Dynamics of basophil reactivity in the patients with allergic rhinitis on the background of allergen-specific immunotherapy

BACKGROUND: Allergen-specific immunotherapy (ASIT) helps to reduce spontaneous and to induce basophil reactivity, which is one of the major mechanisms of its effectiveness. A number of studies, though, have not confirmed the informative value of the basophil activation test (BAT), in case of ASIT, with house dust mites, meadow grass pollen, and wasp venom. BAT outcomes are likely to be influenced by the properties of the sensitizing allergen, the duration of ASIT, and the BAT markers that are used. AIMS: To evaluate the basophils reactivity in the patients sensitized to weed pollen and treated with allergen-specific therapy. MATERIALS AND METHODS: The study involved 60 patients (aged 1655) with allergic rhinitis sensitized to weed pollen. The diagnosis was based on the skin test outcomes as well as on identifying specific antibodies to major allergens of ragweed, wormwood, timothy, and birch. The pre-seasonal ASIT involved water-salted extracts of pollen allergens during 2 years. Clinical symptoms and the immunotherapy effectiveness were evaluated 6 and 18 months after the ASIT started. BATs were made before the treatment, after the first and second courses of pre-seasonal ASIT, which corresponded to zero, the 3rd and the 15th months of the treatment. The activated basophils were identified based on the CRTH2pos CD203brightCD3neg phenotype. RESULTS: The first treatment course was completed by 52 patients, while the second one by 43 patients. A statistically significant decrease in spontaneous basophil reactivity was detected only after the second course of ASIT. The decrease in the allergen-induced basophil activation was identified in 67.4% of the samples obtained after the first ASIT course, and in 67.6% of samples taken after the second immunotherapy course. A decrease in the BAT stimulation index (BAT SI) was identified mainly in the patients featuring positive treatment outcomes. CONCLUSIONS: ASIT influences on the basophil reactivity indicators in the patients sensitized to weed pollen. The basophil activation test may be considered as a potential predictor of ASIT effectiveness.

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Optimization of the basophil activation test in the diagnosis and qualification for treatment by means of allergen-specific immunotherapy in children with respiratory allergy to house dust mites

Clinical and Translational Allergy ◽

10.1186/2045-7022-5-s1-p10 ◽

2015 ◽

Vol 5 (Suppl 1) ◽

pp. P10

Author(s):

Radoslaw Spiewak ◽

Aleksandra Gregorius ◽

Ewa Czarnobilska

Keyword(s):

House Dust ◽

Specific Immunotherapy ◽

Basophil Activation Test ◽

Respiratory Allergy ◽

Basophil Activation ◽

House Dust Mites ◽

Dust Mites ◽

Allergen Specific Immunotherapy ◽

Activation Test

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The clinical and biological response to allergen-specific immunotherapy with basophil activation test (BAT) in the atopic diseases

10.1183/13993003.congress-2015.pa4019 ◽

2015 ◽

Author(s):

Anna Agnese Stanziola ◽

Giovanna Caccavo ◽

Maurizia Lanza ◽

Sara Carputo ◽

Maria Martino ◽

...

Keyword(s):

Specific Immunotherapy ◽

Biological Response ◽

Basophil Activation Test ◽

Basophil Activation ◽

Atopic Diseases ◽

Allergen Specific Immunotherapy ◽

Activation Test

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Analysis of the dynamics of clinical indicators in patients with allergic rhinitis with sensitization to pollen and household allergens using combined allergen-specific immunotherapy

Medicni perspektivi (Medical perspectives) ◽

10.26641/2307-0404.2021.4.248161 ◽

2021 ◽

Vol 26 (4) ◽

pp. 87-93

Author(s):

S.V. Biletska ◽

E.M. Dytyatkovska ◽

M.A. Nikolaychuk

Keyword(s):

Allergic Rhinitis ◽

Specific Immunotherapy ◽

High Efficiency ◽

Comparison Group ◽

Clinical Symptoms ◽

Clinical Manifestations ◽

House Dust Mites ◽

Skin Tests ◽

Pollen Allergens ◽

Allergen Specific Immunotherapy

The aim of this study was to evaluate the clinical efficacy of combined allergen-specific immunotherapy (ASIT) in patients with allergic rhinitis (AR) with combined sensitization to pollen and household allergens. To achieve this goal, 49 patients with AR of working age were examined – 35.5±1.5 years with clinical manifestations of seasonal rhinoconjunctival syndrome with a long period of 9.2±1.1 years, among which there were 25 (51.0%) males and 24 (49%) females. All patients were divided into 2 homogeneous groups by age, sex, duration of the disease, the average number of etiologically significant allergens: the main one – 31 patients who received combined ASIT with solutions of pollen and household allergens and a comparison group – 18 patients for whom only pollen allergens were used. Allergological examination included anamnesis, skin tests with pollen allergens (wormwood, ragweed, quinoa, corn, etc.) and household (house dust, mites, epidermal agents) and / or molecular research methods using the ALEX technology. The quantitative integral assessment of the intensity of AR clinical symptoms was calculated as a total score for the main symptoms. The maximum score for the severity of nasal symptoms – 12, eye - 6, total – 18. The results obtained and their analysis indicate that under the influence of ASIT pollen and household allergens in patients there is a significant and reliable decrease in the intensity of clinical manifestations of seasonal rhinoconjunctive syndrome: nasal manifestations – by 52,2%, conjunctival – by 60%, integral – by 54.3% and an increase of 2.2 times in the percentage of patients in the main group with the disappearance or minimization of clinical symptoms of the disease after treatment compared with patients from the comparison group, which convincingly proves and confirms high efficiency of the selected type of therapy in patients with AR in combination with sensitization to pollen and household allergens.

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BASOPHILE ACTIVATION TEST FOR THE DIAGNOSTICS OF FUNGAL SENSITIZATION IN THE PATIENTS WITH CYSTIC FIBROSIS

Medical Immunology (Russia) ◽

10.15789/1563-0625-2019-5-919-928 ◽

2019 ◽

Vol 21 (5) ◽

pp. 919-928

Author(s):

Ya. I. Kozlova ◽

E. V. Frolova ◽

A. E. Uchevatkina ◽

L. V. Filippova ◽

O. V. Aak ◽

...

Keyword(s):

Cystic Fibrosis ◽

Stimulation Index ◽

Allergic Bronchopulmonary Aspergillosis ◽

Basophil Activation Test ◽

Specific Ige ◽

Basophil Activation ◽

Total Ige ◽

Activation Test ◽

Fungal Allergens ◽

Fungal Sensitization

Aspergillus fumigatus colonization in the patients with cystic fibrosis (CF) may cause sensitization against A. fumigatus and/or allergic bronchopulmonary aspergillosis (ABPA), which significantly worsens the course of underlying disease. At the present time, new diagnostic tests are searched for detection of fungal sensitization in these patients. The aim of this work was to evaluate an opportunity of application of basophile activation test with A. fumigatus allergen in vitro using flow cytometry, aiming for identification of fungal sensitization in the CF patients. The study included 190 patients with CF aged 1 to 37 years. All the patients underwent common allergy screening (skin tests with fungal allergens, determination of serum levels of total IgE and specific IgE for the fungal allergens), and mycological examination (microscopy and culture of respiratory substrates). Computed tomography of the chest was performed upon clinical indications. The basophil activation test with the A. fumigatus allergen was performed in 10 CF patients with ABPA, and 10 CF patients without ABPA, in addition to the standard allergological examination. Frequency of sensitization to A. fumigatus in the patients with cystic fibrosis was 27%, the incidence of allergic bronchopulmonary aspergillosis was 5.7%. The number of eosinophils, total IgE and specific IgE levels in CF patients with ABPA were significantly higher than in CF patients without ABPA. In blood of the ABPA patients we have identified 68.5 (52.5-81.5%) of basophilic leukocytes activated by A. fumigatus allergen, with a stimulation index of 17.07 (10.30-27.70). In appropriate comparison group, the stimulation index did not exceed 1.5 (p = 0.000). Direct positive correlation between the levels of specific IgE to A. fumigatus and the number of basophils activated by A. fumigatus allergens was revealed (r = 0.77; р < 0.05). FVC values and the body mass index in CF patients with ABPA were significantly lower when compared with the patients without fungal sensitization. Introduction of the basophil activation test, along with standard techniques, may enable a more differentiated assessment of ABPA development in CF patients. Timely detection of associations between A. fumigatus sensitization and clinical status of CF patients will facilitate early and effective administration of specific therapy.

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Role of the basophil activation test in the diagnosis of local allergic rhinitis

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2013.07.016 ◽

2013 ◽

Vol 132 (4) ◽

pp. 975-976.e5 ◽

Cited By ~ 44

Author(s):

Enrique Gómez ◽

Paloma Campo ◽

Carmen Rondón ◽

Esther Barrionuevo ◽

Natalia Blanca-López ◽

...

Keyword(s):

Allergic Rhinitis ◽

Basophil Activation Test ◽

Basophil Activation ◽

Activation Test ◽

Local Allergic Rhinitis

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Clinical and allergological characteristic of children with allergic rhinitis receiving allergen-specific immunotherapy in Altai region

Rossiiskii Allergologicheskii ZHurnal ◽

10.36691/rja468 ◽

2015 ◽

Vol 12 (2) ◽

pp. 64-67

Author(s):

N V Shakhova ◽

V V Gordeev ◽

U F Lobanov ◽

T S Ardatova ◽

M V Surcova

Keyword(s):

Allergic Rhinitis ◽

Medical Record ◽

Allergic Conjunctivitis ◽

Specific Immunotherapy ◽

Primary Diagnosis ◽

House Dust Mites ◽

Allergen Specific Immunotherapy ◽

Altai Region ◽

Persistent Allergic Rhinitis ◽

Record Card

The purpose of this study. To investigate clinical characteristics of the allergic rhinitis in children receiving allergen-specific immunotherapy. Methods. The epidemiologic observational multicenter study was conducted with the allergists of Altai region. The participating allergists filled out the specially designed medical record card on children with allergic rhinitis receiving ASIT. The study lasted from October 2013 to March 2014. Results. Allergists have filled out medical record cards of 165 children with allergic rhinitis receiving ASIT. The average age of children was 10,5±2,3 years, with the average of 4,8±2,5 years of allergic rhinitis prior to the start of ASIT. 159 (96,2%) children had moderate/severe allergic rhinitis, and 121 (73,3%) children had persistent allergic rhinitis. In 123 (74,5%) children rhinitis was accompanied by allergic conjunctivitis, and in 70 (42,2%) children - by bronchial asthma. 69 (41,8%) children receiving ASIT were mono-sensitized, 96 (58,2%) children were polysensitized. 48,5% of children were sensitized to house dust mites, 63,6% to tree pollen, 48,5% to meadow grass pollen, 30,9% to weed pollen, and in 21,8% sensitization to epidermal allergens was found. Conclusion. The findings of the study showed that in most cases (96,2%), children with allergic rhinitis receiving ASIT had moderate/severe rhinitis and in 73,3% symptoms were persistent. In 74,5% of cases, allergic rhinitis was accompanied by allergic conjunctivitis. Most part of children receiving ASIT had multiple sensitization (58,2%). Also a delayed beginning of ASIT was observed - 4,8±2,5 year from primary diagnosis to ASIT initiation.

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