scholarly journals Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

10.3791/52782 ◽  
2015 ◽  
Author(s):  
Guillaume Luxardi ◽  
Brian Reid ◽  
Fernando Ferreira ◽  
Pauline Maillard ◽  
Min Zhao
Keyword(s):  
Ion Fluxes ◽  
Microelectrode Technique

scholarly journals Extracellular ATP perturbs transmembrane ion fluxes, elevates cytosolic [Ca2+], and inhibits phagocytosis in mouse macrophages.

1985 ◽  
Vol 260 (25) ◽  
pp. 13442-13449 ◽  
Author(s):  
S S Sung ◽  
J D Young ◽  
A M Origlio ◽  
J M Heiple ◽  
H R Kaback ◽  
...  
Keyword(s):  
Extracellular Atp ◽  
Ion Fluxes ◽  
Mouse Macrophages

Dependence of ion fluxes on fluid transport by rat proximal tubule

1986 ◽  
Vol 250 (4) ◽  
pp. F680-F689 ◽  
Author(s):  
K. Bomsztyk ◽  
F. S. Wright
Keyword(s):  
Proximal Tubule ◽  
Fluid Transport ◽  
Rat Kidney ◽  
Water Flux ◽  
Fluid Secretion ◽  
Proximal Convoluted Tubule ◽  
Ion Fluxes ◽  
Fluid Absorption ◽  
Fluid Flux ◽  
K Transport

The effects of changes in transepithelial water flux (Jv) on sodium, chloride, calcium, and potassium transport by the proximal convoluted tubule were examined by applying a microperfusion technique to surface segments in kidneys of anesthetized rats. Perfusion solutions were prepared with ion concentrations similar to those in fluid normally present in the later parts of the proximal tubule. Osmolality of the perfusate was adjusted with mannitol. With no mannitol in the perfusates, net fluid absorption was observed. Addition of increasing amounts of mannitol first reduced Jv to zero and then reversed net fluid flux. At the maximal rates of fluid absorption, net absorption of Na, Cl, Ca, and K was observed. When Jv was reduced to zero, Na, Cl, and Ca absorption were reduced and K entered the lumen. Na, Cl, and Ca secretion occurred in association with the highest rates of net fluid secretion. The lumen-positive transepithelial potential progressively increased as the net fluid flux was reduced to zero and then reversed. The results demonstrate that changes in net water flux can affect Na, Cl, Ca, and K transport by the proximal convoluted tubule of the rat kidney. These changes in net ion fluxes are not entirely accounted for by changes in bulk-phase transepithelial electrochemical gradients.

Voltamperommetric study of chemically made polyaniline powder with cavity microelectrode technique

2002 ◽  
Vol 126 (2-3) ◽  
pp. 253-262 ◽  
Author(s):  
V. Vivier ◽  
C. Cachet-Vivier ◽  
D. Michel ◽  
J.-Y. Nedelec ◽  
L.T. Yu
Keyword(s):  
Microelectrode Technique ◽  
Cavity Microelectrode

scholarly journals Effect of cimetidine on ion fluxes and potential difference across the human stomach

Gut ◽  
1978 ◽  
Vol 19 (5) ◽  
pp. 414-418 ◽  
Author(s):  
K. J. Ivey ◽  
P. A. Mackercher
Keyword(s):  
Human Stomach ◽  
Potential Difference ◽  
Ion Fluxes

scholarly journals Quantitative Observation of Electric Potential Distribution of Brittle Polyelectrolyte Hydrogels Using Microelectrode Technique

2016 ◽  
Vol 49 (8) ◽  
pp. 3100-3108 ◽  
Author(s):  
Honglei Guo ◽  
Takayuki Kurokawa ◽  
Masakazu Takahata ◽  
Wei Hong ◽  
Yoshinori Katsuyama ◽  
...  
Keyword(s):  
Electric Potential ◽  
Potential Distribution ◽  
Electric Potential Distribution ◽  
Microelectrode Technique

scholarly journals Time-averaged and time-resolved ion fluxes related to reactive HiPIMS deposition of Ti-Al-N films

2021 ◽  
pp. 127638
Author(s):  
L. Zauner ◽  
A. Bahr ◽  
T. Kozák ◽  
J. Čapek ◽  
T. Wojcik ◽  
...  
Keyword(s):  
Ion Fluxes ◽  
Time Resolved

Stomatal movements and ion fluxes within epidermis of Commelina communis L.

Nature ◽  
1974 ◽  
Vol 252 (5479) ◽  
pp. 126-127 ◽  
Author(s):  
C. M. WILLMER ◽  
J. E. PALLAS
Keyword(s):  
Ion Fluxes ◽  
Commelina Communis ◽  
Stomatal Movements

Effect of pyrithiamine treatment of potassium ion fluxes in rat cortical slices

1984 ◽  
Vol 773 (1) ◽  
pp. 125-131 ◽  
Author(s):  
James M. Gale ◽  
Ronald W. Brosemer
Keyword(s):  
Potassium Ion ◽  
Ion Fluxes ◽  
Rat Cortical Slices ◽  
Cortical Slices

Ionic control of sperm function

1995 ◽  
Vol 7 (4) ◽  
pp. 905 ◽  
Author(s):  
LR Fraser
Keyword(s):  
Ionic Composition ◽  
Ion Fluxes ◽  
Sperm Function ◽  
Considerable Evidence ◽  
Functional Potential ◽  
Mammalian Sperm ◽  
Acrosomal Exocytosis ◽  
Extracellular Environment

Successful sperm function leads to fertilization. It is dependent on the extracellular environment, especially the array and concentration of various ions. Considerable evidence indicates that this is because of consequent effects on the intracellular ionic composition. Although both cations and anions undoubtedly play a role in a modulating sperm function, most of the evidence currently available concerns cations. Therefore, this review will concentrate on cations, focussing on Ca2+, Na+, K+ and H+. Their requirements for successful capacitation (mammalian sperm) and acrosomal exocytosis (both invertebrate and mammalian sperm) will be considered. In particular, the mechanisms which may control ion fluxes, leading to changes in the intracellular ionic composition and subsequently to changes in sperm functional potential, will be addressed.

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