Evaluation of Serum B12, Folic Acid, Iron, Ferritin, Total Iron Binding Capacity and Unsaturated Iron Binding Capacity in Patients with Recurrent Aphthous Stomatitis in Sulaimani City

Abstract Radiometric, colorimetric, and two immunochemical methods for measuring total iron-binding capacity are compared. We evaluated the procedures on the basis of precision, applicability to a pediatric population, and accuracy as assessed by analytical recovery of purified transferrin. The immunoephelometric assay for transferrin provides significant advantages over the other methods examined.

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Ferrozine iron and total iron-binding capacity method adapted to the ABA-100 Bichromatic Analyzer.

Clinical Chemistry ◽

10.1093/clinchem/27.8.1441 ◽

1981 ◽

Vol 27 (8) ◽

pp. 1441-1444 ◽

Cited By ~ 4

Author(s):

L Mori ◽

A Bekkering ◽

J Traini ◽

L Vanderlinden

Keyword(s):

Binding Capacity ◽

Magnesium Carbonate ◽

Total Iron ◽

Iron Binding ◽

Total Iron Binding Capacity ◽

Standard Curve ◽

Excess Iron ◽

Color Reagent ◽

Iron Binding Capacity ◽

Capacity Method

Abstract A sensitive method (Clin. Chem. 26: 327--331, 1980) for serum iron, in which the color reagent Ferrozine is used, is modified and adapted to the Abbott ABA-100 discrete analyzer. The standard curve is linear to at least 10 mg/L and the method showed day-to-day precision (CV) of 2.4% for a 1.03 mg/L sample (n = 63) and 1.9% for a 2.13 mg/L sample (n = 63). Lower values were obtained than with the modified continuous-flow technique of Giovanniello et al., but the correlation was good (r = 0.98). Bilirubin and copper do not interfere; hemoglobin and gross lipemia interfere only slightly. The total iron-binding capacity, based on Ramsay's method, was evaluated with regard to the effect of adding various amounts of magnesium carbonate. Results led us to use a ratio of approximately 180 mg of magnesium carbonate to each 5 micrograms of excess iron added. Day-to-day, the method for total iron-binding capacity gave a CV of 3.1% for a 2.55 mg/L sample, 2.8% for a 3.63 mg/L sample.

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Serum iron and total iron-binding capacity compared with serum ferritin in assessment of iron deficiency.

Clinical Chemistry ◽

10.1093/clinchem/27.2.276 ◽

1981 ◽

Vol 27 (2) ◽

pp. 276-279 ◽

Cited By ~ 15

Author(s):

F Peter ◽

S Wang

Keyword(s):

Iron Deficiency ◽

Serum Ferritin ◽

Serum Iron ◽

Binding Capacity ◽

Transferrin Saturation ◽

Total Iron ◽

Iron Binding ◽

Total Iron Binding Capacity ◽

Iron Deficient ◽

Iron Binding Capacity

Abstract Ferritin values for 250 selected sera were compared with values for iron, total iron-binding capacity (TIBC), and transferrin saturation, to assess the potential of the ferritin assay for the detection of latent iron deficiency. The specimens were grouped (50 in each group) according to their values for iron and TIBC. In Group 1 (low iron, high TIBC) the saturation and ferritin values both indicated iron deficiency in all but one. In the 100 specimens of Groups 2 (normal iron, high TIBC) and 4 (normal iron, high normal TIBC), the saturation values revealed 16 iron-deficient cases, the ferritin test 55. For Groups 3 (low iron, normal TIBC) and 5 (low iron, low TIBC), the ferritin test revealed fewer cases of iron deficiency than did the saturation values (37 cases vs 51 cases, in the 100 specimens). Evidently the ferritin test detects iron deficiency in many cases for whom the serum iron and TIBC tests are not positively indicative. The correlation of serum ferritin with iron, TIBC, and transferrin saturation in the five groups was good only in the case of specimens for which the TIBC was normal; if it was abnormal the correlation was very poor.

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