Characterization of Nitrate-Dependent As(III)-Oxidizing Communities in Arsenic-Contaminated Soil and Investigation of Their Metabolic Potentials by the Combination of DNA-Stable Isotope Probing and Metagenomics

2020 ◽  
Vol 54 (12) ◽  
pp. 7366-7377 ◽  
Author(s):  
Miaomiao Zhang ◽  
Zhe Li ◽  
Max M. Häggblom ◽  
Lily Young ◽  
Zijun He ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Contaminated Soil ◽  
Stable Isotope Probing

Effect of Incubation Conditions on the Enrichment of Pyrene-degrading Bacteria Identified by Stable-isotope Probing in an Aged, PAH-contaminated Soil

2007 ◽  
Vol 56 (2) ◽  
pp. 341-349 ◽  
Author(s):  
Maiysha D. Jones ◽  
David R. Singleton ◽  
Darryl P. Carstensen ◽  
Sabrina N. Powell ◽  
Julie S. Swanson ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Contaminated Soil ◽  
Stable Isotope Probing ◽  
Degrading Bacteria

scholarly journals Stable-isotope probing of the polycyclic aromatic hydrocarbon-degrading bacterial guild in a contaminated soil

2011 ◽  
Vol 13 (10) ◽  
pp. 2623-2632 ◽  
Author(s):  
Maiysha D. Jones ◽  
Douglas W. Crandell ◽  
David R. Singleton ◽  
Michael D. Aitken
Keyword(s):  
Stable Isotope ◽  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Contaminated Soil ◽  
Stable Isotope Probing ◽  
Polycyclic Aromatic

scholarly journals Functional characterization of an anaerobic benzene-degrading enrichment culture by DNA stable isotope probing

2010 ◽  
Vol 12 (2) ◽  
pp. 401-411 ◽  
Author(s):  
Steffi Herrmann ◽  
Sabine Kleinsteuber ◽  
Antonis Chatzinotas ◽  
Steffen Kuppardt ◽  
Tillmann Lueders ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Enrichment Culture ◽  
Functional Characterization ◽  
Stable Isotope Probing

Characterization of Tetracycline-resistant Microbiome in Soil-plant Systems by Combination of H218O-Based DNA-Stable Isotope Probing and Metagenomics

2021 ◽  
pp. 126440
Author(s):  
Haonan Fan ◽  
Shanghua Wu ◽  
Wenxu Dong ◽  
Xianglong Li ◽  
Yuzhu Dong ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Probing

scholarly journals Characterization of growing bacterial populations in McMurdo Dry Valley soils through stable isotope probing with18O-water

2014 ◽  
Vol 89 (2) ◽  
pp. 415-425 ◽  
Author(s):  
Egbert Schwartz ◽  
David J. Van Horn ◽  
Heather N. Buelow ◽  
Jordan G. Okie ◽  
Michael N. Gooseff ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Probing ◽  
Bacterial Populations ◽  
Dry Valley

scholarly journals Diversity of Five Anaerobic Toluene-Degrading Microbial Communities Investigated Using Stable Isotope Probing

2011 ◽  
Vol 78 (4) ◽  
pp. 972-980 ◽  
Author(s):  
Weimin Sun ◽  
Alison M. Cupples
Keyword(s):  
Stable Isotope ◽  
Microbial Communities ◽  
Contaminated Soil ◽  
Agricultural Soil ◽  
Stable Isotope Probing ◽  
Functional Gene ◽  
Toluene Degradation ◽  
Content Type ◽  
The Family ◽  
Benzylsuccinate Synthase

ABSTRACTTime-series DNA-stable isotope probing (SIP) was used to identify the microbes assimilating carbon from [13C]toluene under nitrate- or sulfate-amended conditions in a range of inoculum sources, including uncontaminated and contaminated soil and wastewater treatment samples. In all, five different phylotypes were found to be responsible for toluene degradation, and these included previously identified toluene degraders as well as novel toluene-degrading microorganisms. In microcosms constructed from granular sludge and amended with nitrate, the putative toluene degraders were classified in the genusThauera, whereas in nitrate-amended microcosms constructed from a different source (agricultural soil), microorganisms in the familyComamonadaceae(genus unclassified) were the key putative degraders. In one set of sulfate-amended microcosms (agricultural soil), the putative toluene degraders were identified as belonging to the classClostridia(genusDesulfosporosinus), while in other sulfate-amended microcosms, the putative degraders were in the classDeltaproteobacteria, within the familySyntrophobacteraceae(digester sludge) orDesulfobulbaceae(contaminated soil) (genus unclassified for both). Partial benzylsuccinate synthase gene (bssA, the functional gene for anaerobic toluene degradation) sequences were obtained for some samples, and quantitative PCR targeting this gene, along with SIP, was further used to confirm anaerobic toluene degradation by the identified species. The study illustrates the diversity of toluene degraders across different environments and highlights the utility of ribosomal and functional gene-based SIP for linking function with identity in microbial communities.

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