Implementation of the method of acidic erythrograms to reveal the membranotropic effect of biologically active peptides

The method of acidic erythrograms was used to study the membranotropic effect of biologically active peptides (BAP). The air exposure of erythrocyte suspension in isotonic sodium chloride solution caused a decrease in their resistance to acid hemolysis: the time of hemolysis decreased, the integral indicator of erythrocyte resistance decreased too. The air exposure of erythrocyte suspension with the BAP solutions (10-8M) changed the hemolytic resistance of erythrocytes. It was found out that a non-opiate analogue of leu-enkephalin (NALE – Phe-D-Ala-Glu-Phe-Leu-Arg), Selank (Thr-Lys-Pro-Arg-ProGly-Pro) and Semax (Met-Glu -His-Phe-Pro-Gly-Pro) increase the resistance of erythrocyte membranes to acid hemolysis, and the Arg-Gly-Arg-Pro-Gly-Pro peptide decreases the resistance of erythrocyte membranes to acid hemolysis. Membranotropic action can determine the nonspecific effects of BAP and the range of their clinical use. Acidic erythrograms can be used as a screening method for the selection of BAP with membranotropic effects.