Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

10.3791/60663 ◽  
2020 ◽  
Author(s):  
Fengwei Zhang ◽  
Yanan Wang ◽  
Xuechai Shang ◽  
Shanshan Wang ◽  
Rong Xiao ◽  
...  
Keyword(s):  
High Titer ◽  
Envelope Glycoproteins ◽  
Highly Pathogenic ◽  
Pathogenic H5n1

Comparative Study of Highly Pathogenic Avian Infl uenza Strains Isolated in Ukraine in 2005 and 2008

2014 ◽  
Vol 1 (1) ◽  
pp. 68-71
Author(s):  
A. Gerilovych ◽  
B. Stegniy ◽  
A. Stegniy ◽  
M. Stegniy ◽  
K. Smietanka ◽  
...  
Keyword(s):  
Western Europe ◽  
Rna Extraction ◽  
Experimental Studies ◽  
Molecular Characteristics ◽  
Eastern European ◽  
Highly Pathogenic ◽  
Genetic Lineages ◽  
Phylogenetic Studies ◽  
Polymerase Chain ◽  
Pathogenic H5n1

Objective. To research the molecular characteristics of two HPAI strains – A/Ch/Syvash/02/05/H5N1 and A/Ch/Krasnogvardeysk/58/08/H5N1, which were identifi ed as representatives of the highly pathogenic H5N1 viruses. Methods. RNA extraction, real-time polymerase chain reaction (PCR). Results. The phylogenetic studies revealed that the above mentioned strains belong to two various genetic lineages originated from the Eastern European strains isolated in 2005, but differ from the viruses introduced to the Central and Western Europe in 2005/2006, and also the lineages consisting of H5N1 viruses isolated in the Europe and Middle East in late 2007. Conclusions. Relying on experimental studies, it can be concluded that the strains of A/Ch/Syvash/02/05/H5N1 and A/Ch/Krasnogvardeysk/58/08/H5N1 are highly pathogenic.

Prophylactic effects of chitin microparticles on highly pathogenic H5N1 influenza virus

2007 ◽  
Vol 79 (6) ◽  
pp. 811-819 ◽  
Author(s):  
Takeshi Ichinohe ◽  
Noriyo Nagata ◽  
Peter Strong ◽  
Shin-ichi Tamura ◽  
Hidehiro Takahashi ◽  
...  
Keyword(s):  
Influenza Virus ◽  
H5n1 Influenza Virus ◽  
Highly Pathogenic ◽  
H5n1 Influenza ◽  
Pathogenic H5n1

scholarly journals Antibody responses to avian influenza viruses in wild birds broaden with age

2016 ◽  
Vol 283 (1845) ◽  
pp. 20162159 ◽  
Author(s):  
Sarah C. Hill ◽  
Ruth J. Manvell ◽  
Bodo Schulenburg ◽  
Wendy Shell ◽  
Paul S. Wikramaratna ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Haemagglutination Inhibition ◽  
Wild Birds ◽  
Influenza Viruses ◽  
Antibody Responses ◽  
Immune Recognition ◽  
Avian Influenza Viruses ◽  
Highly Pathogenic ◽  
Age Related ◽  
Pathogenic H5n1

For viruses such as avian influenza, immunity within a host population can drive the emergence of new strains by selecting for viruses with novel antigens that avoid immune recognition. The accumulation of acquired immunity with age is hypothesized to affect how influenza viruses emerge and spread in species of different lifespans. Despite its importance for understanding the behaviour of avian influenza viruses, little is known about age-related accumulation of immunity in the virus's primary reservoir, wild birds. To address this, we studied the age structure of immune responses to avian influenza virus in a wild swan population ( Cygnus olor ), before and after the population experienced an outbreak of highly pathogenic H5N1 avian influenza in 2008. We performed haemagglutination inhibition assays on sampled sera for five avian influenza strains and show that breadth of response accumulates with age. The observed age-related distribution of antibody responses to avian influenza strains may explain the age-dependent mortality observed during the highly pathogenic H5N1 outbreak. Age structures and species lifespan are probably important determinants of viral epidemiology and virulence in birds.

Highly Pathogenic (H5N1) Avian Influenza Induces an Inflammatory T Helper Type 1 Cytokine Response in the Chicken

2011 ◽  
Vol 31 (4) ◽  
pp. 393-400 ◽  
Author(s):  
Adam J. Karpala ◽  
John Bingham ◽  
Karel A. Schat ◽  
Li-Mei Chen ◽  
Ruben O. Donis ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Cytokine Response ◽  
T Helper ◽  
Highly Pathogenic ◽  
H5n1 Avian Influenza ◽  
Type 1 Cytokine ◽  
Pathogenic H5n1 ◽  
Helper Type

scholarly journals Baculoviral Co-Expression of HA, NA and M1 Proteins of Highly Pathogenic H5N1 Influenza Virus in Insect Cells

2013 ◽  
Vol 6 (9) ◽  
Author(s):  
Farida Behzadian ◽  
Zahra Goodarzi ◽  
Fatemeh Fotouhi ◽  
Esmaeil Saberfar
Keyword(s):  
Influenza Virus ◽  
Insect Cells ◽  
H5n1 Influenza Virus ◽  
Highly Pathogenic ◽  
H5n1 Influenza ◽  
Pathogenic H5n1

An outbreak of highly pathogenic H5N1 avian influenza in Korea, 2008

2010 ◽  
Vol 141 (3-4) ◽  
pp. 362-366 ◽  
Author(s):  
Hye-Ryoung Kim ◽  
Choi-Kyu Park ◽  
Youn-Jeong Lee ◽  
Gye-Hyeong Woo ◽  
Kyoung-Ki Lee ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Highly Pathogenic ◽  
H5n1 Avian Influenza ◽  
Pathogenic H5n1
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