional resonance mode (TR mode) and analyzed using the specialized algorithm. A topographic height of less than 0.5 nm of the DNA molecules indicates repulsive imaging conditions.

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