Faculty Opinions recommendation of Improved assessment of plasmodium vivax response to antimalarial drugs by a colorimetric double-site plasmodium lactate dehydrogenase antigen capture enzyme-linked immunosorbent assay.

2007 ◽  
Author(s):  
Ric Price
Keyword(s):  
Lactate Dehydrogenase ◽  
Plasmodium Vivax ◽  
Immunosorbent Assay ◽  
Antimalarial Drugs ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigen Capture

scholarly journals Improved Assessment of Plasmodium vivax Response to Antimalarial Drugs by a Colorimetric Double-Site Plasmodium Lactate Dehydrogenase Antigen Capture Enzyme-Linked Immunosorbent Assay

2007 ◽  
Vol 51 (6) ◽  
pp. 2112-2116 ◽  
Author(s):  
Pierre Druilhe ◽  
Philippe Brasseur ◽  
Catherine Blanc ◽  
Michael Makler
Keyword(s):  
Lactate Dehydrogenase ◽  
Plasmodium Vivax ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Response Curves ◽  
In Vitro Susceptibility ◽  
Poor Growth ◽  
Antigen Capture

ABSTRACT The occurrence of Plasmodium vivax resistance to chloroquine has been reported in several countries of Asia and South America. However, the resistance of P. vivax is insufficiently documented for three reasons: it has received far less attention than P. falciparum; in vivo investigations are handicapped by the existence of hypnozoites, which make it difficult to distinguish between recrudescences due to drug failure and relapses due to dormant forms in the liver; and in vitro studies are greatly limited by the poor growth of P. vivax. We report on the adaptation to P. vivax of a colorimetric double-site Plasmodium lactate dehydrogenase antigen capture enzyme-linked immunosorbent assay previously developed for P. falciparum. The assay proved remarkably sensitive, as under optimal conditions it could detect P. vivax parasitemia levels as low as 10−8. The technique, which relies on the detection of protein synthesis by the parasite, yielded steep drug-response curves, leading to the precise determination of the 50% inhibitory concentrations for a high proportion of isolates. Chloroquine-resistant parasites were identified in an area where this phenomenon had been documented by in vivo methods. Thus, the results indicate that the in vitro susceptibility of P. vivax can now be monitored easily and efficiently. The data suggest that the threshold of resistance is similar to that of P. falciparum, i.e., in the range of 100 nM for chloroquine and 15 nM for pyronaridine. However, further studies are required to precisely define the cutoff for resistance and the sensitivity to each drug.

scholarly journals Validation of an Enzyme-Linked Immunosorbent Assay That Detects Histoplasma capsulatum Antigenuria in Colombian Patients with AIDS for Diagnosis and Follow-Up during Therapy

2014 ◽  
Vol 21 (9) ◽  
pp. 1364-1368 ◽  
Author(s):  
Diego H. Caceres ◽  
Christina M. Scheel ◽  
Ángela M. Tobón ◽  
Angela Ahlquist Cleveland ◽  
Ángela Restrepo ◽  
...  
Keyword(s):  
Clinical Improvement ◽  
Immunosorbent Assay ◽  
Histoplasma Capsulatum ◽  
Response To Therapy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigen Test ◽  
Content Type ◽  
Antigen Capture

ABSTRACTWe validated an antigen capture enzyme-linked immunosorbent assay (ELISA) in Colombian persons with AIDS and proven histoplasmosis and evaluated the correlation between antigenuria and clinical improvement during follow-up. The sensitivity of theHistoplasma capsulatumELISA was 86%, and the overall specificity was 94%. The antigen test successfully monitored the response to therapy.

Identification of Plasmodium Vivax Sporozoites in Mosquitoes using an Enzyme-Linked Immunosorbent Assay

1985 ◽  
Vol 34 (6) ◽  
pp. 1048-1054 ◽  
Author(s):  
R. A. Wirtz ◽  
T. R. Burkot ◽  
W. E. Collins ◽  
R. G. Andre ◽  
D. R. Roberts ◽  
...  
Keyword(s):  
Plasmodium Vivax ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Development of an antigen-capture enzyme-linked immunosorbent assay for Clostridium perfringens beta2-toxin in porcine feces and the neonatal piglet intestine

2012 ◽  
Vol 24 (5) ◽  
pp. 895-902 ◽  
Author(s):  
Jasmina Kircanski ◽  
Douglas Hodgins ◽  
Glenn Soltes ◽  
Yanlong Pei ◽  
Valeria R. Parreira ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Protease Activity ◽  
Limit Of Detection ◽  
Intestinal Content ◽  
Enzyme Linked Immunosorbent Assay ◽  
Neonatal Piglet ◽  
Antigen Capture ◽  
Field Samples ◽  
Intestinal Contents ◽  
Beta2 Toxin

An enzyme-linked immunosorbent assay (ELISA) was developed for detection and quantitation of beta2-toxin in neonatal piglet intestinal contents. Polystyrene plates were coated with polyclonal capture antibodies prepared against consensus recombinant beta2-toxin. The ELISA was developed using consensus recombinant beta2-toxin, atypical recombinant beta2-toxin, purified consensus native beta2-toxin, and field samples of neonatal porcine intestinal contents. Captured antigen was detected using a horseradish peroxidase–labeled monoclonal antibody against consensus recombinant beta2-toxin. The limit of detection of the ELISA for consensus beta2-toxin was between 2.0 and 3.5 ng/ml. The ELISA detected atypical recombinant beta2-toxin only weakly. Optical density was protein concentration dependent. The test confirmed differences between consensus and atypical recombinant beta2-toxin, but similar results obtained when testing pure consensus recombinant beta2-toxin and native beta2-toxin. Results obtained from intestinal content samples, particularly from the small intestine, were highly inconsistent and suggested variable protease activity. Addition of protease inhibitors partially prevented degradation of the toxin; however, sample processing at low temperature, at a lower pH (citrate buffer with 5% of bovine serum albumin, pH 6.1), and “cold incubation” of applied antigens abolished protease activity. The recombinant toxin was preserved in spiked intestinal samples by freezing at −70°C, suggesting that necropsy samples can be stored frozen for periodic testing. With appropriate sample preparation, antigen-capture ELISA can detect beta2-toxin in the intestinal content and feces of neonatal piglets.

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