A Modified Surgical Model of Hind Limb Ischemia in ApoE-/- Mice using a Miniature Incision

10.3791/62402 ◽  
2021 ◽  
Author(s):  
Kaixuan Yan ◽  
Jiaxing Zheng ◽  
Frank G. Zöllner ◽  
Kay Schwenke ◽  
Prama Pallavi ◽  
...  
Keyword(s):  
Hind Limb ◽  
Limb Ischemia ◽  
Hind Limb Ischemia ◽  
Surgical Model

scholarly journals FACS-purified myoblasts producing controlled VEGF levels induce safe and stable angiogenesis in chronic hind limb ischemia

2011 ◽  
Vol 16 (1) ◽  
pp. 107-117 ◽  
Author(s):  
Thomas Wolff ◽  
Edin Mujagic ◽  
Roberto Gianni-Barrera ◽  
Philipp Fueglistaler ◽  
Uta Helmrich ◽  
...  
Keyword(s):  
Hind Limb ◽  
Limb Ischemia ◽  
Hind Limb Ischemia

scholarly journals WITHDRAWN: Melatonin attenuates lung injury in a hind limb ischemia–reperfusion rat model

2014 ◽  
Author(s):  
Hamed Takhtfooladi ◽  
Mohammad Takhtfooladi ◽  
Fariborz Moayer ◽  
Sayed Mobarakeh
Keyword(s):  
Lung Injury ◽  
Rat Model ◽  
Hind Limb ◽  
Ischemia Reperfusion ◽  
Limb Ischemia ◽  
Hind Limb Ischemia

Abstract 17597: Endogenous Del-1 is a Negative Regulator of Ischemia-induced Angiogenesis by Interacting With the Leukocytic LFA-1 Integrin

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Sebastian Cremer ◽  
Anne Klotzsche-von Ameln ◽  
Alessia Orlandi ◽  
Irina Korovina ◽  
Bettina Gercken ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Hind Limb ◽  
Hematopoietic Cells ◽  
Limb Ischemia ◽  
Inflammatory Cells ◽  
Capillary Density ◽  
Negative Regulator ◽  
Hind Limb Ischemia ◽  
Autonomous Pathway

Developmental endothelial locus-1 (Del-1) is an endothelial cell-derived secreted protein circulating in blood and associated with the cell surface and the extracellular matrix. As we previously demonstrated, Del-1 restricts leukocyte recruitment by inhibiting the β2-integrin, LFA-1. Leukocytes and progenitor cells (PC) may contribute to angiogenesis. The role of endogenous Del-1 in angiogenesis is elusive. We found, that physiological angiogenesis of the developing retina was not affected in the Del-1-/- mice compared to the wildtype (WT) mice. Surprisingly, Del-1-/- mice displayed a significantly increased angiogenic response compared to WT mice after induction of hind limb ischemia (144 ± 6 % increase of capillary density) and retinal ischemia (retinopathy of prematurity model) suggesting that endogenous Del-1 is an inhibitor of ischemia-induced neovascularization. Silencing of Del-1 with siRNA did not affect the angiogenic sprouting of endothelial cell (EC) spheroids, indicating that Del-1 blocks angiogenesis in a non-endothelial cell autonomous pathway. Soluble Del-1 blocked the adhesion of inflammatory cells on EC monolayers. In line with these results, ischemic muscles and ischemic retinae from Del-1-/- mice displayed an enhanced infiltration with inflammatory cells compared to the WT mice. Since Del-1 blocks inflammatory cell homing by inhibiting the leukocytic LFA-1-integrin, we addressed the role of the Del-1/LFA-1-integrin interaction on the inhibitory function of endogenous Del-1 on angiogenesis. Indeed, Del-1/LFA-1-double deficiency reversed the pro-angiogenic phenotype of the Del-1-/- mice to the level of WT mice in the model of hind limb ischemia. Thus, the inhibitory role of Del-1 on neovascularization is mediated by the interaction of Del-1 with the LFA-1-integrin. Moreover, Del-1-deficiency led to an increased homing of intravenously injected murine fluorescence-labeled WT Lin- BM PC in ischemic muscles in comparison to WT mice after the induction of hind limb ischemia. Taken together, Del-1 acts as a negative regulator of ischemia-induced angiogenesis by interacting with the LFA-1-integrin expressed in hematopoietic cells, thereby inhibiting the homing of hematopoietic cells to ischemic tissues.

scholarly journals Apolipoprotein E–/– mice have delayed skeletal muscle healing after hind limb ischemia–reperfusion

2008 ◽  
Vol 48 (3) ◽  
pp. 701-708 ◽  
Author(s):  
Jeanwan Kang ◽  
Hassan Albadawi ◽  
Virendra I. Patel ◽  
Thomas A. Abbruzzese ◽  
Jin-Hyung Yoo ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Apolipoprotein E ◽  
Hind Limb ◽  
Ischemia Reperfusion ◽  
Limb Ischemia ◽  
Hind Limb Ischemia

scholarly journals Hydrogen Sulfide Reduces Neutrophil Recruitment in Hind-Limb Ischemia-Reperfusion Injury in an L-Selectin and ADAM-17–Dependent Manner

2013 ◽  
Vol 131 (3) ◽  
pp. 487-497 ◽  
Author(s):  
Carissa J. Ball ◽  
Alyssa J. Reiffel ◽  
Sathvika Chintalapani ◽  
Minsoo Kim ◽  
Jason A. Spector ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Reperfusion Injury ◽  
Hind Limb ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Limb Ischemia ◽  
Neutrophil Recruitment ◽  
Dependent Manner ◽  
Hind Limb Ischemia

scholarly journals The Effect of p27kip1 on Arterial Remodeling in Response to Hind Limb Ischemia

2014 ◽  
Vol 60 (2) ◽  
pp. 537
Author(s):  
Galit Ankri-Eliahoo ◽  
Kevin Weitz ◽  
Gale Tang
Keyword(s):  
Hind Limb ◽  
Limb Ischemia ◽  
Arterial Remodeling ◽  
Hind Limb Ischemia

scholarly journals Proangiogenic role of PPARá in the mouse hind limb ischemia model

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Yasmeen Rizvi ◽  
Adebayo Oyekan
Keyword(s):  
Hind Limb ◽  
Limb Ischemia ◽  
Hind Limb Ischemia
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