Automated real-time PCR to determine K-ras codon 12 mutations in non-small cell lung cancer: Comparison with immunohistochemistry and clinico-pathological features

2003 ◽  
Author(s):  
Wolfgang Hilbe ◽  
Margit Dlaska ◽  
Hans-Christoph Duba ◽  
Stephan Dirnhofer ◽  
Wolfgang Eisterer ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Real Time ◽  
Cell Lung Cancer ◽  
Real Time Pcr ◽  
Small Cell ◽  
Pathological Features ◽  
Small Cell Lung

scholarly journals Determination of EGFR gene somatic mutations in tissues and plasma of patients with advanced non-small cell lung cancer

2016 ◽  
Vol 62 (6) ◽  
pp. 638-644
Author(s):  
O.I. Brovkina ◽  
M.G. Gordiev ◽  
A.N. Toropovskiy ◽  
D.S. Khodyrev ◽  
R.F. Enikeev ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Real Time ◽  
Cell Lung Cancer ◽  
Real Time Pcr ◽  
Test System ◽  
Small Cell ◽  
Egfr Mutations ◽  
Small Cell Lung ◽  
Egfr Gene

The presence of activating mutations in the EGFR gene influences cell proliferation, angiogenesis, and increases metastatic ability; it has a significant impact on the choice of medical therapy of non-small cell lung cancer (NSCLC). The use of targeted therapy with tyrosine kinase inhibitors requires performance of appropriate genetic tests. The aim of this study was to design a real-time PCR-based diagnostic kit for fast and cheap of EGFR mutations testing in paraffin blocks and plasma, and kit validation using samples from patients with NSCLC, and also comparative estimation of diagnostic features of real-time PCR with wild type blocking and digital PCR for mutation testing in blood plasma. The study included 156 patients with various types of adenocarcinoma differentiation. It was designed a simple and efficient real-time PCR-based method of detecting L858R activating mutation and del19 deletion in the EGFR gene for DNA isolated from paraffin blocks. Kit for EGFR mutations was validated using 411 samples of paraffin blocks. The proposed system showed high efficiency for DNA testing from paraffin blocks: a concordance with results of testing with therascreen® EGFR RGQ PCR Kit (`Qiagen`, Germany) was 100%. It has been shown the possibility of using this test system for the detection of mutations in plasma

66PD PRELIMINARY REAL-TIME PCR EVALUATION OF FREE-CIRCULATING PLASMA DNA IN HEALTHY INDIVIDUALS AND PATIENTS WITH RESECTABLE NON-SMALL CELL LUNG CANCER

Lung Cancer ◽  
2009 ◽  
Vol 64 ◽  
pp. S33
Author(s):  
A. Szpechcinski ◽  
M. Dancewicz ◽  
P. Jagus ◽  
E. Polgesek ◽  
P. Kopinski ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Real Time ◽  
Cell Lung Cancer ◽  
Real Time Pcr ◽  
Small Cell ◽  
Healthy Individuals ◽  
Plasma Dna ◽  
Small Cell Lung

Comparative Quantification of Plasma hnRNP B1 mRNA in Non-small Cell Lung Cancer Patients by Real-time PCR

2009 ◽  
Vol 29 (3) ◽  
pp. 249-255 ◽  
Author(s):  
Jeong-Man Kim ◽  
Sang Hyun Hwang ◽  
Eun Ju Song ◽  
Lee Sang-Yull ◽  
Yeong-Dae Kim ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Real Time ◽  
Cancer Patients ◽  
Cell Lung Cancer ◽  
Real Time Pcr ◽  
Small Cell ◽  
Small Cell Lung ◽  
Lung Cancer Patients

scholarly journals Prognostic Impact of Melatonin Receptors MT1 and MT2 in Non-Small Cell Lung Cancer (NSCLC)

Cancers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1001 ◽  
Author(s):  
Jablonska ◽  
Nowinska ◽  
Piotrowska ◽  
Partynska ◽  
Katnik ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Real Time ◽  
Cell Lung Cancer ◽  
Real Time Pcr ◽  
Small Cell ◽  
Melatonin Receptors ◽  
Prognostic Impact ◽  
Cancer Stage ◽  
Small Cell Lung

Background: Several studies have investigated the inhibitory effect of melatonin on lung cancer cells. There are no data available on the prognostic impact of melatonin receptors MT1 and MT2 in non-small cell lung cancer (NSCLC). Materials and Methods: Immunohistochemical studies of MT1 and MT2 were conducted on NSCLC (N = 786) and non-malignant lung tissue (NMLT) (N = 120) using tissue microarrays. Molecular studies were performed on frozen fragments of NSCLC (N = 62; real time PCR), NMLT (N = 24) and lung cancer cell lines NCI-H1703, A549 and IMR-90 (real time PCR, western blot). Results: The expression of both receptors was higher in NSCLC than in NMLT. Higher MT1 and MT2 expression levels (at protein and mRNA) were noted in squamous cell carcinomas (SCC) compared to adenocarcinomas (AC). MT1 immunoexpression decreased as both the tumour size and the cancer stage increased in the whole cohort, while MT2 decreased as the cancer stage increased, with lymph node involvement (in the whole study group) and increasing malignancy grade (in SCC). Higher expression of MT2 was associated with a favorable prognosis. MT2 was an independent prognostic factor for overall survival (OS) in all analyzed NSCLC and in smoking patients. Conclusions: Our observations may point to the potential prognostic significance of MT2 in NSCLC.

scholarly journals B7-04: Detection of EGFR mutations in plasma by allele-specific real-time PCR in non-small cell lung cancer (NSCLC)

2007 ◽  
Vol 2 (8) ◽  
pp. S355-S356
Author(s):  
Philip C. Mack ◽  
William S. Holland ◽  
Angela M. Davies ◽  
Nichole C. Farneth ◽  
Oliver Gautschi ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Real Time ◽  
Cell Lung Cancer ◽  
Real Time Pcr ◽  
Small Cell ◽  
Egfr Mutations ◽  
Small Cell Lung ◽  
Allele Specific

Evaluation of Reference Genes for Normalization of Quantitative Real Time PCR in Non-Small Cell Lung Cancer

2006 ◽  
Vol 3 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Chiaki Endo ◽  
Zhifu Sun ◽  
Julian R. Molina ◽  
John R. Attewell ◽  
Ping Yang
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Real Time ◽  
Cell Lung Cancer ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Small Cell ◽  
Quantitative Real Time Pcr ◽  
Small Cell Lung

scholarly journals SPOCK2 Expression Correlates with Non-Small Cell Lung Cancer Proliferation, Invasion, and Tumorigenesis

2021 ◽  
Author(s):  
Yitong Xu ◽  
Lijie Liang ◽  
Chaonan Zhu ◽  
Chenglong Wang ◽  
Jun Jiang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Small Cell Lung Cancer ◽  
Real Time ◽  
Nude Mice ◽  
Cell Lung Cancer ◽  
Real Time Pcr ◽  
Small Cell ◽  
Small Cell Lung

Abstract Purpose: Lung cancer is one of the leading causes of death worldwide. Sparc (osteonectin), cwcv, and Kazal-like domains proteoglycan 2 (SPOCK2) play important roles in the development and progression of various types of human cancers. However, the role of SPOCK2 in non-small-cell lung cancer (NSCLC) is unclear. Hence, we aimed to elucidate the role of SPOCK2 in NSCLC. Methods: Real-time PCR and immunohistochemistry were used to study the relationship between SPOCK2 expression and the clinicopathology of NSCLC. SPOCK2 expression was modulated using siRNA (knockdown) or pcmv6-myc-DDK-SPOCK2 (overexpression). The invasion and migration abilities of NSCLC cells were assessed using a Transwell chamber assay, and cell proliferation was studied using the MTT and colony formation assays. Furthermore, axilla and tail vein inoculation using nude mice helped elucidate the function of SPOCK2 in vivo. The expression of related proteins was analyzed using western blotting. SPOCK2 methylation was detected using real-time PCR following the treatment of cells with decitabine. Results: The mRNA and protein levels of SPOCK2 were lower in NSCLC tissues than in adjacent normal lung tissues. SPOCK2 overexpression inhibited cell proliferation, migration, and invasion in A549, H1299 cells, and nude mice. Notch and Erk signaling pathways were inhibited by SPOCK2, whereas the Hippo pathway was activated. SPOCK2 was methylated in NSCLC cell lines. SPOCK2 expression was negatively correlated with NSCLC progression. Conclusion: Taken together, our findings indicate that SPOCK2 methylation may result in its low expression, and the levels of SPOCK2 methylation may be associated with poor prognosis in NSCLC.

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