Faculty Opinions recommendation of Serum prolactin concentration and its relationship with dehydroepiandrosterone sulfate concentration in chronic urticaria patients with positive and negative response to autologous serum skin test.

Background: Autologous serum skin test (ASST) is easy to perform, cost effective measure for initial identification of auto-antibody in patients suffering from chronic urticaria which may occur due to autoimmune trigger by spontaneously developed auto-antibodies against FcμRI receptor of skin mast cells.Objective: This review is designed to see the positivity of ASST in patients suffering from chronic urticaria.Materials & Methods: This a retrospective data analysis conducted from the records of patients suffered from at least two episodes of urticaria or more in a week for 6 weeks and who did ASST aged more than 10 years between July 2015 to June 2017. These subjects were not pregnant or lactating mother, did not had urticarial vasculitis, predominant co-existing physical urticaria and had negative Hepatitis B antigen and anti-nuclear antibody reports.Results: Total 53 were included in this study of them 33 were female. Mean age was 32 years with a standard deviation (SD) of 11 years. Among study patients 16 (30.2% of total) had a positive ASST result. No age or sex difference was observed in positive ASST cases. In positive cases significant (p-value:<0.001) mean induration difference of 2.75mm with a SD of 1.00 mm observed than induration produced by negative control.Conclusion: ASST is easy and effective tool for initial identification of auto-reactive urticaria.J MEDICINE JUL 2018; 19 (2) : 100-103

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Autologous Whole Blood Injections to Patients with Chronic Urticaria and a Positive Autologous Serum Skin Test: A Placebo-Controlled Trial

Dermatology ◽

10.1159/000090656 ◽

2006 ◽

Vol 212 (2) ◽

pp. 150-159 ◽

Cited By ~ 70

Author(s):

P. Staubach ◽

K. Onnen ◽

A. Vonend ◽

M. Metz ◽

F. Siebenhaar ◽

...

Keyword(s):

Skin Test ◽

Chronic Urticaria ◽

Whole Blood ◽

Controlled Trial ◽

Autologous Serum ◽

Autologous Serum Skin Test ◽

Autologous Whole Blood

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HLA among Brazilian patients with spontaneous chronic urticaria and positive autologous serum skin test

Anais Brasileiros de Dermatologia ◽

10.1590/s0365-05962012000400010 ◽

2012 ◽

Vol 87 (4) ◽

pp. 578-583 ◽

Cited By ~ 2

Author(s):

Zamir Calamita ◽

Andrea Bronhara Pelá ◽

Márcia Gamberini ◽

Wilson Baleotti Júnior ◽

Odilon Marques de Almeida Filho ◽

...

Keyword(s):

Major Histocompatibility Complex ◽

Skin Test ◽

Chronic Urticaria ◽

Population Group ◽

Autologous Serum ◽

Major Histocompatibility ◽

Allele Distribution ◽

Autologous Serum Skin Test ◽

Histocompatibility Complex ◽

Oligonucleotide Hybridization

BACKGROUND: An association between class I and II alleles of the major histocompatibility complex and idiopathic chronic urticaria has previously been observed in different populations, but there are still no studies on Brazilian populations in this regard. OBJECTIVE: The involvement of the major histocompatibility complex classes I and II (loci A, B and DR) in Brazilian patients with idiopathic chronic urticaria and a positive autologous serum skin test was investigated and compared with a healthy population group. METHODS: DNA was extracted from the blood of 42 patients with idiopathic chronic urticaria and major histocompatibility complex classes I and II alleles were determined using the polymerase chain reaction and a laboratory test for oligonucleotide hybridization using a single-filament probe. The frequencies of these alleles in patients with chronic urticaria were compared with the frequencies in 1000 genetically unrelated voluntary blood donors from the same region of Brazil. The diagnosis of idiopathic chronic urticaria was based on the patients' clinical history and routine laboratory tests. Only the patients with positive autologous serum skin test were selected. The allele distribution resulted from the patient and control groups were analyzed using odds ratios and 95% confidence intervals. RESULTS: No statistically significant differences were found between the positive autologous serum skin test patients with chronic urticaria and the control group. CONCLUSIONS: We found that in this population group, there was no specific association between the HLA alleles studied and chronic urticaria. We believe that further population studies are needed in order to investigate the possible existence of this association.

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A study of autologous serum skin test and autologous serum therapy in chronic urticaria

International Journal of Research in Dermatology ◽

10.18203/issn.2455-4529.intjresdermatol20181828 ◽

2018 ◽

Vol 4 (2) ◽

pp. 246

Author(s):

Bharti Patel ◽

Komal Kapadia ◽

Khushbu Chauhan ◽

Neela Bhuptani

Keyword(s):

Skin Test ◽

Chronic Urticaria ◽

Quality Index ◽

Life Quality ◽

Autologous Serum ◽

Effective Parameters ◽

Positive Skin ◽

Autologous Serum Skin Test ◽

Before And After ◽

Life Quality Index

Background: Chronic urticaria (CU) is a vexing problem and patients suffer from the morbidity that arise from irritable itch and wheals and are also subjected to a huge antihistamine pill burden. A subset of patients with CU may have an autoimmune basis for their condition, as shown by a positive skin test to ASST. The objective of the study was to compare efficacy of AST in ASST positive and negative patients and its impact on dermatology life quality index (DLQI) in patients, before and after AST.Methods: A prospective, interventional study was conducted in the Department of dermatology (Skin) OPD of our institute from October 2016 to October 2017. Fifty patients were selected randomly and antihistaminics were withdrawn before ASST. Test was performed in all patients and AST was given for 9 weeks in both (ASST positive & negative) groups, along with tablet levocetrizine on demand basis and followed for 4 weeks after completion of 9 weeks of therapy. Total severity score (TSS), urticaria activity score (UAS), dermatologic life quality index (DLQI) were used as primary effective parameters and were recorded at baseline and weekly after each injections of AST.Results: UAS and TSS showed significant improvement (>50%) after 5th week in both group patients. DLQI showed higher improvement in ASST positive patients.Conclusions: We found significant improvement in ASST positive and ASST negative patients but ASST positive patients required more time to experience the benefit of AST.

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Association between Clinico-epidemiological Features in Chronic Urticaria with Autologous Serum Skin Test: A Cross-sectional Observational Study

JOURNAL FOR CLINICAL AND DIAGNOSTIC RESEARCH ◽

10.7860/jcdr/2021/48816.15109 ◽

2021 ◽

Author(s):

Usha Rani Tirupathi ◽

Bhagya Rekha Manchiryala ◽

Sruthi Kareddy

Keyword(s):

Skin Test ◽

Chronic Urticaria ◽

Autologous Serum ◽

Clinical Test ◽

Cross Sectional ◽

Autologous Serum Skin Test ◽

Epidemiological Features ◽

Chronic Inducible Urticaria ◽

Autoimmune Urticaria ◽

Significant Difference

Introduction: Urticaria is a frequent and heterogeneous skin disease characterised by the development of wheals (hives), angioedema, or both. Chronic urticaria is characterised by the occurrence of wheals lasting less than 24 hours, with or without angioedema occurring daily or almost daily lasting more than six weeks. The two subtypes of chronic urticaria are Chronic Spontaneous Urticaria (CSU) and Chronic Inducible Urticaria (CIU). Autologous Serum Skin Test (ASST) is the simplest and the best in-vivo clinical test for the detection of basophil histamine- releasing activity. ASST has a sensitivity of approximately 70% and a specificity of 80%. Aim: To study the association between clinico-epidemiological features of CSU with positive and negative ASST. Materials and Methods: The cross-sectional study was conducted on 80 clinically diagnosed cases of CSU attending the Department of Dermatology Venereology Leprology Outpatient Department at Mahatma Gandhi Memorial Hospital (Kakatiya Medical College), Warangal, Telangana, India; during the period of January 2016 to September 2017. The detailed history was taken; complete physical and cutaneous examination and laboratory investigations like Complete Blood Picture (CBP), Absolute Eosinophil Count (AEC), Erythrocytic Sedimentation Rate (ESR), Thyroid Function Test (TFT) were carried out. ASST was done and read after 30 minutes. Chi- square test was applied and the results analysed using Statistical Package for the Social Sciences (SPSS) version 10.0. Results: Out of total 80 patients included in the study, ASST was positive in 36 (45%) and negative in 44 (55%). ASST positive patients showed longer duration of the disease with increased frequency of attacks and longer duration of wheals. However, both ASST positive and negative groups did not show statistically significant difference in the age of occurrence, gender, angioedema, dermographism. Conclusion: Autoimmune urticaria has no distinctive clinical features. ASST positive, autoimmune urticaria patients have more severe disease with greater impact on quality of life. Thus, they may need more aggressive treatment.

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