Symbiotic performance of native cowpea rhizobia of Ethiopia under greenhouse conditions

Green Farming ◽  
2020 ◽  
Vol 11 (2-3) ◽  
pp. 145
Author(s):  
GIRMAYE KENASA ◽  
FASSIL ASSEFA ◽  
B.C. NANDESHWAR
Keyword(s):  
Cowpea Rhizobia

scholarly journals Host-dependent Expression of Uptake Hydrogenase in Cowpea Rhizobia

Microbiology ◽  
1985 ◽  
Vol 131 (1) ◽  
pp. 93-96 ◽  
Author(s):  
F. C. GARG ◽  
R. P. GARG ◽  
K. KUKREJA ◽  
S. S. SINDHU ◽  
P. TAURO
Keyword(s):  
Uptake Hydrogenase ◽  
Cowpea Rhizobia

The transport and metabolism of glucose in cowpea rhizobia

1983 ◽  
Vol 29 (4) ◽  
pp. 398-406 ◽  
Author(s):  
Mark D. Stowers ◽  
Gerald H. Elkan
Keyword(s):  
Potassium Cyanide ◽  
Tca Cycle ◽  
Pentose Phosphate ◽  
Free Living ◽  
Phosphogluconate Dehydrogenase ◽  
Metabolism Enzymes ◽  
Carbonyl Cyanide ◽  
Cowpea Rhizobia ◽  
Enzymes Of Carbohydrate Metabolism ◽  
Free Living Conditions

Cell-free extracts of several strains of cowpea rhizobia grown under free-living conditions were surveyed for key enzymes of carbohydrate metabolism. Enzymes of the Entner–Doudoroff (ED) and Embden–Meyerhof–Parnas (EMP) pathways were detected while 6-phosphogluconate dehydrogenase was not detected, indicating the apparent absence of the pentose phosphate (PP) pathway. Transketolase and transaldolase reactions were present, indicating a pathway for the synthesis of pyrimidines and purines from fructose-6-phosphate and glyceraldehyde-3-phosphate. Radiorespirometric analysis with specifically labelled glucose indicated that the ED pathway with the hexose cycle was the primary pathway of glucose dissimilation. The presence of isocitrate and malate dehydrogenases and results of radiorespirometric analysis with pyruvate and succinate demonstrates an operational tricarboxylic acid (TCA) cycle in glucose-grown cowpea rhizobia. The transport of glucose was inhibited by carbonyl cyanide m-chlorphenylhydrazone (CCCP), dinitrophenol, and potassium cyanide indicating that the process was active, probably using an energized membrane state. The transport of glucose was glucose specific. A lower rate of glucose uptake was seen when cells were cultured on hexoses other than glucose.

scholarly journals Kinetics of Denitrifying Growth by Fast-Growing Cowpea Rhizobia

1985 ◽  
Vol 49 (3) ◽  
pp. 517-521 ◽  
Author(s):  
G. A. El Hassan ◽  
R. M. Zablotowicz ◽  
D. D. Focht
Keyword(s):  
Fast Growing ◽  
Kinetics Of ◽  
Cowpea Rhizobia

scholarly journals Phenotypic and genotypic characteristics of cowpea rhizobia from soils of Ethiopia

2018 ◽  
Vol 17 (42) ◽  
pp. 1299-1312
Author(s):  
Kenasa Girmaye ◽  
Assefa Fassil ◽  
Y. Habteselassie Mussie
Keyword(s):  
Genotypic Characteristics ◽  
Cowpea Rhizobia

Temperature and desiccation tolerance of cowpea rhizobia

1984 ◽  
Vol 30 (6) ◽  
pp. 820-823 ◽  
Author(s):  
Peter G. Hartel ◽  
Martin Alexander
Keyword(s):  
High Temperature ◽  
Vigna Unguiculata ◽  
Sandy Soil ◽  
Desiccation Tolerance ◽  
Geographical Origin ◽  
Moist Soil ◽  
Cell Numbers ◽  
Dry Areas ◽  
Cowpea Rhizobia

Strains of Rhizobium capable of nodulating cowpeas (Vigna unguiculata) were tested for their ability to tolerate desiccation and high temperature in soil. Eight strains were from a hot, dry region of Niger, and 13 strains were from cooler, wetter regions of Nigeria. All of the 21 strains tested at 30 °C grew somewhat in a moist sandy soil from Niger. Of the 21 strains tested in moist soil incubated at 37 °C, only the 8 strains from Niger did not decline in numbers. The moist soil was then allowed to dry slowly. After a 2-week period, the decline in the logarithm of cell numbers per gram of soil ranged from 0.19 to 0.41 at 30 °C and from 0.28 to 0.66 at 37 °C for the 8 strains from Niger and from 0.48 to 2.42 at 30 °C and 0.87 to 2.85 at 37 °C for the 13 strains from Nigeria. These results suggest that cowpea rhizobia from hot, dry areas are more temperature and desiccation tolerant than strains from cooler, more humid regions and that temperature and desiccation tolerance may be related to geographical origin.

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