Faculty Opinions recommendation of Evidence for epithelial-mesenchymal transitions in adult liver cells.

1. Rabbit anti-(rat foetal liver) serum, absorbed with adult rat liver cells, decreased the electrophoretic mobility of foetal liver cells by 51% and rat hepatoma cells by 45%, indicating the presence of a foetal-type antigen on the hepatoma cell membrane. 2. The chemical nature of the surface antigen was investigated. Incubation with neuraminidase had no effect on adult liver cells but decreased the electrophoretic mobility of foetal liver cells by 51% and of hepatoma cells by 34%; the effect of antiserum was decreased to one-fifth. 3. Sialic acid, or the supernatant from neuraminidase-treated cells, partially blocked the decrease in electrophoretic mobility induced by antiserum. 4. The pH–electrophoretic mobility curves of hepatoma cells treated with antisera were consistent with a sialic acidcontaining antigen on the surface of the tumour cells. 5. Treatment with ribonuclease did not decrease the electrophoretic mobility of adult-liver cells, but decreased that of the foetal liver cells by 17% and hepatoma cells by 29%. 6. In parallel studies made with mouse BP8 ascites-tumour cells ribonuclease decreased the electrophoretic mobility by 39%, that of normal mouse lymph-node cells by 4.8% and allergized mouse lymph-node cells by 13.3%. 7. Trypsin treatment also decreased the electrophoretic mobility of hepatoma cells by 22%.

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Cultivation of embryonic and adult liver cells on a collagen substrate

Experimental Cell Research ◽

10.1016/0014-4827(59)90086-2 ◽

1959 ◽

Vol 17 (3) ◽

pp. 557-560 ◽

Cited By ~ 19

Author(s):

W.D. Hillis ◽

F.B. Bang

Keyword(s):

Liver Cells ◽

Adult Liver ◽

Collagen Substrate

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Long-Term Culture of Human Adult Liver Cells: Morphological Changes Related to in vitro Senescence and Effect of Donor’s Age on Growth Potential

Gerontology ◽

10.1159/000211984 ◽

1973 ◽

Vol 19 (5-6) ◽

pp. 303-313 ◽

Cited By ~ 79

Author(s):

Y. Le Guilly ◽

M. Simon ◽

P. Lenoir ◽

M. Bourel

Keyword(s):

Morphological Changes ◽

Liver Cells ◽

Growth Potential ◽

Adult Liver ◽

Long Term Culture ◽

Human Adult

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Evidence for epithelial-mesenchymal transitions in adult liver cells

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00102.2006 ◽

2006 ◽

Vol 291 (4) ◽

pp. G575-G583 ◽

Cited By ~ 86

Author(s):

Jason K. Sicklick ◽

Steve S. Choi ◽

Marcia Bustamante ◽

Shannon J. McCall ◽

Elizabeth Hernández Pérez ◽

...

Keyword(s):

Epithelial Cells ◽

Expression Profile ◽

Hepatic Stellate Cells ◽

Mesenchymal Cell ◽

Stellate Cells ◽

Liver Cells ◽

Adult Liver ◽

Cell Markers ◽

Epithelial Markers

Both myofibroblastic hepatic stellate cells (HSC) and hepatic epithelial progenitors accumulate in damaged livers. In some injured organs, the ability to distinguish between fibroblastic and epithelial cells is sometimes difficult because cells undergo epithelial-mesenchymal transitions (EMT). During EMT, cells coexpress epithelial and mesenchymal cell markers. To determine whether EMT occurs in adult liver cells, we analyzed the expression profile of primary HSC, two HSC lines, and hepatic epithelial progenitors. As expected, all HSC expressed HSC markers. Surprisingly, these markers were also expressed by epithelial progenitors. In addition, one HSC line expressed typical epithelial progenitor mRNAs, and these epithelial markers were inducible in the second HSC line. In normal and damaged livers, small ductular-type cells stained positive for an HSC marker. In conclusion, HSC and hepatic epithelial progenitors both coexpress epithelial and mesenchymal markers, providing evidence that EMT occurs in adult liver cells.

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