scholarly journals Involvement of RbAp48 in erythroid differentiation of murine erythroleukemia cells induced by sodium butyrate

2014 ◽  
Vol 7 (6) ◽  
pp. 1785-1789
Author(s):  
WU-LIN QI ◽  
LING-LING CAO ◽  
JIANG-JIANG HU ◽  
JIAN-YOU XUE ◽  
TING-TING SANG ◽  
...  
Keyword(s):  
Sodium Butyrate ◽  
Erythroid Differentiation ◽  
Erythroleukemia Cells ◽  
Murine Erythroleukemia ◽  
Murine Erythroleukemia Cells

scholarly journals Stability of alpha and beta globin messenger RNA during induced differentiation of mouse erythroleukemia cells

Blood ◽  
1979 ◽  
Vol 54 (4) ◽  
pp. 933-939
Author(s):  
R Gambari ◽  
RA Rifkind ◽  
PA Marks
Keyword(s):  
Messenger Rna ◽  
Erythroid Differentiation ◽  
Beta Globin ◽  
Globin Mrna ◽  
Induced Differentiation ◽  
Hexamethylene Bisacetamide ◽  
Erythroleukemia Cells ◽  
Mrna Content ◽  
Murine Erythroleukemia ◽  
Murine Erythroleukemia Cells

Murine erythroleukemia cells (MELC) are induced to express erythroid differentiation when cultured with hexamethylene bisacetamide (HMBA). Newly synthesized alpha and beta globin mRNA are both relatively stable, half-life (t1/2) greater than 50 hr, early in the course of induced differentiation. In fully induced cells there is a decrease in stability of both newly synthesized alpha and beta globin mRNA. The decay of alpha mRNA is faster, (t 1/2, 10--12 hr) than beta globin mRNA (t1/2, 20--22 hr). Thus, differences in stability of alpha and beta globin mRNA plays a role in determining the ratio of alpha to beta mRNA content in differentiated erythroid cells.

scholarly journals Relationship between transient DNA hypomethylation and erythroid differentiation of murine erythroleukemia cells.

1988 ◽  
Vol 85 (23) ◽  
pp. 9003-9006 ◽  
Author(s):  
A. Razin ◽  
A. Levine ◽  
T. Kafri ◽  
S. Agostini ◽  
T. Gomi ◽  
...  
Keyword(s):  
Erythroid Differentiation ◽  
Dna Hypomethylation ◽  
Erythroleukemia Cells ◽  
Murine Erythroleukemia ◽  
Murine Erythroleukemia Cells

Mechanism of erythropoietin action on the erythroid progenitor cells induced from murine erythroleukemia cells (TSA8)

Development ◽  
1989 ◽  
Vol 105 (1) ◽  
pp. 109-114 ◽  
Author(s):  
H. Fukumoto ◽  
Y. Matsui ◽  
M. Obinata
Keyword(s):  
Progenitor Cells ◽  
Phosphodiesterase Inhibitor ◽  
Second Messenger ◽  
Erythroid Differentiation ◽  
Camp Level ◽  
Erythropoietin Receptor ◽  
Erythroid Progenitor ◽  
Erythroleukemia Cells ◽  
Murine Erythroleukemia ◽  
Murine Erythroleukemia Cells

Erythropoietin is a well-known erythroid differentiation and growth factor, but the mechanism of its action is not well understood. In this work, we have examined its mechanism of action on the erythropoietin-responsive murine erythroleukemia cells (TSA8). TSA8 cells become responsive to erythropoietin after induction with DMSO. Stimulatory effects on erythropoietin response are observed with the addition of compounds affecting the cAMP level such as forskolin, phosphodiesterase inhibitor and cholera toxin only in the presence of erythropoietin. cAMP analogues themselves show no stimulatory effect on TSA8 cells, nor does erythropoietin increase cAMP level in the cells. Thus, it is suggested that cAMP does not act as a direct second messenger for signal transduction through erythropoietin receptors, but as a stimulator of the erythropoietin receptor pathway and/or as a second messenger in combination with the receptor pathway. The mechanism for acquisition of responsiveness to growth and differentiation factors of progenitor cells is discussed.

scholarly journals Calcium influx in induced differentiation of murine erythroleukemia cells

Blood ◽  
1993 ◽  
Vol 81 (3) ◽  
pp. 783-792 ◽  
Author(s):  
B Gillo ◽  
YS Ma ◽  
AR Marks
Keyword(s):  
Calcium Channel ◽  
Calcium Influx ◽  
Cdna Probe ◽  
Erythroid Differentiation ◽  
Early Event ◽  
Induced Differentiation ◽  
Erythroleukemia Cells ◽  
Murine Erythroleukemia ◽  
Murine Erythroleukemia Cells

Abstract Murine erythroleukemia cells (MELC) have served as a model for examining the regulation of erythroid differentiation. However, the role of Ca2+ in the signal transduction pathways regulating differentiation remains unclear. To begin to address this uncertainty we have characterized the regulation of cytoplasmic Ca2+ and the possible role of calcium channels during induced differentiation in MELC. MELC can be induced to terminal differentiation using the polar/apolar compound hexamethylene bisacetamide (HMBA). We found that HMBA stimulated Ca2+ influx within 3 to 6 minutes and that Ca2+ entry was required but not sufficient for MELC growth and differentiation. Nifedipine (1 to 10 mumol/L), a calcium channel antagonist, blocked HMBA-induced Ca2+ influx and inhibited differentiation by approximately 60%. Depolarization of the MELC membrane did not induce Ca2+ influx and whole-cell patch-clamp recordings failed to detect a voltage-activated Ca2+ current, suggesting that MELC do not express detectable levels of a functional voltage-dependent calcium channel (VDCC). However, a cDNA probe encoding a portion of the alpha 1 subunit of the cardiac VDCC detected an approximately 8-kb mRNA on Northern blots of total MELC RNA. Taken together, these data show that Ca2+ influx is an early event associated with HMBA-induced differentiation in MELC, blockade of this calcium influx inhibits induced differentiation, and a voltage- insensitive dihydropyridine-sensitive calcium channel may be involved in Ca2+ influx in MELC.

Induction of erythroid differentiation in murine erythroleukemia cells by N-substituted polymethylene diamides

1979 ◽  
Vol 23 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Toyoharu Hozumi ◽  
Junko Nomura ◽  
Minoru Ishizawa
Keyword(s):  
Erythroid Differentiation ◽  
Erythroleukemia Cells ◽  
Murine Erythroleukemia ◽  
Murine Erythroleukemia Cells

Erythroid differentiation of cultured murine erythroleukemia cells by the spermine analogue canavalmine

1985 ◽  
Vol 846 (1) ◽  
pp. 101-108
Author(s):  
Shinsuke Fujihara ◽  
Toshikatsu Nakashima ◽  
Yutaka Kurogochi
Keyword(s):  
Erythroid Differentiation ◽  
Erythroleukemia Cells ◽  
Murine Erythroleukemia ◽  
Murine Erythroleukemia Cells

scholarly journals Characterization of cellular receptors for erythroid differentiation factor on murine erythroleukemia cells

1989 ◽  
Vol 264 (17) ◽  
pp. 10309-10314
Author(s):  
M Hino ◽  
A Tojo ◽  
K Miyazono ◽  
Y Miura ◽  
S Chiba ◽  
...  
Keyword(s):  
Erythroid Differentiation ◽  
Cellular Receptors ◽  
Differentiation Factor ◽  
Erythroleukemia Cells ◽  
Murine Erythroleukemia ◽  
Erythroid Differentiation Factor ◽  
Murine Erythroleukemia Cells

scholarly journals Identification and conditions for selective expression of megakaryocytic markers in Friend erythroleukemia cells

Blood ◽  
1995 ◽  
Vol 86 (7) ◽  
pp. 2624-2631 ◽  
Author(s):  
F Paoletti ◽  
AM Vannucchi ◽  
A Mocali ◽  
R Caporale ◽  
SA Burstein
Keyword(s):  
Specific Activity ◽  
Culture Media ◽  
Late Phase ◽  
Calf Serum ◽  
Erythroid Differentiation ◽  
High Serum ◽  
Hexamethylene Bisacetamide ◽  
Erythroleukemia Cells ◽  
Murine Erythroleukemia ◽  
Murine Erythroleukemia Cells

Friend murine erythroleukemia cells (MELCs) have been reevaluated in terms of their nature and potential pathways of differentiation. MELC induced with 5 mmol/L hexamethylene bisacetamide (HMBA), in addition to expression of known markers of the erythroid phenotype, were also found to exhibit traits of the megakaryocytic lineage. Erythroid differentiation was shown by the typical synthesis and accumulation of hemoglobin (Hb); megakaryoblastoid differentiation of MELCs upon induction was shown by increased specific activity of acetylcholinesterase (AChE). Incubation of MELCs with 5 mmol/L HMBA in RPMI supplemented with 1% fetal calf serum (FCS) (instead of the usual 5%), induced cells to selectively express high levels of AChE (up to approximately 170 mU/mg protein) with little activation of Hb synthesis (less than 5% B+ cells). The increase in AChE levels was a general phenomenon affecting the whole cell population and approached its maximum within 3 days of incubation with the inducer. Subsequently, MELCs become committed to terminal division, undergoing growth arrest and expression of the megakaryocytic phenotype even after the removal of HMBA. There were no appreciable changes of basal AChE levels in MELCs that were either made resistant to HMBA or treated with 0.1 mmol/L hemin that activated differentiated erythroid function without commitment. Phorbol 12-myristate 13-acetate (PMA), known to repress induced Hb synthesis in these cells, did not prevent the full increase in AChE when incubated with MELCs 2 days before HMBA addition. HMBA-induced MELCs always underwent AChE increase that was more or less pronounced depending on the low or high serum content in culture, respectively. Conversely, Hb expression was permitted only when MELCs were transferred in the late phase or at the end of commitment from low to high serum media. Variations of FCS content in culture media proved to be a simple and reliable approach to change the MELC response to inducers and to modulate expression of either megakaryocytic or mixed erythromegakaryocytic phenotype. These findings suggested that MELC might be considered, at least, as a bipotential model of differentiation to be used for studies on regulation of either megakaryocytic or erythroid markers and on competition between the two hematopoietic lineages. In this regard, it was intriguing that AChE levels attained under selective induction (low serum) were always higher than under conditions allowing coexpression of both AChE and Hb (high serum). Moreover, MELCs were also found to bind the specific rat-antimouse platelet monoclonal antibody 4A5.(ABSTRACT TRUNCATED AT 400 WORDS)

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