Abstract
A bacterial lipase producer, designated SCS 2–3, was isolated from deep-sea sediment of the South China Sea. Phylogenetic analysis based on the 16S rRNA sequences revealed that strain SCS2-3 belonged to the genus Pseudomonas and had 98.56% similarity with P. xinjiangensis NRRL B-51270T that the closest relative strain. MLSA using four protein-coding genes (dnaK, gyrA, recA, and rpoB) showed strain SCS 2–3 to branch separately from its most closely related type strains. ANI and in silico DDH values between strain SCS 2–3 and the closely related type strains of Pseudomonas species were less than 81.51% and 23.80%, respectively, both below the species delineation threshold. Genome comparison showed that strain SCS 2–3 shared 1875 core gene families with other eight closely related type strains in Pseudomonas, and the number of strain-unique genes was 263. Through gene annotations, genes related to lipase was found in the isolates’ genomes. Furthermore, a combination of phenotypic, chemotaxonomic, phylogenetic and genotypic data clearly indicated that strain SCS 2–3 represents a novel species of the genus Pseudomonas, for which the name Pseudomonas nanhaiensis sp. nov. is proposed. The type strain is SCS 2-3T (= GDMCC 1.2219T = JCM 34440T).
Abyssomicins from the South China Sea Deep-Sea SedimentVerrucosisporasp.: Natural Thioether Michael Addition Adducts as Antitubercular Prodrugs
