scholarly journals Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.

2021 ◽  
Vol 35 (3) ◽  
pp. 285-292
Author(s):  
Secil Kivrak Kiran ◽  
Selin Galatali ◽  
Sevil Yeniocak ◽  
Damla Ekin Ozkaya ◽  
Taner Mercan ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Corylus Avellana ◽  
Polymorphic Band ◽  
Meristem Culture ◽  
In Vitro Production ◽  
Northern Regions ◽  
Pcr Products ◽  
Production Area ◽  
Corylus Avellana L

Cultivation of Corylus avellana L. in Turkey is performed generally in the northern regions where it is an important source of livelihood for the local farmers. More than 70% of world hazelnut production is supplied by Turkey, but compared with other countries, Turkey’s hazelnut production area is quite narrow. In this study was aimed to develop an effective in vitro production for seven local cultivars of C. avellana. Therefore, WPM medium supplemented with 6-Benzylaminopurine (BAP) was modified by using single or in combination of Fe-EDDHA, AgNO3, H3BO3, charcoal and gibberellic acid. In all varieties, the best regeneration rates varying between 68% and 94% were obtained from WPM medium supplemented with 4.4 µM BAP, 27.8 µM Fe-EDDHA and 10g/L Charcoal. Genetic stability of shoots derived from meristem culture using the best medium was analysed using ISSR primers, when the gel images of the PCR products were examined, no polymorphic band was observed in samples collected from seven provinces, and the genetic stability was determined as 100%.

scholarly journals (288) Determination of Genetic Stability of In Vitro-derived Birch Plants

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1077C-1077
Author(s):  
Wenhao Dai ◽  
Victoria Magnusson ◽  
Andrea Swanberg
Keyword(s):  
Genetic Stability ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Fidelity ◽  
Genetic Variations ◽  
Meristem Culture ◽  
In Vitro Techniques ◽  
Somaclonal Variations ◽  
Regenerated Plants ◽  
Leaf Tissues

Many woody plants, including some birch species, can be cloned using such in vitro techniques as pre-existing meristem culture, organogenesis, and embryogenesis. However, clonal fidelity of in vitro-derived plants is always a big concern because somaclonal variations may be induced during the entire in vitro process. To address this issue, we used random amplified polymorphic DNA (RAPD) markers to determine the genetic stability of in vitro-propagated plants of Betula platyphylla `Fargo'. Forty-two greenhouse-grown birch plants derived from a 10-year shoot tip culture (shoot-derived) and 42 in vitro plants regenerated from leaf tissues (regenerated) were randomly selected and evaluated for their genetic fidelity by RAPD. To date, 20 primers (C1-C20, Operon Technologies) were screened for all 84 plants. Only strong bands that are conservative were scored. Each primer generated a unique set of amplification products. Most of scoreable bands are ranged from 350 to 1800 bp. A total of 3696 fragments were amplified from 42 shoot-derived plants by all 20 primers with an average of 4.4 bands per primer, in which 6 primers produced polymorphic bands, indicating some genetic variations within shoot-derived plants. Nineteen out of 20 primers yielded 2772 clear and reproducible bands (an average of 3.47 per primer) from 42 regenerated plants with no significant variations being detected. Our preliminary results showed that in vitro regenerated plants are genetically uniform. However, a long-term tissue culture might result in a few genetic variations of birch species.

In vitro Production of Pathogen-free Plantlets via Meristem Culture in Saffron (Crocus sativus L.)

2006 ◽  
Vol 5 (3) ◽  
pp. 292-295 ◽  
Author(s):  
E. Darvishi . ◽  
R. Zarghami . ◽  
C.A. Mishani . ◽  
M. Omidi . ◽  
A. Sarkhosh .
Keyword(s):  
Crocus Sativus ◽  
Meristem Culture ◽  
In Vitro Production ◽  
Vitro Production ◽  
Crocus Sativus L

?In vitro? filbert (Corylus avellana L.) micropropagation from shoot and cotyledonary node segments

1985 ◽  
Vol 4 (3) ◽  
pp. 137-139 ◽  
Author(s):  
C�sar P�rez ◽  
Roberto Rodr�guez ◽  
Ricardo S. Tam�s
Keyword(s):  
Cotyledonary Node ◽  
Corylus Avellana ◽  
Corylus Avellana L

scholarly journals Multiplication in vitro du noisetier (Corylus avellana L.)

Agronomie ◽  
1984 ◽  
Vol 4 (4) ◽  
pp. 399-402 ◽  
Author(s):  
Hanan AL KAÏ ◽  
Georges SALESSES ◽  
Armand MOURAS
Keyword(s):  
Corylus Avellana ◽  
Corylus Avellana L

In vitro rooting of hybrid hazelnuts (Corylus avellana × Corylus americana) in a temporary immersion system

Botany ◽  
2020 ◽  
Vol 98 (7) ◽  
pp. 343-352 ◽  
Author(s):  
James Nicholson ◽  
Mukund R. Shukla ◽  
Praveen K. Saxena
Keyword(s):  
Cost Effective ◽  
Corylus Avellana ◽  
In Vitro Rooting ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion System ◽  
Shoot Height ◽  
Temporary Immersion ◽  
Corylus Avellana L ◽  
Commercial Micropropagation

Commercial micropropagation of hybrid hazelnuts (Corylus avellana L. × C. americana Marshall) has been limited, owing to their poor rooting ability in vitro as well as ex vitro, leading to high mortality of plantlets transplanted in the greenhouse. The objective of this study was to develop an efficient and cost-effective protocol for rooting and plantlet acclimation of in vitro grown hazelnut shoots. Efficient in vitro rooting was accomplished in a rocker-based temporary immersion bioreactor system. The use of a temporary immersion system (TIS) in combination with the inert substrate Oasis® In Vitro Express (IVE) significantly improved the in vitro rooting efficiency (100%) compared with semi-solid medium (27%) after four weeks of culture. A higher density (36 explants/vessel) of shoot explants in the TIS was found to support a significantly greater shoot height, chlorophyll content, and longest root length, compared with the lowest density treatment (12 explants/vessel). Efficiency of rooting and the number of roots formed were similar for both the high and low density of explants in the culture vessels, and the resulting plantlets exhibited > 80% survival in the greenhouse. These results demonstrate the usefulness of rocker-based TIS for commercial micropropagation of hazelnuts and, potentially, other tree species.

Exogenous Polyamine Application Improves Shoot Elongation In Hazelnut (Corylus avellana L.) Micropropagation

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 462d-462
Author(s):  
Mehmet Nuri Nas ◽  
Paul E. Read
Keyword(s):  
Shoot Elongation ◽  
Corylus Avellana ◽  
Corylus Avellana L ◽  
Different Levels

Buds from newly developed shoots obtained from forced outgrowth of mature, unpruned field-grown trees of Corylus avellana L. were cultured in vitro on Murashige and Skoog (1962) medium and a Modified Driver and Kuniyuki (1984) medium containing different levels of N-6 Benzyladenine (BA) (1.5, 2.5, or 3.5 mg/L) supplemented with or without a combination of Putrescine (0.2 mM) + Spermidine (0.2 mM) + Spermine (0.05 mM). Shoot elongation varied among genotypes, media and treatment with/or without polyamines. The best shoot elongation occurred on modified DKW medium containing BA plus polyamines.

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