In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

10.3791/60551 ◽  
2019 ◽  
Author(s):  
Daniela Avila-González ◽  
Guadalupe García-López ◽  
Néstor E. Díaz-Martínez ◽  
Héctor Flores-Herrera ◽  
Anayansi Molina-Hernández ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
In Vitro Culture ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane

In Vitro Antiangiogenic Activity in Ex Vivo Expanded Human Limbocorneal Epithelial Cells Cultivated on Human Amniotic Membrane

2004 ◽  
Vol 45 (8) ◽  
pp. 2586 ◽  
Author(s):  
David Hui-Kang Ma ◽  
Jeng-Yuan Yao ◽  
Long-Kuen Yeh ◽  
Sung-Tzu Liang ◽  
Lai-Chu See ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Amniotic Membrane ◽  
Ex Vivo ◽  
Human Amniotic Membrane ◽  
Antiangiogenic Activity

scholarly journals Human Amniotic Membrane Extracts have Anti-Inflammatory Effects on Damaged Human Corneal Epithelial Cells In Vitro

2016 ◽  
Vol 25 (3) ◽  
pp. 282-287 ◽  
Author(s):  
Han-Na Lee ◽  
Raymundo Bernardo ◽  
Gi-Yeon Han ◽  
Gyu-Young Kim ◽  
Ji-Soo Kim ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells ◽  
Anti Inflammatory

scholarly journals A Novel Phenotype of Junctional Epidermolysis Bullosa with Transient Skin Fragility and Predominant Ocular Involvement Responsive to Human Amniotic Membrane Eyedrops

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 716
Author(s):  
Daniele Castiglia ◽  
Paola Fortugno ◽  
Angelo Giuseppe Condorelli ◽  
Sabina Barresi ◽  
Naomi De Luca ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Epidermolysis Bullosa ◽  
Ocular Involvement ◽  
Human Amniotic Membrane ◽  
Vitro Assay ◽  
Junctional Epidermolysis Bullosa ◽  
Mucosal Involvement ◽  
Skin Fragility ◽  
Laminin 332

Junctional epidermolysis bullosa (JEB) is a clinically and genetically heterogeneous skin fragility disorder frequently caused by mutations in genes encoding the epithelial laminin isoform, laminin-332. JEB patients also present mucosal involvement, including painful corneal lesions. Recurrent corneal abrasions may lead to corneal opacities and visual impairment. Current treatments are merely supportive. We report a novel JEB phenotype distinguished by the complete resolution of skin fragility in infancy and persistent ocular involvement with unremitting and painful corneal abrasions. Biallelic LAMB3 mutations c.3052-5C>G and c.3492_3493delCG were identified as the molecular basis for this phenotype, with one mutation being a hypomorphic splice variant that allows residual wild-type laminin-332 production. The reduced laminin-332 level was associated with impaired keratinocyte adhesion. Then, we also investigated the therapeutic power of a human amniotic membrane (AM) eyedrop preparation for corneal lesions. AM were isolated from placenta donors, according to a procedure preserving the AM biological characteristics as a tissue, and confirmed to contain laminin-332. We found that AM eyedrop preparation could restore keratinocyte adhesion in an in vitro assay. Of note, AM eyedrop administration to the patient resulted in long-lasting remission of her ocular manifestations. Our findings suggest that AM eyedrops could represent an effective, non-invasive, simple-to-handle treatment for corneal lesions in patients with JEB and possibly other EB forms.

In vitro culture and expansion of human limbal epithelial cells

2010 ◽  
Vol 5 (8) ◽  
pp. 1470-1479 ◽  
Author(s):  
Indumathi Mariappan ◽  
Savitri Maddileti ◽  
Soumya Savy ◽  
Shubha Tiwari ◽  
Subhash Gaddipati ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
In Vitro Culture

Embryo co-culture with bovine amniotic membrane stem cells can enhance the cryo-survival of IVF-derived bovine blastocysts comparable with co-culture with bovine oviduct epithelial cells

Zygote ◽  
2020 ◽  
pp. 1-6
Author(s):  
Shayan Nejat-Dehkordi ◽  
Ebrahim Ahmadi ◽  
Abolfazl Shirazi ◽  
Hassan Nazari ◽  
Naser Shams-Esfandabadi
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Epithelial Cells ◽  
Embryonic Development ◽  
Amniotic Membrane ◽  
Biological Activities ◽  
Survival Rates ◽  
Blastocyst Stage ◽  
Oviduct Epithelial Cells

Summary Culture conditions have a profound effect on the quality of in vitro-produced embryos. Co-culturing embryos with somatic cells has some beneficial effects on embryonic development. Considering the ability of stem cells to secrete a broad range of growth factors with different biological activities, we hypothesized that bovine amniotic membrane stem cells (bAMSCs) might be superior to bovine oviduct epithelial cells (BOECs) in supporting embryonic development and enhancing their cryo-survival. Bovine abattoir-derived oocytes were matured and fertilized in vitro. The resultant presumptive zygotes were then cultured up to the blastocyst stage in the following groups: (i) co-culture with bAMSCs, (ii) co-culture with BOECs, and (iii) cell-free culture (Con). Embryos that reached the blastocyst stage were vitrified and warmed, and their post-warming re-expansion, survival and hatching rates were evaluated after 72 h culture. Results showed that the cleavage, blastocyst, and 2 h post-warming re-expansion rates of embryos did not differ between groups. However, their survival rates in BOEC and bAMSC groups were significantly higher compared with the control (72.7, 75.6 and 37.5%, respectively, P < 0.05). In conclusion, our results showed that the cryo-survivability of IVF-derived bovine embryos could be improved through co-culturing with bAMSCs. Moreover, considering the possibility to provide multiple passages from bAMSCs compared with BOECs, due to their stemness properties and their ability to produce growth factors, the use of bAMSCs is a good alternative to BOECs in embryo co-culture systems.

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