Molecular Cloning and Heterologous Expression of Putative (S)-norcoclaurine Synthases from Arabidopsis thaliana

2013 ◽  
Vol 19 (1) ◽  
pp. 61-68
Author(s):  
Xiang ZHANG ◽  
Lei MA ◽  
Yongqiang TIAN ◽  
Guolin ZHANG ◽  
Yinggang LUO
Keyword(s):  
Arabidopsis Thaliana ◽  
Heterologous Expression ◽  
Molecular Cloning

scholarly journals Molecular basis of N-acetylglucosaminyltransferase I deficiency in Arabidopsis thaliana plants lacking complex N-glycans

2005 ◽  
Vol 387 (2) ◽  
pp. 385-391 ◽  
Author(s):  
Richard STRASSER ◽  
Johannes STADLMANN ◽  
Barbara SVOBODA ◽  
Friedrich ALTMANN ◽  
Josef GLÖSSL ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Amino Acid ◽  
Heterologous Expression ◽  
Molecular Cloning ◽  
Molecular Basis ◽  
Insect Cells ◽  
Glycosylation Site ◽  
Enzymic Activity ◽  
Critical Amino Acid ◽  
High Mannose

GnTI (N-acetylglucosaminyltransferase I) is a Golgi-resident enzyme essential for the processing of high-mannose to hybrid and complex N-glycans. The Arabidopsis thaliana cgl mutant lacks GnTI activity and as a consequence accumulates oligomannosidic structures. Molecular cloning of cgl GnTI cDNA revealed a point mutation, which causes a critical amino acid substitution (Asp144→Asn), thereby creating an additional N-glycosylation site. Heterologous expression of cgl GnTI in insect cells confirmed its lack of activity and the use of the N-glycosylation site. Remarkably, introduction of the Asp144→Asn mutation into rabbit GnTI, which does not result in the formation of a new N-glycosylation site, led to a protein with strongly reduced, but still detectable enzymic activity. Expression of Asn144 rabbit GnTI in cgl plants could partially restore complex N-glycan formation. These results indicate that the complete deficiency of GnTI activity in cgl plants is mainly due to the additional N-glycan, which appears to interfere with the proper folding of the enzyme.

Molecular cloning of a laccase gene from Ganoderma lucidum and heterologous expression in Pichia pastoris

2013 ◽  
Vol 54 (S1) ◽  
pp. S134-S141 ◽  
Author(s):  
Lin-Feng You ◽  
Zhi-Ming Liu ◽  
Jun-Fang Lin ◽  
Li-Qiong Guo ◽  
Xun-Liu Huang ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Heterologous Expression ◽  
Molecular Cloning ◽  
Ganoderma Lucidum ◽  
Laccase Gene

Molecular Cloning and Characterization of a Calmodulin Gene from Arabidopsis thaliana

1994 ◽  
Vol 3 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Amaresh Chandra ◽  
M. Thungapathra ◽  
Kallash C. Upadhyaya
Keyword(s):  
Arabidopsis Thaliana ◽  
Molecular Cloning ◽  
Calmodulin Gene

scholarly journals Molecular cloning, heterologous expression, and functional characterization of a cellulolytic enzyme (Cel PRII) from buffalo rumen metagenome

3 Biotech ◽  
2017 ◽  
Vol 7 (4) ◽  
Author(s):  
Ravi K. Shah ◽  
Amrutlal K. Patel ◽  
Deepti M. Davla ◽  
Ishan K. Parikh ◽  
Ramalingam B. Subramanian ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Molecular Cloning ◽  
Functional Characterization ◽  
Cellulolytic Enzyme ◽  
Rumen Metagenome ◽  
Buffalo Rumen

Tryparedoxin Peroxidase of Leishmania donovani: Molecular Cloning, Heterologous Expression, Specificity, and Catalytic Mechanism

2002 ◽  
Vol 397 (2) ◽  
pp. 324-335 ◽  
Author(s):  
Leopold Flohé ◽  
Heike Budde ◽  
Karsten Bruns ◽  
Helena Castro ◽  
Joachim Clos ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Molecular Cloning ◽  
Leishmania Donovani ◽  
Catalytic Mechanism ◽  
Tryparedoxin Peroxidase

Molecular cloning and heterologous expression of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus x media

2006 ◽  
Vol 34 (2) ◽  
pp. 89-95 ◽  
Author(s):  
Binhui Guo ◽  
Guoyin Kai ◽  
Yifu Gong ◽  
Hongbin Jin ◽  
Yechun Wang ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Molecular Cloning ◽  
Taxus X Media
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