Investigating Teliospore Germination Using Microrespiration Analysis and Microdissection

Puccinia horiana Henn., a quarantine-significant fungal pathogen and causal agent of chrysanthemum white rust (CWR), was first discovered in the United States in 1977 and later believed to have been eradicated. Recently, however, the disease has sporadically reappeared in the northeastern US. Possible explanations for the reappearance include survival of the pathogen in the local environment, and reintroduction from other locations. To determine the possibility that the pathogen might be overwintering in the field, we undertook the study described here. Results from the study showed that P. horiana teliospores, imbedded in infected leaves, were capable of sporulating 2 weeks after inoculation, and this capacity continued until the leaf became necrotic and desiccated. This is the first report of the extreme susceptibility of P. horiana teliospores to leaf necrosis and desiccation and suggests that field infections following winter are unlikely to originate from teliospores. Teliospore germination on excised leaves was shown to be inhibited by light. Accepted for publication 3 April 2013. Published 23 August 2013.

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The Cintractia pattern of teliospore germination

Mycological Research ◽

10.1017/s0953756298008260 ◽

1999 ◽

Vol 103 (8) ◽

pp. 1071-1072 ◽

Cited By ~ 1

Author(s):

C.T. Ingold

Keyword(s):

Teliospore Germination

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Teliospore Germination, Cytology and Development of Entyloma eleocharidis

CYTOLOGIA ◽

10.1508/cytologia.35.391 ◽

1970 ◽

Vol 35 (3) ◽

pp. 391-401 ◽

Cited By ~ 4

Author(s):

M. S. Pavgi ◽

R. A. Singh

Keyword(s):

Teliospore Germination

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Enhancement of Teliospore Germination in Wheat- and Wild Grass-Infecting Species of Tilletia on Activated Charcoal Medium

Phytopathology ◽

10.1094/phyto.1998.88.3.260 ◽

1998 ◽

Vol 88 (3) ◽

pp. 260-264 ◽

Cited By ~ 5

Author(s):

María L. Boyd ◽

Lori M. Carris

Keyword(s):

Bovine Serum Albumin ◽

Serum Albumin ◽

Activated Charcoal ◽

Bovine Serum ◽

Germination Percentage ◽

Polyvinyl Pyrrolidone ◽

Wild Grass ◽

Teliospore Germination ◽

Lag Period

The effect of activated charcoal as an amendment to water agar medium on teliospore germination was analyzed for two species of wheat-infecting bunts, Tilletia controversa and T. tritici, and two related wild-grass infecting species, T. bromi and T. fusca. Final percentages of teliospore germination, area under the germination progress curves (AUGPC), and a standardized AUGPC (SAUGPC) on carbon agar and water agar were compared among strains. Carbon agar (CA) significantly increased the final germination percentage of teliospores, AUGPC, and SAUGPC when compared with water agar (WA) for all taxa under study. Additionally, CA reduced significantly the incubation (i.e., lag) period when compared with WA for teliospores of T. bromi, T. controversa, and T. fusca. Bovine serum albumin and polyvinyl pyrrolidone were used as alternative chemical adsorbent amendments to WA to establish the role of activated charcoal in the medium. Only media amended with bovine serum albumin and activated charcoal improved the final germination percentage of all taxa. Polyvinyl pyrrolidone was not significantly better than water agar.

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