scholarly journals Autologous Serum Skin Test for Initial Identification of Auto- Antibodies in Chronic Urticaria

2018 ◽  
Vol 19 (2) ◽  
pp. 100-103
Author(s):  
Mohammad Nura Alam ◽  
Mohammad Ali ◽  
Mohammad Moniruzzaman Khan ◽  
Keya Tarafder ◽  
Goutam Kumar Acherjya ◽  
...  
Keyword(s):  
Skin Test ◽  
Chronic Urticaria ◽  
Cost Effective ◽  
Autologous Serum ◽  
P Value ◽  
Effective Measure ◽  
Autologous Serum Skin Test ◽  
Physical Urticaria ◽  
Retrospective Data Analysis ◽  
Initial Identification

Background: Autologous serum skin test (ASST) is easy to perform, cost effective measure for initial identification of auto-antibody in patients suffering from chronic urticaria which may occur due to autoimmune trigger by spontaneously developed auto-antibodies against FcμRI receptor of skin mast cells.Objective: This review is designed to see the positivity of ASST in patients suffering from chronic urticaria.Materials & Methods: This a retrospective data analysis conducted from the records of patients suffered from at least two episodes of urticaria or more in a week for 6 weeks and who did ASST aged more than 10 years between July 2015 to June 2017. These subjects were not pregnant or lactating mother, did not had urticarial vasculitis, predominant co-existing physical urticaria and had negative Hepatitis B antigen and anti-nuclear antibody reports.Results: Total 53 were included in this study of them 33 were female. Mean age was 32 years with a standard deviation (SD) of 11 years. Among study patients 16 (30.2% of total) had a positive ASST result. No age or sex difference was observed in positive ASST cases. In positive cases significant (p-value:<0.001) mean induration difference of 2.75mm with a SD of 1.00 mm observed than induration produced by negative control.Conclusion: ASST is easy and effective tool for initial identification of auto-reactive urticaria.J MEDICINE JUL 2018; 19 (2) : 100-103

scholarly journals Autologous Serum Skin Test in Chronic Idiopathic Urticaria

2016 ◽  
Vol 12 (2) ◽  
pp. 6-10 ◽  
Author(s):  
Kumar Pokhrel ◽  
S. Subedi ◽  
S. Acharya
Keyword(s):  
Skin Test ◽  
Chronic Urticaria ◽  
Cost Effective ◽  
Activity Score ◽  
Autologous Serum ◽  
Cross Sectional ◽  
Dermatology Department ◽  
Autologous Serum Skin Test ◽  
Autoimmune Urticaria ◽  
Urticaria Activity Score

Background: Urticaria is a short-lived swelling of skin and mucosa due to plasma leakage by immune and non immune mediated activation and released of mediators from mast cell and basophil. Autoimmune urticaria tends to have a high itch and wheal score than other type of urticaria. Its diagnosis is practically relied upon clinical suspicion and autologous serum skin test. Autologous serum skin test (ASST) is the simple and cost effective test to differentiate autoimmune urticaria from the bulk of chronic urticaria patients. Objective: To compare the features of chronic urticaria in patients having positive versus negative autologous serum skin test (ASST).Materials and methods: Cross-sectional hospital based study was conducted among 90 chronic urticaria patients (CIU) attending the outpatient dermatology department of Nepalgunj Medical College, Nepalgunj, during one year period. The study was conducted after ethical approval from the institutional committee. The patients were diagnosed on the basis of the appearance of continuous or recurrent hives with or without angioedema for more than 6 weeks. Patients who suffered from either acute urticaria or urticarial vasculitis or physical urticaria or other systemic diseases known to cause urticaria were excluded. Standard tools and techniques were used to prepare antilogous serum and injection of the serum and interpretation of the result. The test result was Interpreted as positive and negative autologous serum skin test.Results: ASST was positive in 42% of the patients and negative in 58% of the patients. The ASST-positive patients had a higher mean urticaria activity score and median duration of wheals in comparison with the ASST-negative patients. Wheals lasted for significantly longer duration in patients with positive ASST. Patients with positive ASST had more frequent attacks which was statistically significant compared to the ASST-negative group. The mean urticaria activity score was significantly higher in the ASST-positive patients than that in the ASST-negative patients.Conclusions: Autologous serum skin test may be a useful screening test for autoimmune urticaria and may be used as a simple and cost-effective test for the classification of chronic urticaria.JNGMC Vol. 12 No. 2 December 2014, page: 6-10

scholarly journals Chronic urticaria: a clinico-etiological study and autologous serum skin test role in chronic idiopathic urticaria

2021 ◽  
Vol 7 (4) ◽  
pp. 552
Author(s):  
Purnachandra Badabagni ◽  
Sruthi Thammineedi ◽  
Ramadevi Birudala
Keyword(s):  
Skin Test ◽  
Chronic Urticaria ◽  
Chronic Idiopathic Urticaria ◽  
Autologous Serum ◽  
Thyroid Function Tests ◽  
Cross Sectional ◽  
Autologous Serum Skin Test ◽  
Physical Urticaria ◽  
Autoimmune Urticaria ◽  
Number Of Patients

<p class="abstract"><strong>Background:</strong> Appearance of wheals daily for more than six weeks is chronic urticarial (CU). No cause is identified in about 50-70% of chronic urticaria patients and are labelled as chronic idiopathic urticaria (CIU). The aim of the present study is to study the clinical and etiological pattern of chronic urticaria and to find out the incidence of autoimmune urticaria by performing autologous serum skin test (ASST) in patients with CIU.</p><p class="abstract"><strong>Methods:</strong> This was a cross sectional study enrolling 100 chronic urticaria patients satisfying including and excluding criteria. The study was done for a period of 1 year. ASST was done in all the CIU patients after recording detailed history, complete physical and systemic examination.</p><p class="abstract"><strong>Results:</strong> Most of the patients (33%) were in 21-30 years age group with female preponderance (66%). Students (38%) followed by house wives (27%) were majorly involved. 21% patients had history of atopy and 8% had abnormal thyroid function tests. Causative factors noticed in 46% patients and remaining were idiopathic (54%). Infections (32.6%) constituted the major etiological factor followed by physical urticaria (30.4%), food (23.9%), medication (11%) and inhalants (2.1%). In infective agents, 46.6% were bacterial followed by helminthic (33.3%). In 29.6% of CIU patients, the ASST was positive indicating auto-immune urticaria.</p><p class="abstract"><strong>Conclusions:</strong> The etiology cannot be identified in most number of patients and hence they were labelled as CIU and the common causative agents observed were infections followed by physical urticaria, food and medication. ASST is considered as the relevant screening test to detect autoimmune urticaria.</p>

scholarly journals Clinical Outcome of Autologous Serum Therapy in Chronic Idiopathic Urticaria

2017 ◽  
Vol 15 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Dharmendra Karn ◽  
Shekhar KC
Keyword(s):  
Skin Prick Test ◽  
Chronic Urticaria ◽  
Statistical Significance ◽  
Cost Effective ◽  
Chronic Idiopathic Urticaria ◽  
Autologous Serum ◽  
P Value ◽  
Prick Test ◽  
H1 Antihistamines ◽  
Serum Therapy

Background: Quality of life in chronic idiopathic urticaria is hampered as efficacy of H1-antihistamines is limited. Autologous serum containing tolerance-generating anti-idiotype antibodies is a novel and cost-effective therapy. This study was conducted to evaluate the efficacy of autologous serum therapy (AST) among chronic urticaria patients with autologous skin prick test positive and negative status.Methods: Untreated 102 patients of chronic urticaria were enrolled in a non-randomized interventional study. Patients were categorized into two groups based on autologous serum skin prick test as test positive (ASST +) and test negative (ASST -). Patients were then treated with intramuscular injection of 0.05ml per kg body weight of autologous serum weekly for 10 weeks. Urticaria activity scoring (UAS) tool was used for quantification of the symptoms. Weekly recording of UAS (range: 0-42) was made before the therapy (baseline) and during the therapy for 10 weeks.Results: Significant improvement with AST in the mean UAS was noted from baseline to 10 weeks in both the group of patients (14.6 ± 6.3 and 10.2 ± 5.1 for ASST+ group; 16.9 ± 7.8 and 8.6 ± 4.8 for ASST- group; at baseline and 10 weeks, respectively (p-value for both <0.05)). However no statistical significance was found while comparing the efficacy of the therapy against ASST + and ASST - Groups (p-value > 0.05).Conclusions: Irrespective of autologous skin prick test results, autologous serum therapy showed significant improvement in patients with chornic idiopathic urticaria. AST can, thus, be an effective treatment modality for it.

The autologous serum skin test in the follow-up of patients with chronic urticaria

Allergy ◽  
2005 ◽  
Vol 60 (2) ◽  
pp. 256-258 ◽  
Author(s):  
A. Fusari ◽  
C. Colangelo ◽  
F. Bonifazi ◽  
L. Antonicelli
Keyword(s):  
Skin Test ◽  
Chronic Urticaria ◽  
Autologous Serum ◽  
Autologous Serum Skin Test

Autologous Whole Blood Injections to Patients with Chronic Urticaria and a Positive Autologous Serum Skin Test: A Placebo-Controlled Trial

Dermatology ◽  
2006 ◽  
Vol 212 (2) ◽  
pp. 150-159 ◽  
Author(s):  
P. Staubach ◽  
K. Onnen ◽  
A. Vonend ◽  
M. Metz ◽  
F. Siebenhaar ◽  
...  
Keyword(s):  
Skin Test ◽  
Chronic Urticaria ◽  
Whole Blood ◽  
Controlled Trial ◽  
Autologous Serum ◽  
Autologous Serum Skin Test ◽  
Autologous Whole Blood

scholarly journals HLA among Brazilian patients with spontaneous chronic urticaria and positive autologous serum skin test

2012 ◽  
Vol 87 (4) ◽  
pp. 578-583 ◽  
Author(s):  
Zamir Calamita ◽  
Andrea Bronhara Pelá ◽  
Márcia Gamberini ◽  
Wilson Baleotti Júnior ◽  
Odilon Marques de Almeida Filho ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
Skin Test ◽  
Chronic Urticaria ◽  
Population Group ◽  
Autologous Serum ◽  
Major Histocompatibility ◽  
Allele Distribution ◽  
Autologous Serum Skin Test ◽  
Histocompatibility Complex ◽  
Oligonucleotide Hybridization

BACKGROUND: An association between class I and II alleles of the major histocompatibility complex and idiopathic chronic urticaria has previously been observed in different populations, but there are still no studies on Brazilian populations in this regard. OBJECTIVE: The involvement of the major histocompatibility complex classes I and II (loci A, B and DR) in Brazilian patients with idiopathic chronic urticaria and a positive autologous serum skin test was investigated and compared with a healthy population group. METHODS: DNA was extracted from the blood of 42 patients with idiopathic chronic urticaria and major histocompatibility complex classes I and II alleles were determined using the polymerase chain reaction and a laboratory test for oligonucleotide hybridization using a single-filament probe. The frequencies of these alleles in patients with chronic urticaria were compared with the frequencies in 1000 genetically unrelated voluntary blood donors from the same region of Brazil. The diagnosis of idiopathic chronic urticaria was based on the patients' clinical history and routine laboratory tests. Only the patients with positive autologous serum skin test were selected. The allele distribution resulted from the patient and control groups were analyzed using odds ratios and 95% confidence intervals. RESULTS: No statistically significant differences were found between the positive autologous serum skin test patients with chronic urticaria and the control group. CONCLUSIONS: We found that in this population group, there was no specific association between the HLA alleles studied and chronic urticaria. We believe that further population studies are needed in order to investigate the possible existence of this association.

Diagnostic features of chronic urticaria

2016 ◽  
Vol 19 (5) ◽  
pp. 290-295
Author(s):  
N. G Kochergin ◽  
P. V Kolkhir ◽  
Olga A. Kosoukhova
Keyword(s):  
Chronic Urticaria ◽  
Eosinophil Cationic Protein ◽  
Autologous Serum ◽  
Diagnostic Features ◽  
Cationic Protein ◽  
Complement Components ◽  
Autologous Serum Skin Test ◽  
Acute Urticaria ◽  
Physical Urticaria ◽  
Working Age

Urticaria is etiologically heterogeneous disease, where the main clinical sign is a wheal. For the duration of the disease course secrete acute and chronic urticaria. If urticaria elements in the skin of the patient are observedfrom several hours to 6 weeks, it is classified as acute urticaria, cutaneous if the process continues for more than 6 weeks, it is a chronic urticaria. The aim of our work was the optimization of diagnosis of chronic urticaria on the basis of the study of clinical and allergological and immunological characteristics of the testing results. The study involved 60 patients between the ages of 18 to 73 years with confirmed clinical diagnosis of chronic urticaria. The study was conducted in the clinic of skin and venereal diseases ofI.M. Sechenov First Medical State Medical University. Conducted clinical and anamnestic examination: medical history, assessment of complaints, determining the severity of urticaria (UAS7), quality of life (CU-Q2oL, DLQI), control of the symptoms of urticaria (UCT); laboratory tests: clinical blood test, C-reactive protein, thyroid hormones and antibodies to thyroid structures, tests to exclude physical urticaria, autologous serum skin test, D-dimer, rheumatoid factor, eosinophil-cationic protein, the total of IgE, antinuclear antibodies , C3 / C4 complement components, protein fractions, coagulation, urinalysis, general analysis offeces, feces on eggs of worms and protozoa. The study was conducted within 1 month and included diagnostic consultation period and 5 consultations every 7 days. Identified specific clinical and laboratory features that can be assigned to the diagnostic criteria for autoimmune form of chronic urticaria, which will continue to pick up these patients rational treatment. The high prevalence of urticaria, a variety offorms of the disease, the presence ofpathology predominantly in patients of working age, often ineffective diagnostic actions cause the urgency of the problem and the need for further study of this disease.

scholarly journals A study of autologous serum skin test and autologous serum therapy in chronic urticaria

2018 ◽  
Vol 4 (2) ◽  
pp. 246
Author(s):  
Bharti Patel ◽  
Komal Kapadia ◽  
Khushbu Chauhan ◽  
Neela Bhuptani
Keyword(s):  
Skin Test ◽  
Chronic Urticaria ◽  
Quality Index ◽  
Life Quality ◽  
Autologous Serum ◽  
Effective Parameters ◽  
Positive Skin ◽  
Autologous Serum Skin Test ◽  
Before And After ◽  
Life Quality Index

<p class="abstract"><strong>Background:</strong> Chronic urticaria (CU) is a vexing problem and patients suffer from the morbidity that arise from irritable itch and wheals and are also subjected to a huge antihistamine pill burden. A subset of patients with CU may have an autoimmune basis for their condition, as shown by a positive skin test to ASST. The objective of the study was to compare efficacy of AST in ASST positive and negative patients and its impact on dermatology life quality index (DLQI) in patients, before and after AST.</p><p class="abstract"><strong>Methods:</strong> A prospective, interventional study was conducted in the Department of dermatology (Skin) OPD of our institute from October 2016 to October 2017. Fifty patients were selected randomly and antihistaminics were withdrawn before ASST. Test was performed in all patients and AST was given for 9 weeks in both (ASST positive &amp; negative) groups, along with tablet levocetrizine on demand basis and followed for 4 weeks after completion of 9 weeks of therapy. Total severity score (TSS), urticaria activity score (UAS), dermatologic life quality index (DLQI) were used as primary effective parameters and were recorded at baseline and weekly after each injections of AST.<strong></strong></p><p class="abstract"><strong>Results:</strong> UAS and TSS showed significant improvement (&gt;50%) after 5th week in both group patients. DLQI showed higher improvement in ASST positive patients.</p><p class="abstract"><strong>Conclusions:</strong> We found significant improvement in ASST positive and ASST negative patients but ASST positive patients required more time to experience the benefit of AST.</p><p class="abstract"> </p>

scholarly journals Association between Clinico-epidemiological Features in Chronic Urticaria with Autologous Serum Skin Test: A Cross-sectional Observational Study

2021 ◽  
Author(s):  
Usha Rani Tirupathi ◽  
Bhagya Rekha Manchiryala ◽  
Sruthi Kareddy
Keyword(s):  
Skin Test ◽  
Chronic Urticaria ◽  
Autologous Serum ◽  
Clinical Test ◽  
Cross Sectional ◽  
Autologous Serum Skin Test ◽  
Epidemiological Features ◽  
Chronic Inducible Urticaria ◽  
Autoimmune Urticaria ◽  
Significant Difference

Introduction: Urticaria is a frequent and heterogeneous skin disease characterised by the development of wheals (hives), angioedema, or both. Chronic urticaria is characterised by the occurrence of wheals lasting less than 24 hours, with or without angioedema occurring daily or almost daily lasting more than six weeks. The two subtypes of chronic urticaria are Chronic Spontaneous Urticaria (CSU) and Chronic Inducible Urticaria (CIU). Autologous Serum Skin Test (ASST) is the simplest and the best in-vivo clinical test for the detection of basophil histamine- releasing activity. ASST has a sensitivity of approximately 70% and a specificity of 80%. Aim: To study the association between clinico-epidemiological features of CSU with positive and negative ASST. Materials and Methods: The cross-sectional study was conducted on 80 clinically diagnosed cases of CSU attending the Department of Dermatology Venereology Leprology Outpatient Department at Mahatma Gandhi Memorial Hospital (Kakatiya Medical College), Warangal, Telangana, India; during the period of January 2016 to September 2017. The detailed history was taken; complete physical and cutaneous examination and laboratory investigations like Complete Blood Picture (CBP), Absolute Eosinophil Count (AEC), Erythrocytic Sedimentation Rate (ESR), Thyroid Function Test (TFT) were carried out. ASST was done and read after 30 minutes. Chi- square test was applied and the results analysed using Statistical Package for the Social Sciences (SPSS) version 10.0. Results: Out of total 80 patients included in the study, ASST was positive in 36 (45%) and negative in 44 (55%). ASST positive patients showed longer duration of the disease with increased frequency of attacks and longer duration of wheals. However, both ASST positive and negative groups did not show statistically significant difference in the age of occurrence, gender, angioedema, dermographism. Conclusion: Autoimmune urticaria has no distinctive clinical features. ASST positive, autoimmune urticaria patients have more severe disease with greater impact on quality of life. Thus, they may need more aggressive treatment.

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