Faculty Opinions recommendation of Centriole amplification by mother and daughter centrioles differs in multiciliated cells.

Saccharomyces cerevisiae cells select bud sites according to one of two predetermined patterns. MATa and MATα cells bud in an axial pattern, and MATa/α cells bud in a bipolar pattern. These budding patterns are thought to depend on the placement of spatial cues at specific sites in the cell cortex. Because cytoskeletal elements play a role in organizing the cytoplasm and establishing distinct plasma membrane domains, they are well suited for positioning bud-site selection cues. Indeed, the septin-containing neck filaments are crucial for establishing the axial budding pattern characteristic of MATa and MATα cells. In this study, we determined the budding patterns of cells carrying mutations in the actin gene or in genes encoding actin-associated proteins: MATa/α cells were defective in the bipolar budding pattern, but MATa and MATα cells still exhibit a normal axial budding pattern. We also observed that MATa/α actin cytoskeleton mutant daughter cells correctly position their first bud at the distal pole of the cell, but mother cells position their buds randomly. The actin cytoskeleton therefore functions in generation of the bipolar budding pattern and is required specifically for proper selection of bud sites in mother MATa/α cells. These observations and the results of double mutant studies support the conclusion that different rules govern bud-site selection in mother and daughter MATa/α cells. A defective bipolar budding pattern did not preclude an sla2-6 mutant from undergoing pseudohyphal growth, highlighting the central role of daughter cell bud-site selection cues in the formation of pseudohyphae. Finally, by examining the budding patterns of mad2-1 mitotic checkpoint mutants treated with benomyl to depolymerize their microtubules, we confirmed and extended previous evidence indicating that microtubules do not function in axial or bipolar bud-site selection.

Download Full-text

First evidence of Smith-Magenis syndrome in mother and daughter due to a novel RAI mutation

American Journal of Medical Genetics Part A ◽

10.1002/ajmg.a.37989 ◽

2016 ◽

Vol 173 (1) ◽

pp. 231-238 ◽

Cited By ~ 4

Author(s):

Fabio Acquaviva ◽

Maria Elena Sana ◽

Matteo Della Monica ◽

Michele Pinelli ◽

Diana Postorivo ◽

...

Keyword(s):

Mother And Daughter

Download Full-text

Building a ciliated epithelium: Transcriptional regulation and radial intercalation of multiciliated cells

10.1016/bs.ctdb.2020.08.001 ◽

2020 ◽

Author(s):

Caitlin Collins ◽

Rosa Ventrella ◽

Brian J. Mitchell

Keyword(s):

Transcriptional Regulation ◽

Ciliated Epithelium ◽

Multiciliated Cells

Download Full-text

Mother and daughter with a terminal Xp deletion: Implication of chromosomal mosaicism and X-inactivation in the high clinical variability of the microphthalmia with linear skin defects (MLS) syndrome

European Journal of Medical Genetics ◽

10.1016/j.ejmg.2007.07.004 ◽

2007 ◽

Vol 50 (6) ◽

pp. 421-431 ◽

Cited By ~ 12

Author(s):

Isabella Wimplinger ◽

Anita Rauch ◽

Ulrike Orth ◽

Ulrich Schwarzer ◽

Udo Trautmann ◽

...

Keyword(s):

X Inactivation ◽

Chromosomal Mosaicism ◽

Clinical Variability ◽

Skin Defects ◽

Mother And Daughter ◽

Xp Deletion

Download Full-text

Multiciliated cell basal bodies align in stereotypical patterns coordinated by the apical cytoskeleton

The Journal of Cell Biology ◽

10.1083/jcb.201601023 ◽

2016 ◽

Vol 214 (5) ◽

pp. 571-586 ◽

Cited By ~ 34

Author(s):

Elisa Herawati ◽

Daisuke Taniguchi ◽

Hatsuho Kanoh ◽

Kazuhiro Tateishi ◽

Shuji Ishihara ◽

...

Keyword(s):

Fluorescent Protein ◽

Imaging System ◽

Basal Bodies ◽

Large Numbers ◽

Multiciliated Cells ◽

Alignment Process ◽

Flow Through ◽

Linear Alignment ◽

Green Fluorescent

Multiciliated cells (MCCs) promote fluid flow through coordinated ciliary beating, which requires properly organized basal bodies (BBs). Airway MCCs have large numbers of BBs, which are uniformly oriented and, as we show here, align linearly. The mechanism for BB alignment is unexplored. To study this mechanism, we developed a long-term and high-resolution live-imaging system and used it to observe green fluorescent protein–centrin2–labeled BBs in cultured mouse tracheal MCCs. During MCC differentiation, the BB array adopted four stereotypical patterns, from a clustering “floret” pattern to the linear “alignment.” This alignment process was correlated with BB orientations, revealed by double immunostaining for BBs and their asymmetrically associated basal feet (BF). The BB alignment was disrupted by disturbing apical microtubules with nocodazole and by a BF-depleting Odf2 mutation. We constructed a theoretical model, which indicated that the apical cytoskeleton, acting like a viscoelastic fluid, provides a self-organizing mechanism in tracheal MCCs to align BBs linearly for mucociliary transport.

Download Full-text