Faculty Opinions recommendation of Bacterial vesicle secretion and the evolutionary origin of the eukaryotic endomembrane system.

International Allelopathy Society has redefined Allelopathy as any process involving secondary metabolities produced by plants, algae, bacteria, fungi and viruses that influences the growth and development of agricultural and biological system; a study of the functions of secondary metabolities, their significance in biological organization, their evolutionary origin and elucidation of the mechanisms involving plant-plant, plant-microorganisms, plant-virus, plant-insect, plant-soil-plant interactions.

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Identification of a Signal That Distinguishes between the Chloroplast Outer Envelope Membrane and the Endomembrane System in Vivo

The Plant Cell ◽

10.1105/tpc.13.10.2175 ◽

2001 ◽

Vol 13 (10) ◽

pp. 2175-2190 ◽

Cited By ~ 105

Author(s):

Y. J. Lee

Keyword(s):

Envelope Membrane ◽

Endomembrane System ◽

Outer Envelope ◽

Outer Envelope Membrane

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Stochastic activation and bistability in a Rab GTPase regulatory network

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1921027117 ◽

2020 ◽

Vol 117 (12) ◽

pp. 6540-6549

Author(s):

Urban Bezeljak ◽

Hrushikesh Loya ◽

Beata Kaczmarek ◽

Timothy E. Saunders ◽

Martin Loose

Keyword(s):

Regulatory Network ◽

Regulatory Networks ◽

Membrane Surface ◽

Activity Patterns ◽

Small Gtpases ◽

Signaling Networks ◽

Rab Gtpase ◽

Endomembrane System ◽

In Vitro Reconstitution

The eukaryotic endomembrane system is controlled by small GTPases of the Rab family, which are activated at defined times and locations in a switch-like manner. While this switch is well understood for an individual protein, how regulatory networks produce intracellular activity patterns is currently not known. Here, we combine in vitro reconstitution experiments with computational modeling to study a minimal Rab5 activation network. We find that the molecular interactions in this system give rise to a positive feedback and bistable collective switching of Rab5. Furthermore, we find that switching near the critical point is intrinsically stochastic and provide evidence that controlling the inactive population of Rab5 on the membrane can shape the network response. Notably, we demonstrate that collective switching can spread on the membrane surface as a traveling wave of Rab5 activation. Together, our findings reveal how biochemical signaling networks control vesicle trafficking pathways and how their nonequilibrium properties define the spatiotemporal organization of the cell.

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