Cellulolytic and mannanolytic aerobic bacteria isolated from Buffalo rumen (Bubalus babalis) and its potency to degrade fiber in palm kernel meal

Sari SLA, Triyanto T, Zuprifal Z, Prijambada ID. 2021. Cellulolytic and mannanolytic aerobic bacteria isolated from Buffalo rumen (Bubalus babalis) and its potency to degrade fiber in palm kernel meal. Biodiversitas 22: 2829-2837. Palm kernel meal (PKM) is potential to be used as feed, but its high fiber content causes PKM meal difficult to be digested by monogastric animals. Ruminants are especially effective in digesting plant fibers because of the presence of microbes in their rumens. Based on those facts, this research was conducted to obtain mannanolytic and cellulolytic bacteria from buffalo rumens (Bubalus babalis, Linnaeus, 1758), which can degrade fibers in PKM. Bacteria were isolated from buffalo rumen by using PKM- isolation media. Screening of hydrolytic activities was done based on clear zone formation on screening media. A total of five bacterial isolates with the highest hydrolytic activities were then assayed quantitatively for their abilities to degrade mannan and cellulose, then identified based on 16S rRNA gene sequences. This research successfully isolated 34 bacterial isolates. The screening result demonstrated that all isolates could hydrolyze mannan, cellulose and polysaccharide in PKM. Isolate BR25 showed the highest hydrolytic ability on PKM and mannan screening media with clear zone diameter/colony diameter ratio (dz/dc ratio) of 2.99 and 3.53, respectively. Isolate BR31 showed the highest cellulolytic ability with dz/dc ratio value of 2.22. Five isolates with the highest hydrolytic activity, i.e. BR14, BR16, BR23, BR25, and BR30 showed the ability to grow on submerged media which contain locust bean gum (LBG) and microcrystalline cellulose (MCC) respectively, as single carbon source and isolate BR25 showed the highest ability to degrade mannan and cellulose. Based on the gene sequence of 16S rRNA, isolates BR14, BR16, BR23, BR25, and BR30 were identified to be closely related to Exiguobacterium acetylicum, Bacillus cereus, Klebsiella quasipneumoniae, Paenibacillus polymyxa, and Acinetobacter baumannii with 98.57-100% level of similarity.

The identification of fungi colonies total on the rumen content of cow and buffalo with addition of leaves and oil palm frond

This research aimed to determine the fungi colonies total on the rumen content of cattle and buffaloes with the addition of leaves and oil palm fronds incubated for 7 days. Each of the contents of the rumen was added with molasses, soybean soaking water as an energy source for microorganisms, as well as the addition of oil palm fronds and palm leaves to expect the type of fungus that is expected to grow. Calculation of the total colonies fungi in the rumen contents was carried out using total plate count with the dilution method. Complete Random Design by Factorial used to analyze the statistic data. Factor A was the type of rumen content (A1 = rumen of cattle, A2 = buffalo rumen), factor B was the addition of palm fronds (B1 = only rumen contents, B2 = palm oil fronds, B3 = palm leaves, and B4 = palm oil fronds and palm leaves). The results showed that the total number of fungal colonies was significantly higher in the rumen contents of cattle with an average of 509.17 104 CFU / ml. The highest total number of fungal colonies was found in rumen contents with the addition of palm oil fronds, with an average colony number of 655.83 x104 CFU / ml, while the lowest was found in buffalo rumen contents with the addition of palm fronds and palm leaves (106.67x104 CFU / ml). The addition of oil palm fronds and palm leaves to the rumen contents did not have a different effect (p> 0.05).

Komponen Serat Sabut Kelapa yang Difermentasi Menggunakan Mikroba Pencerna Serat dari Rumen Kerbau

ABSTRAK. Penelitian ini bertujuan untuk mengkaji pengaruh perbedaan lama pemeraman dan aras starter mikroba pencerna serat dari rumen kerbau terhadap komponen serat dan perubahan struktur jaringan sabut kelapa fermentasi. Percobaan menggunakan rancangan acak lengkap pola faktorial 3x3 dan 4 ulangan. Fermentasi sabut kelapa menggunakan mikroba pencerna serat dari rumen kerbau sebagai starter dengan perlakuan aras starter (0, 2,5 dan 5%) dan lama peram (0, 7 dan 14 hari). Parameter yang diamati adalah komponen serat dan perubahan struktur jaringan. Data dianalisis menggunakan analisis ragam, dilanjutkan uji jarak berganda Duncan. Hasil penelitian menunjukkan tidak terdapat pengaruh interaksi (P0,05) antara perbedaan aras starter dan lama peram terhadap kadar neutral detergent fiber (NDF), acid detergent fiber (ADF), hemiselulosa, selulosa dan lignin. Kesimpulan penelitian ini adalah penurunan kadar komponen serat serta peningkatan kerusakan jaringan seiring dengan peningkatan aras starter dan lama pemeraman. Hal ini disebabkan oleh penurunan kadar selulosa dan lignin sedangkan kadar hemiselulosa tidak dipengaruhi. Hal ini didukung oleh pengamatan terhadap perubahan struktur jaringan. (Fiber components of fermented coconut husk using fibre degrading microbes from buffalo rumen) ABSTRACT. This study aimed to examine the effect of different fermentation periods and levels of fiber-degrading microbial starter from buffalo rumen fluid on fiber component and tissue structure alteration of coconut husk. The experimental design used was factorial randomized complete 3x3 and 4 replications. The coconut husk fermentation was using fiber-degrading microbial obtained from buffalo rumen fluid as a starter with different treatments of starter levels (0, 2.5 and 5%) and fermentation periods (0, 7 and 14 days). Parameters observed were fiber component and tissue structure alteration. The data were analyzed by analysis of variance and continued by Duncan’s Multiple Range Test. The results of this research showed that there was no interaction effect (P0.05) between starter level and fermentation period on NDF, ADF, hemicellulose, cellulose and lignin contents. The conclusion of this research was decreased fiber component content and damage to tissue structure of coconut husk along with increased starter level and fermentation period. This is caused by decreased levels of cellulose and lignin while hemicellulose levels were not affected. This is supported by observations of changes in tissue structure alteration.