scholarly journals Hydrogen sulfide facilities production of nitric oxide via the Akt/endothelial nitric oxide synthases signaling pathway to protect human umbilical vein endothelial cells from injury by angiotensin II

2017 ◽  
Vol 16 (5) ◽  
pp. 6255-6261 ◽  
Author(s):  
Jiasen Cui ◽  
Shunjiu Zhuang ◽  
Shaohong Qi ◽  
Li Li ◽  
Junwen Zhou ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Hydrogen Sulfide ◽  
Angiotensin Ii ◽  
Signaling Pathway ◽  
Umbilical Vein ◽  
Nitric Oxide Synthases ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells ◽  
Endothelial Nitric Oxide

Glucagon-like peptide-1 affects human umbilical vein endothelial cells in high glucose by the PI3K/Akt/eNOS signaling pathway

2017 ◽  
Vol 43 (2) ◽  
pp. 119-125
Author(s):  
Jie Wu ◽  
Pingfan Guo ◽  
Tianmin He ◽  
Fanggang Cai
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
High Glucose ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Apoptosis Rate ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1 ◽  
Umbilical Vein Endothelial Cells ◽  
Endothelial Nitric Oxide

Abstract Objectives: To investigate the effects of glucagon-like peptide-1 (GLP-1) for the apoptosis of human umbilical vein endothelial cells (HUVECs) in high glucose and the related mechanisms. Material and methods: HUVECs were cultured under different conditions for 48 h. The apoptosis rate of cells was detected by flow cytometry, the expression of p-Akt and p-eNOS was measured by Western blot, and the production of nitric oxide (NO) was detected by NO assay kit. Results: HUVECs were incubated in high glucose, the apoptosis rate of cells increased, the expression of p-Akt and p-eNOS reduced, and the production of NO decreased. After GLP-1 was added into the high glucose, the apoptosis rate of cells significantly reduced, the expression of p-Akt and p-eNOS and the production of NO obviously increased. After exendin, wortmannine and L-NAME were added into high glucose and GLP-1, respectively, exendin, wortmannine and L-NAME increased the cell apoptosis, down-regulated the expression of p-Akt and p-eNOS and dropped the production of NO, except that L-NAME made no difference on the expression of p-Akt. Conclusion: GLP-1 increased the expression of Akt and endothelial nitric oxide synthase (eNOS) in HUVECs via the up-regulation of PI3K/Akt/eNOS pathway and decreased the apoptosis rate of HUVECs in high glucose.

Sign in / Sign up

Export Citation Format

Share Document