Comparative karyotype study of three Cyprinids (Cyprinidae, Cyprininae) in Thailand by classical cytogenetic and FISH techniques

Three species of ornamental fishes in the subfamily Cyprininae (family Cyprinidae) namely, Epalzeorhynchos frenatum (Fowler, 1934), Puntigrus partipentazona (Fowler, 1934), Scaphognathops bandanensis Boonyaratpalin et Srirungroj, 1971 were studied by classical cytogenetic and fluorescent in situ hybridization (FISH) techniques. Chromosomes were directly prepared from kidney tissues and stained by using conventional and Ag-NOR banding techniques. Microsatellite d(CA)15 and d(CGG)10 probes were hybridized to the chromosomes of three cyprinids. The results show that the three cyprinid species share the same diploid number as 2n=50 but there are differences in the fundamental number (NF) and karyotypes i.e. E. frenatum: NF = 78, 18m+10sm+10st+12a; P. partipentazona: NF = 80, 6m+24sm+14st+6a; S. bandanensis: NF = 66, 4m+12sm+34a. NOR positive masks were observed at the regions adjacent to the telomere of the short arm of the chromosome pairs 10 (submetacentric) and 1 (metacentric) in E. frenatum and P. partipentazona, respectively whereas those were revealed at telomeric regions of the long arm of the chromosome pair 9 (acrocentric) in S. bandanensis. The mapping of d(CA)15 and d(CGG)10 microsatellites shown that hybridization signals are abundantly distributed in telomeric regions of several pairs except d(CA)15 repeats in S. bandanensis, which are distributed throughout all chromosomes and d(CGG)10 repeats in P. partipentazona display the high accumulation only in the first chromosome pair.

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Karyotypic variation in the long-whiskered catfish Pimelodus blochii Valenciennes, 1840 (Siluriformes, Pimelodidae) from the lower Tapajós, Amazonas and Trombetas Rivers

Comparative Cytogenetics ◽

10.3897/compcytogen.v12i3.22590 ◽

2018 ◽

Vol 12 (3) ◽

pp. 285-298 ◽

Cited By ~ 1

Author(s):

Ivanny Coelho da Fonseca ◽

Luan Aércio Melo Maciel ◽

Frank Raynner Vasconcelos Ribeiro ◽

Luís Reginaldo Ribeiro Rodrigues

Keyword(s):

Silver Staining ◽

Chromosome Pair ◽

Fluorescent In Situ Hybridization ◽

Species Complex ◽

Diploid Number ◽

Acrocentric Chromosome ◽

5S Rdna ◽

Karyotypic Formula ◽

Karyotypic Variation

The genus Pimelodus LaCépède, 1803 comprises 35 formally recognized species distributed along the major neotropical river basins. Despite conservatism in diploid number with 2n=56, an intense variation of chromosomal morphology (karyotypic formula) has been documented in Pimelodus species. In the present study, we analyzed karyotypes of 20 specimens, identified as Pimelodusblochii Valenciennes, 1840 and collected from the lower courses of the Tapajós, Amazonas and Trombetas Rivers. The karyotypes were characterized by Giemsa conventional staining, C-banding, silver staining (Ag-NOR) and fluorescent in situ hybridization (FISH) with 5S and 18S rDNA probes. The karyotypes showed 2n=56 chromosomes in fish from the Tapajós River. In contrast, fish from the Amazonas and Trombetas Rivers had 2n=58. The nucleolus organizing regions were labeled on the short arm of an acrocentric chromosome as demonstrated by silver staining and FISH. Signals for 18S and 5S rDNA were co-localized on one chromosome pair. Our results demonstrate karyotypic divergence between Tapajós and Amazonas-Trombetas populations of P.blochii, interpreted as supporting the existence of a species complex in this taxon.

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1796: Analysis of Chromosomal Aneuploidy in Patients with Oligoasthenoteratozoospermia(OAT) Using Fluorescent in-situ Hybridization

The Journal of Urology ◽

10.1016/s0022-5347(18)31984-0 ◽

2007 ◽

Vol 177 (4S) ◽

pp. 596-597

Author(s):

Joseph P. Alukal ◽

Bobby B. Najari ◽

Wilson Chuang ◽

Lata Murthy ◽

Monica Lopez-Perdomo ◽

...

Keyword(s):

In Situ Hybridization ◽

Fluorescent In Situ Hybridization ◽

Chromosomal Aneuploidy

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Faculty Opinions recommendation of Clinical experience with preimplantation diagnosis of sex by dual fluorescent in situ hybridization.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.715897919.791352828 ◽

2012 ◽

Author(s):

Magdalena Zernicka-Goetz ◽

Anna Ajduk

Keyword(s):

In Situ Hybridization ◽

Clinical Experience ◽

Fluorescent In Situ Hybridization ◽

Preimplantation Diagnosis

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Two-color fluorescent in situ hybridization in the embryonic zebrafish brain using differential detection systems

BMC Developmental Biology ◽

10.1186/1471-213x-11-43 ◽

2011 ◽

Vol 11 (1) ◽

pp. 43 ◽

Cited By ~ 87

Author(s):

Gilbert Lauter ◽

Iris Söll ◽

Giselbert Hauptmann

Keyword(s):

In Situ Hybridization ◽

Fluorescent In Situ Hybridization ◽

Differential Detection ◽

Zebrafish Brain ◽

Detection Systems

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Fluorescent in situ hybridization (FISH): A useful diagnostic tool for childhood conjunctival melanoma

European Journal of Ophthalmology ◽

10.1177/11206721211030775 ◽

2021 ◽

pp. 112067212110307

Author(s):

Raquel María Moral ◽

Carlos Monteagudo ◽

Javier Muriel ◽

Lucía Moreno ◽

Ana María Peiró

Keyword(s):

In Situ Hybridization ◽

Fluorescent In Situ Hybridization ◽

Pediatric Population ◽

Diagnostic Technique ◽

Histopathological Diagnosis ◽

Fish Analysis ◽

Conjunctival Melanoma ◽

Adequate Treatment ◽

First Case

Introduction: Conjunctival melanoma is extremely rare in children and has low rates of resolution. Definitive histopathological diagnosis based exclusively on microscopic findings is sometimes difficult. Thus, early diagnosis and adequate treatment are essential to improve clinical outcomes. Clinical case: We present the first case in which the fluorescent in situ hybridization (FISH) diagnostic technique was applied to a 10-year-old boy initially suspected of having amelanotic nevi in his right eye. Based on the 65% of tumor cells with 11q13 (CCND1) copy number gain and 33% with 6p25 (RREB1) gain as measured by the FISH analysis, and on supporting histopathological findings, the diagnosis of conjunctival melanoma could be made. Following a larger re-excision, adjuvant therapy with Mitomycin C (MMC), cryotherapy and an amniotic membrane graft, the patient has remained disease-free during 9 years of long-term follow-up. Case discussion: Every ophthalmologist should remember to consider and not forget the possibility of using FISH analyses during the differential diagnosis of any suspicious conjunctival lesions. Genetic techniques, such as FISH, have led to great advances in the classification of ambiguous lesions. Evidence-based guidelines for diagnosing conjunctival melanoma in the pediatric population are needed to determine the most appropriate strategy for this age group.

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Comparison of culture, polymerase chain reaction, and fluorescent in situ hybridization for detection ofBrachyspira hyodysenteriaeand “Brachyspira hampsonii” in pig feces

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638714563064 ◽

2014 ◽

Vol 27 (1) ◽

pp. 41-46 ◽

Cited By ~ 9

Author(s):

Bailey L. Wilberts ◽

Hallie L. Warneke ◽

Leslie P. Bower ◽

Joann M. Kinyon ◽

Eric R. Burrough

Keyword(s):

Polymerase Chain Reaction ◽

In Situ Hybridization ◽

Fluorescent In Situ Hybridization ◽

Chain Reaction ◽

Polymerase Chain

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Combination of Direct Viable Count and Fluorescent In Situ Hybridization (DVC-FISH) as a Potential Method for Identifying Viable Vibrio parahaemolyticus in Oysters and Mussels

Foods ◽

10.3390/foods10071502 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1502

Author(s):

Jorge García-Hernández ◽

Manuel Hernández ◽

Yolanda Moreno

Keyword(s):

In Situ Hybridization ◽

Vibrio Parahaemolyticus ◽

Fluorescent In Situ Hybridization ◽

Potential Method ◽

Viable Count ◽

Hybridization Technique ◽

Fish Technique ◽

Viable Cells

Vibrio parahaemolyticus is a human food-borne pathogen with the ability to enter the food chain. It is able to acquire a viable, non-cultivable state (VBNC), which is not detected by traditional methods. The combination of the direct viable count method and a fluorescent in situ hybridization technique (DVC-FISH) makes it possible to detect microorganisms that can present VBNC forms in complex samples The optimization of the in vitro DVC-FISH technique for V. parahaemolyticus was carried out. The selected antibiotic was ciprofloxacin at a concentration of 0.75 μg/mL with an incubation time in DVC broth of 5 h. The DVC-FISH technique and the traditional plate culture were applied to detect and quantify the viable cells of the affected pathogen in artificially contaminated food matrices at different temperatures. The results obtained showed that low temperatures produced an important logarithmic decrease of V. parahaemolyticus, while at 22 °C, it proliferated rapidly. The DVC-FISH technique proved to be a useful tool for the detection and quantification of V. parahaemolyticus in the two seafood matrices of oysters and mussels. This is the first study in which this technique has been developed to detect viable cells for this microorganism.

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