An Improved Method for Genomic In situ Hybridization in Oryza Species

2000 ◽  
Vol 3 (10) ◽  
pp. 1601-1604
Author(s):  
Muhammad Asghar ◽  
Darshan S. Brar
Keyword(s):  
In Situ Hybridization ◽  
Genomic In Situ Hybridization ◽  
Oryza Species ◽  
Improved Method

Synthesis and cytological characterization of trigeneric hybrids of durum wheat with and without Ph1

Genome ◽  
2004 ◽  
Vol 47 (6) ◽  
pp. 1173-1181 ◽  
Author(s):  
Prem P Jauhar ◽  
M Doğramaci ◽  
T S Peterson
Keyword(s):  
In Situ Hybridization ◽  
Chromosome Pairing ◽  
Genetic Recombination ◽  
Genomic In Situ Hybridization ◽  
Homoeologous Pairing ◽  
Alien Gene Transfer ◽  
Chromosome 5B ◽  
Alien Gene ◽  
Trigeneric Hybrids

Wild grasses in the tribe Triticeae, some in the primary or secondary gene pool of wheat, are excellent reservoirs of genes for superior agronomic traits, including resistance to various diseases. Thus, the diploid wheatgrasses Thinopyrum bessarabicum (Savul. and Rayss) Á. Löve (2n = 2x = 14; JJ genome) and Lophopyrum elongatum (Host) Á. Löve (2n = 2x = 14; EE genome) are important sources of genes for disease resistance, e.g., Fusarium head blight resistance that may be transferred to wheat. By crossing fertile amphidiploids (2n = 4x = 28; JJEE) developed from F1 hybrids of the 2 diploid species with appropriate genetic stocks of durum wheat, we synthesized trigeneric hybrids (2n = 4x = 28; ABJE) incorporating both the J and E genomes of the grass species with the durum genomes A and B. Trigeneric hybrids with and without the homoeologous-pairing suppressor gene, Ph1, were produced. In the absence of Ph1, the chances of genetic recombination between chromosomes of the 2 useful grass genomes (JE) and those of the durum genomes (AB) would be enhanced. Meiotic chromosome pairing was studied using both conventional staining and fluorescent genomic in situ hybridization (fl-GISH). As expected, the Ph1-intergeneric hybrids showed low chromosome pairing (23.86% of the complement), whereas the trigenerics with ph1b (49.49%) and those with their chromosome 5B replaced by 5D (49.09%) showed much higher pairing. The absence of Ph1 allowed pairing and, hence, genetic recombination between homoeologous chromosomes. Fl-GISH analysis afforded an excellent tool for studying the specificity of chromosome pairing: wheat with grass, wheat with wheat, or grass with grass. In the trigeneric hybrids that lacked chromosome 5B, and hence lacked the Ph1 gene, the wheat–grass pairing was elevated, i.e., 2.6 chiasmata per cell, a welcome feature from the breeding standpoint. Using Langdon 5D(5B) disomic substitution for making trigeneric hybrids should promote homoeologous pairing between durum and grass chromosomes and hence accelerate alien gene transfer into the durum genomes.Key words: alien gene transfer, chiasma (xma) frequency, chromosome pairing, fluorescent genomic in situ hybridization (fl-GISH), homoeologous-pairing regulator, specificity of chromosome pairing, wheatgrass.

Genome constitutions ofRoegneriaalashanica,R.elytrigioides,R.magnicaespesandR.grandis(Poaceae: Triticeae) via genomic in-situ hybridization

2010 ◽  
Vol 28 (2) ◽  
pp. 206-211 ◽  
Author(s):  
Hai-Qing Yu ◽  
Chun Zhang ◽  
Chun-Bang Ding ◽  
Hai-Qin Zhang ◽  
Yong-Hong Zhou
Keyword(s):  
In Situ Hybridization ◽  
Genomic In Situ Hybridization

Genomic in situ hybridization in Brassica amphidiploids and interspecific hybrids

1997 ◽  
Vol 95 (8) ◽  
pp. 1320-1324 ◽  
Author(s):  
R. J. Snowdon ◽  
W. Köhler ◽  
W. Friedt ◽  
A. Köhler
Keyword(s):  
In Situ Hybridization ◽  
Interspecific Hybrids ◽  
Genomic In Situ Hybridization

Analysis of Oriental × Trumpet (OT) Lilium hybrids by genomic in situ hybridization based on ploidy level

2017 ◽  
pp. 253-258
Author(s):  
F. Ramzan ◽  
A. Younis ◽  
K.B. Lim ◽  
S.H. Bae ◽  
M.J. Kwon ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Ploidy Level ◽  
Genomic In Situ Hybridization

A novel genome of C and the first autotetraploid species in the Setaria genus identified by genomic in situ hybridization

2009 ◽  
Vol 56 (6) ◽  
pp. 843-850 ◽  
Author(s):  
Yongqiang Wang ◽  
Hui Zhi ◽  
Wei Li ◽  
Haiquan Li ◽  
Yongfang Wang ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Genomic In Situ Hybridization

Genomic in situ hybridization analysis of a trigenomic hybrid involving Solanum and Lycopersicon species

Genome ◽  
2001 ◽  
Vol 44 (2) ◽  
pp. 299-304 ◽  
Author(s):  
S N Haider Ali ◽  
Dirk Jan Huigen ◽  
M S Ramanna ◽  
Evert Jacobsen ◽  
Richard GF Visser
Keyword(s):  
In Situ Hybridization ◽  
Chromosome Doubling ◽  
Genomic In Situ Hybridization ◽  
Potato Genome ◽  
Meiotic Chromosomes ◽  
Lycopersicon Species ◽  
Bridge Species ◽  
Genomic Probes ◽  
Homoeologous Chromosomes

A 4x potato (+) tomato fusion hybrid (2n = 4x = 48) was successfully backcrossed with a diploid Lycopersicon pennellii (2n = 2x = 24). Genomic in situ hybridization (GISH) on somatic and meiotic chromosomes confirmed that the progenies were triploids (2n = 3x = 36) and possessed three different genomes: potato, tomato, and L. pennellii. Therefore, they have been called trigenomic hybrids. Total genomic probes of both Lycopersicon species were found to hybridize mutually, whereas the potato genome was clearly differentiated. During metaphase I, bivalents were formed predominantly between tomato and L. pennellii chromosomes and the univalents of potato chromosomes were most common. Trivalents in all cases included homoeologous chromosomes of potato, tomato, and L. pennellii. However, the triploids were totally sterile as determined from extensive crossing. On chromosome doubling of triploids by shoot regeneration from callus, hexaploids (2n = 6x = 72) were obtained. Despite exhibiting clear allohexaploid behaviour by forming 36 bivalents at meiosis, these were also completely sterile like their triploid counterparts. In spite of this drawback, the prospects of chromosome pairing between potato L. pennellii and Solanum genomes does open the possibilities for bringing the two genera close.Key words: trigenomic triploids, GISH, bridge species, potato (+) tomato fusion hybrids.

scholarly journals Genomic in situ Hybridization in Triticeae: A Methodological Approach

10.5772/52928 ◽  
2013 ◽  
Author(s):  
Sandra Patussi ◽  
Santelmo Vasconcelos ◽  
Liane Balvedi Poersch ◽  
Ana Rafaela ◽  
Ana Christina
Keyword(s):  
In Situ Hybridization ◽  
Methodological Approach ◽  
Genomic In Situ Hybridization
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