Quantitative assessment of HER2/neu expression by real-time PCR and fluorescent in situ hybridization analysis in low-grade osteosarcoma

2004 ◽  
Author(s):  
Woo-In Lee ◽  
Patrizia Bacchni ◽  
Franco Bertoni ◽  
Young Maeng ◽  
Yong-Koo Park
Keyword(s):  
In Situ Hybridization ◽  
Real Time ◽  
Real Time Pcr ◽  
Fluorescent In Situ Hybridization ◽  
Quantitative Assessment ◽  
Hybridization Analysis ◽  
Low Grade

scholarly journals New Real-Time Quantitative PCR Procedure for Quantification of Bifidobacteria in Human Fecal Samples

2004 ◽  
Vol 70 (7) ◽  
pp. 4165-4169 ◽  
Author(s):  
Miguel Gueimonde ◽  
Satu Tölkkö ◽  
Teemu Korpimäki ◽  
Seppo Salminen
Keyword(s):  
In Situ Hybridization ◽  
Detection Limit ◽  
Real Time ◽  
Quantitative Pcr ◽  
Real Time Pcr ◽  
Fluorescent In Situ Hybridization ◽  
The Real ◽  
Real Time Quantitative Pcr ◽  
Lanthanide Chelate

ABSTRACT The application of a real-time quantitative PCR method (5′ nuclease assay), based on the use of a probe labeled at its 5′ end with a stable, fluorescent lanthanide chelate, for the quantification of human fecal bifidobacteria was evaluated. The specificities of the primers and the primer-probe combination were evaluated by conventional PCR and real-time PCR, respectively. The results obtained by real-time PCR were compared with those obtained by fluorescent in situ hybridization, the current gold standard for intestinal microbiota quantification. In general, a good correlation between the two methods was observed. In order to determine the detection limit and the accuracy of the real-time PCR procedure, germfree rat feces were spiked with known amounts of bifidobacteria and analyzed by both methods. The detection limit of the method used in this study was found to be about 5 × 104 cells per g of feces. Both methods, real-time PCR and fluorescent in situ hybridization, led to an accurate quantification of the spiked samples with high levels of bifidobacteria, but real-time PCR was more accurate for samples with low levels. We conclude that the real-time PCR procedure described here is a specific, accurate, rapid, and easy method for the quantification of bifidobacteria in feces.

scholarly journals P3.03-017 Fluorescent in situ Hybridization Analysis of MET Gene Status in Malignant Mesothelioma

2017 ◽  
Vol 12 (1) ◽  
pp. S1354
Author(s):  
Antonella Vigani ◽  
Sandra Salvi ◽  
Serena Varesano ◽  
Simona Boccardo ◽  
Paola Ferro ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Malignant Mesothelioma ◽  
Fluorescent In Situ Hybridization ◽  
Hybridization Analysis ◽  
Gene Status

scholarly journals Fluorescent In Situ Hybridization 1p/19q Deletion/Imbalance Analysis of Low-Grade and Atypical Meningiomas

2010 ◽  
Vol 50 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Toru NAGASAKA ◽  
Masaharu GUNJI ◽  
Noboru HOSOKAI ◽  
Kumiko HAYASHI ◽  
Masahiko FUJINO ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Low Grade ◽  
Atypical Meningiomas

Isolated cutaneous extramedullary relapse of leukemia confirmed by fluorescent in situ hybridization analysis

2012 ◽  
Vol 67 (4) ◽  
pp. e162-e164 ◽  
Author(s):  
Ben Friedman ◽  
Anokhi Jambusaria ◽  
Michi Shinohara ◽  
Rosalie Elenitsas ◽  
Noelle Frey ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Hybridization Analysis ◽  
Extramedullary Relapse

Quantification of an Eikelboom type 021N bulking event with fluorescence in situ hybridization and real-time PCR

2005 ◽  
Vol 68 (5) ◽  
pp. 695-704 ◽  
Author(s):  
Han Vervaeren ◽  
Kurt De Wilde ◽  
Jorg Matthys ◽  
Nico Boon ◽  
Lutgarde Raskin ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Real Time ◽  
Real Time Pcr
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