Statistical Optimization of Milk Clotting Enzyme Biosynthesis by Mucor mucedo Kp736529 and its Further Application in Cheese Production

2015 ◽  
Vol 10 (2) ◽  
pp. 61-76 ◽  
Author(s):  
Ibrahim A.A. Abou Ayana ◽  
Amal Elsady Ibrahim ◽  
Wesam I.A. Saber
Keyword(s):  
Statistical Optimization ◽  
Milk Clotting ◽  
Milk Clotting Enzyme ◽  
Mucor Mucedo ◽  
Cheese Production ◽  
Enzyme Biosynthesis

A novel milk-clotting enzyme from Aspergillus oryzae and A. luchuensis is an aspartic endopeptidase PepE presumed to be a vacuolar enzyme

2022 ◽  
Author(s):  
Yoko Takyu ◽  
Taro Asamura ◽  
Ayako Okamoto ◽  
Hiroshi Maeda ◽  
Michio Takeuchi ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Milk Clotting ◽  
Milk Clotting Enzyme ◽  
Proteolytic Activities ◽  
Homologous Enzyme ◽  
Traditional Fermentation ◽  
Enzyme Source ◽  
Cheese Production ◽  
Milk Clotting Activity ◽  
Aspartic Endopeptidase

Abstract Aspergillus oryzae RIB40 has 11 aspartic endopeptidase genes. We searched for milk-clotting enzymes based on the homology of the deduced amino acid sequence with chymosins. As a result, we identified a milk-clotting enzyme in A. oryzae. We expected other Aspergillus species to have a homologous enzyme with milk-clotting activity, and we found the most homologous aspartic endopeptidase from A. luchuensis had milk-clotting activity. Surprisingly, two enzymes were considered as vacuole enzymes according to a study on A. niger proteases. The two enzymes from A. oryzae and A. luchuensis cleaved a peptide between the 105Phe-106Met bond in κ-casein, similar to chymosin. Although both enzymes showed proteolytic activity using casein as a substrate, the optimum pH values for milk-clotting and proteolytic activities were different. Furthermore, the substrate specificities were highly restricted. Therefore, we expected that the Japanese traditional fermentation agent, koji, could be used as an enzyme source for cheese production.

scholarly journals Technology of obtaining milk-clotting enzyme from fungal culture Funalia Sp. for application in cheese production

2019 ◽  
Author(s):  
Ekaterina Gannochka ◽  
◽  
Boris Kolesnikov ◽  
Alisa Salamahina ◽  
Mark Shamtsyan
Keyword(s):  
Fungal Culture ◽  
Milk Clotting ◽  
Milk Clotting Enzyme ◽  
Cheese Production

Perspectives of using complex milk-clotting enzyme preparations for ripening rennet cheeses production

2019 ◽  
pp. 24-26
Author(s):  
D.V. Abramov ◽  
◽  
D.S. Myagkonosov ◽  
I.N. Delitskaya ◽  
V.A. Mordvinova ◽  
...  
Keyword(s):  
Enzyme Preparations ◽  
Milk Clotting ◽  
Milk Clotting Enzyme

Novel Bacillus Milk-Clotting Enzyme Produces Diverse Functional Peptides in Semihard Cheese

2021 ◽  
Vol 69 (9) ◽  
pp. 2784-2792
Author(s):  
Fanqiang Meng ◽  
Haizhen Zhao ◽  
Fengxia Lu ◽  
Xiaomei Bie ◽  
Zhaoxin Lu ◽  
...  
Keyword(s):  
Milk Clotting ◽  
Milk Clotting Enzyme ◽  
Functional Peptides

Milk-clotting Enzyme from Microorganisms

1971 ◽  
Vol 35 (9) ◽  
pp. 1398-1401
Author(s):  
Juhyun Yu ◽  
Gakuzo Tamura ◽  
Kei Arima
Keyword(s):  
Milk Clotting ◽  
Milk Clotting Enzyme

Milk-clotting Enzyme from Microorganisms

1971 ◽  
Vol 35 (8) ◽  
pp. 1194-1199
Author(s):  
Juhyun Yu ◽  
Gakuzo Tamura ◽  
Kei Arima
Keyword(s):  
Milk Clotting ◽  
Milk Clotting Enzyme
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