Determination of Ankyrin Repeats Domain (ANK) a of RNASE L Gene in Hepatitis C Patients and its effects on Viral Load

Pakistan Journal of Medicine and Dentistry ◽

10.36283/pjmd9-3/002 ◽

2020 ◽

Author(s):

Anum Liaquat Ali

Keyword(s):

Viral Load ◽

Hepatitis C ◽

Pcr Amplification ◽

Ankyrin Repeat ◽

Hcv Infection ◽

Rnase L ◽

Ankyrin Repeats ◽

Genotype 3 ◽

Repeat Domain ◽

Ankyrin Repeat Domain

Background: Hepatitis C virus (HCV) is one of the major global causes of death. Different types of gene are involved as Ankyrin repeat domains of RNASE L gene. It performs a significant role in antiviral response, regulated by interferon, and involved in cleavage of RNA. Therefore, aim of this study was to identify Ankyrin repeat domain expression in Hepatitis C positive patients and correlate it with viral load of Hepatitis C. Methods: In this study, a total of 80 HCV positive patient’s whole blood samples were investigated. RNA was extracted from plasma followed by Real Time PCR for quantization of HCV viral load and genotypic analyses. DNA was also extracted from these samples followed by PCR amplification of Ankyrin repeat domain of RNASE L gene. Data was analyzed using SPSS Results: All of the patients (n=80) included in study had HCV infection. Mean age of patients was 50.86 ±14.84 years. Among them, 48(63.8%) were males and 32 (36.1%) were females. Majority of patients were males and belonged to age group 58-73 years age. All HCV infected individuals 36 (45%) had HCV genotype 3 and had viral loads mean range 837404.21 ±1302. Therefore, Ankyrin repeats domain of RNASE L gene expression was high in HCV patients sample with viral load of 17.00±15.1. Conclusion: Ankyrin repeat domain expression was observed in Hepatitis C patients and its significant correlation with viral load of Hepatitis C. Ankyrin repeat domain of RNASE L gene in conjunction with therapeutic intervention are required for establishing better strategies for controlling HCV infection.

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Prevalence of Hepatitis C Infection and its Genotypes in Suspected Hemodialysis Patients, Southwest of Iran

Jundishapur Journal of Microbiology ◽

10.5812/jjm.118591 ◽

2021 ◽

Vol 14 (11) ◽

Author(s):

Marzieh Jamalidoust ◽

Maryam Eskandari ◽

Mazyar Ziyaeyan

Keyword(s):

Viral Load ◽

Hepatitis C ◽

Management Practices ◽

Hemodialysis Patients ◽

Hcv Infection ◽

Research Center ◽

Enzyme Linked Immunosorbent Assay ◽

Hcv Rna ◽

Hepatitis C Infection ◽

Genotype 3

Background: Hemodialysis patients are more prone to Hepatitis C Virus (HCV) infection due to the need for long-term hemodialysis and blood transfusions. Objectives: The present study aimed to determine the HCV infection burden, viral load, and genotype pattern in hemodialysis patients referred to a research center from 2011 to 2018. Methods: Among 131 hemodialysis patients with suspected HCV infection, referred to Prof. Alborzi Clinical Microbiology Research Center, Shiraz, Iran, from 2011 to 2018, the HCV rate was assessed with the enzyme-linked immunosorbent assay and the HCV RNA load and genotypes by one-step TaqMan real-time PCR. Results: The prevalence of HCV-Ab positivity was 29% among hemodialysis patients, of whom 21 (57%) were HCV RNA-positive. In the rest of the hemodialysis patients who were HCV-Ab-negative, the HCV RNA was detected in five (12%) patients. Genotype 3 (Gt-3) was the most prevalent one detected in 50% of the patients whose genotypes were determined. Also, the HCV viral load in HCV-seropositive patients was generally higher than that in HCV-seronegative ones. Conclusions: This study showed that high HCV infection and different genotype patterns among hemodialysis patients compared to the general population are the main predictors of HCV infection, which indicates healthcare facility transmission because of inappropriate infection management practices.

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Clinical, Biochemical and Virological Profile of Patients with Chronic Hepatitis C Virus Infection - A Study from University Hospital in Nepal

Journal of Nobel Medical College ◽

10.3126/jonmc.v4i1.13301 ◽

2015 ◽

Vol 4 (1) ◽

pp. 32-35

Author(s):

Dipesh Gurubacharya ◽

Mohan Khadka ◽

Khadga B Shreshta ◽

Prem Khadga ◽

Sashi Sharma

Keyword(s):

Hepatitis C Virus ◽

Viral Load ◽

Hepatitis C ◽

Drug Use ◽

Injection Drug Use ◽

Hcv Infection ◽

Genotype 3 ◽

Hcv Genotypes ◽

Chronic Hcv Infection ◽

Chronic Hcv

Introduction: Hepatitis C virus (HCV) infection is a major public health challenge. It is a major cause for cirrhosis and hepatocellular carcinoma worldwide. Both the genotype and viral load of HCV determine the choice of therapy as well as outcome of therapy. The aim of this study was to evaluate clinical, biochemical and virological profile and association of HCV genotypes with viral load and liver biochemical profile.Material and Methods: This was descriptive observational study of chronic HCV infected patients who attended at the outpatient clinic of Department of Gastroenterology of TUTH, IOM from April 2013 to November 2014. During this study period 38 patients with chronic HCV infection were analyzed. Clinical profile, possible risk factors for transmission of HCV infection and liver biochemical profile were recorded. Virological profile included HCV viral load and HCV genotypes.Results: Out of 38 patients 34(89.5%) were male and 4(10.5%) were female. Injection drug use (IDU) was the most common mode for acquisition of HCV infection (55.3%). Genotype 3 was found in 21(55.26%) patients and genotype 1 was found in 17(44.74%) patients. There was no significant association between HCV genotypes and serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) level. And also there was no significant association between HCV viral load and different HCV genotypes.Conclusions: In our study HCV genotype 3 was the most prevalent genotype in patients with chronic HCV infection. Injection drug use was identified as most common identifiable risk factor for transmission of HCV infection. There was no significant association between different HCV genotypes and serum ALT, AST level and HCV viral load. Journal of Nobel College of Medicine Vol.4(1) 2015: 32-35

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Clinical and epidemiological characteristics and gene variant structure of HCV-infection in the area of the Krasnodar Territory

Epidemiology and Infectious Diseases (Russian Journal) ◽

10.17816/eid40761 ◽

2013 ◽

Vol 18 (6) ◽

pp. 8-16

Author(s):

M. G. Avdeeva ◽

V. N. Gorodin ◽

A. A. Konchakova ◽

V. A. Dubinina ◽

N. V. Kotova ◽

...

Keyword(s):

Viral Load ◽

Hepatitis C ◽

Clinical Manifestations ◽

Hcv Infection ◽

Genotype 3 ◽

Virus Genotype ◽

Hcv Genotypes ◽

Genotype 1B ◽

Genotype 2 ◽

Epidemiological Characteristics

The purpose of research - based on analysis of the clinical-epidemiologic data, the level of viral load and the prevalence of HCV genotypes to determine current consistencies and epidemiological trends of the development of HCV-infection in the area of the Krasnodar Territory. Patients and methods. Population incidence of hepatitis C in the Krasnodar Territory was studied for the period from 2004 to 2011. Clinical manifestations of the disease we evaluated on the base of a retrospective analysis of 1913 hospital records of patients with chronic hepatitis C. There were investigated the results of 19,338 qualitative PCR studies of RNA-HCV, 2041 studies for genotyping and 363 results of quantitative evaluation of RNA-HCV Results. In the Krasnodar Territory there is revealed circulation of 1a, 1b, 2, 3a HCV genotypes. In a population of genotypes 1b (48 %) and 3a (38 %) prevail. In recent years there has been visible trend to displacement of genotype 1b by the increase the incidence of genotype 3 a in a set of areas. Social Portrait of the patient with hepatitis C: a man aged 28 to 48, a city resident, a salary earner or unemployed. There were established gender peculiarities: males are more often infected with hepatitis C genotype 3a, females - genotype 1b. In most cases (65%) viremia is characterized by moderate viral load. In the studied population there is revealed the relationship between the activity of the process and infection with certain genotype. Moderate grade of hepatitis activity is significantly more frequently recorded in the disease caused by 1c and 3 a virus subtypes. Minimal activity of hepatitis is not typical for the process caused by genotype 2 and in genotype 3 a occurs much less frequently than in cases caused by genotype 1b. Conclusion. Epidemiological analysis of the incidence of hepatitis C should take into account trends in the spread of various genotypes of the virus. Hepatitis C virus genotype should be taken into account as in the forecast of the disease and in determination of the indications for antiviral therapy. The main socio-age group actively involved in the epidemic process and need for causal treatment, are young persons of working age.

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Crystallization of the N-Terminal Ankyrin Repeat Domain of the 2-5ADependent Endoribonuclease, RNase L

Protein and Peptide Letters ◽

10.2174/0929866053765734 ◽

2005 ◽

Vol 12 (4) ◽

pp. 387-389 ◽

Cited By ~ 3

Author(s):

Nobutada Tanaka ◽

Masayuki Nakanishi ◽

Yoshio Kusakabe ◽

Yoshikuni Goto ◽

Yukio Kitade ◽

...

Keyword(s):

Ankyrin Repeat ◽

Rnase L ◽

Repeat Domain ◽

Ankyrin Repeat Domain

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2-5A induces a conformational change in the ankyrin-repeat domain of RNase L

Proteins Structure Function and Bioinformatics ◽

10.1002/prot.20474 ◽

2005 ◽

Vol 60 (1) ◽

pp. 131-138 ◽

Cited By ~ 20

Author(s):

Masayuki Nakanishi ◽

Yoshikuni Goto ◽

Yukio Kitade

Keyword(s):

Conformational Change ◽

Ankyrin Repeat ◽

Rnase L ◽

Repeat Domain ◽

Ankyrin Repeat Domain

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Crystal structure of the N-terminal ankyrin repeat domain of human RNase L

Acta Crystallographica Section A Foundations of Crystallography ◽

10.1107/s0108767305089440 ◽

2005 ◽

Vol 61 (a1) ◽

pp. c247-c248

Author(s):

N. Tanaka ◽

M. Nakanishi ◽

Y. Kusakabe ◽

Y. Goto ◽

Y. Kitade ◽

...

Keyword(s):

Crystal Structure ◽

Ankyrin Repeat ◽

Rnase L ◽

Human Rnase ◽

Repeat Domain ◽

Ankyrin Repeat Domain

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Comparative analysis of the interleukin-28b gene alleles in patients with hepatitis C monoinfection and combined HIV/hepatitis C infection

Kazan medical journal ◽

10.17816/kmj2177 ◽

2013 ◽

Vol 94 (3) ◽

pp. 316-320

Author(s):

V K Fazylov ◽

E R Manapova ◽

S V Tkacheva ◽

F M Yakupova ◽

A T Beshimov

Keyword(s):

Viral Load ◽

Comparative Analysis ◽

Hepatitis C ◽

Absolute Number ◽

Hcv Infection ◽

Hcv Rna ◽

Hepatitis C Infection ◽

Genotype 3 ◽

Median Percentage ◽

Baseline Activity

Aim. To identify the frequency and to perform the comparative analysis of IL-28B genotypes distribution in patients with hepatitis C (HCV) mono-infection and combined HIV/HCV infection. Methods. 101 patients (65% - males, average age 33.85±0.62 years) were included in the study. The first group (n=58) - patients with HCV mono-infection, second (n=43) - patients with combined HIV/HCV infection. Single nucleotides polymorphism at rs8099917 and rs12979860 locuses of IL-28B gene was performed using the «AmpliSens Genoscreen-IL28V-FL» reagent kit in the viral hepatitis laboratory of the molecular diagnostics department, CSRIE, Moscow. The baseline activity of HCV infection was determined by RNA-HCV viral load 400 000 copies/mL and alanine aminotransferase level (ALT). In the first group high RNA-HCV viral load (400 000 copies/mL) was observed in 30 (51.7%) patients, mean ALT level was 72.79±9.85 U/L; in the second group high HCV-RNA viral load was detected in 35 (81.4%) patients, mean ALT level was 85.46±7.73 U/L. In the group with combined infection 26 (60.5%) of patients received antiretroviral therapy (ART); in 14 (53.8%) of them there was no detectable viral load. The median absolute number of CD4+ lymphocytes was 0.400±0.04 cells/μL, median percentage - 25.41%±2.41. In 17 (39.5%) treatment-naïve patients the viral load was low (10 000 copies/mL), in 11 (64.8%) of these patients mean CD4+ count was 0.470±0.04 cells/μL (25.33%±2.15); the term of HIV antibodies detection was 6.89±0.53 years. The sensitivity of the PCR method for the qualitative detection of HCV-RNA was 111.1 copies/mL, quantitative - 275 copies/mL, for HIV-RNA - 150 copies/mL. Results. In patients with HCV mono-infection the rate of unfavorable IL-28B CT and TT rs12979860 genotypes and ТG and GG rs8099917 genotypes was 45 (77.6%) и 26 (44.8%) correspondingly, favorable СС rs12979860 and ТТ rs8099917 genotypes were registered in 13 (22.4%) and 32 (55.1%) patients correspondingly. In patients with HIV/HCV infection (n=43) unfavorable IL-28B CT and TT rs12979860 genotypes and ТG and GG rs8099917 genotypes were detected in 20 (46.5%) и 13 (30.2%) of cases, and favorable СС rs12979860 and ТТ rs8099917 genotypes - in 23 (53.5%) and 30 (69.8%) of cases correspondingly. Conclusion. Results show that favorable CC rs12979860 and TT rs8099917 genotypes of the IL-28B and their combination are found quite frequently in patients with mixed HCV/HIV infection, particularly in patients with HCV genotype 3, these patients also had more significant inflammatory reaction and high HCV RNA viral load compared to HCV mono-infected patients.

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