Grafting Long Bone Fractures With Demineralized Bone Matrix Putty Enriched With Bone Marrow: Pilot Findings

Orthopedics ◽  
2006 ◽  
Vol 29 (10) ◽  
pp. 939-941 ◽  
Author(s):  
Ronald W. Lindsey ◽  
Kalia K. Sadasivian ◽  
George W. Wood ◽  
Harrison A. Stubbs ◽  
Jon E. Block
Keyword(s):  
Bone Marrow ◽  
Bone Fractures ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Long Bone ◽  
Long Bone Fractures ◽  
Demineralized Bone

Demineralized bone matrix-based microcarrier scaffold favors vascularized large bone regeneration in vivo in a rat model

2018 ◽  
Vol 33 (2) ◽  
pp. 182-195 ◽  
Author(s):  
Qiannan Li ◽  
Wenjie Zhang ◽  
Guangdong Zhou ◽  
Yilin Cao ◽  
Wei Liu ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Stem Cells ◽  
Bone Marrow ◽  
Bone Regeneration ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Matrix Group ◽  
Micro Computed Tomography ◽  
Demineralized Bone

Insufficient neo-vascularization of in vivo implanted cell-seeded scaffold remains a major bottleneck for clinical translation of engineered bone formation. Demineralized bone matrix is an ideal bone scaffold for bone engineering due to its structural and biochemical components similar to those of native bone. We hypothesized that the microcarrier form of demineralized bone matrix favors ingrowth of vessels and bone regeneration upon in vivo implantation. In this study, a rat model of femoral vessel pedicle-based bone engineering was employed by filling the demineralized bone matrix scaffolds inside a silicone chamber that surrounded the vessel pedicles, and to compare the efficiency of vascularized bone regeneration between microcarrier demineralized bone matrix and block demineralized bone matrix. The results showed that bone marrow stem cells better adhered to microcarrier demineralized bone matrix and produced more extracellular matrices during in vitro culture. After in vivo implantation, microcarrier demineralized bone matrix seeded with bone marrow stem cells formed relatively more bone tissue than block demineralized bone matrix counterpart at three months upon histological examination. Furthermore, micro-computed tomography three-dimensional reconstruction showed that microcarrier demineralized bone matrix group regenerate significantly better and more bone tissues than block demineralized bone matrix both qualitatively and quantitatively (p < 0.05). Moreover, micro-computed tomography reconstructed angiographic images also demonstrated significantly enhanced tissue vascularization in microcarrier demineralized bone matrix group than in block demineralized bone matrix group both qualitatively and quantitatively (p < 0.05). Anti-CD31 immunohistochemical staining of (micro-) vessels and semi-quantitative analysis also evidenced enhanced vascularization of regenerated bone in microcarrier demineralized bone matrix group than in block demineralized bone matrix group (p < 0.05). In conclusion, the microcarrier form of demineralized bone matrix is an ideal bone regenerative scaffold due to its advantages of osteoinductivity and vascular induction, two essentials for in vivo bone regeneration.

The effects of demineralized bone matrix and direct current on an ?in vivo? culture of bone marrow cells

1989 ◽  
Vol 7 (1) ◽  
pp. 22-27 ◽  
Author(s):  
Zachary B. Friedenberg ◽  
Carl T. Brighton ◽  
James D. Michelson ◽  
John Bednar ◽  
Richard Schmidt ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Direct Current ◽  
Bone Marrow Cells ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
In Vivo Culture ◽  
Marrow Cells ◽  
Demineralized Bone

Complications Following Arthroscopic Ankle Arthrodesis

1996 ◽  
Vol 17 (6) ◽  
pp. 340-342 ◽  
Author(s):  
Lynn A. Crosby ◽  
Ted C. Yee ◽  
Teri S. Formanek ◽  
Timothy C. Fitzgibbons
Keyword(s):  
Bone Marrow ◽  
Complication Rate ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Fusion Rate ◽  
Ankle Arthrodesis ◽  
Deep Infection ◽  
Pin Site ◽  
Demineralized Bone

Forty-two patients underwent an arthroscopic ankle arthrodesis utilizing a bi-framed distraction technique and demineralized bone matrix-bone marrow slurry as a graft substitute. The average follow-up was 27 months (range, 12–64 months). The overall complication rate was 55%, including three nonunions (7%), two fractures (4.8%), four pin site infections (9.5%), one deep infection, four hardware problems (9.5%), and four symptomatic painful subtalar joints (9.5%). Overall, 85% of patients were satisfied with their final result. The complication rate was high but most complications were minor and manageable. The demineralized bone matrix and bone marrow did not seem to increase the fusion rate over what has been documented previously for arthroscopic ankle fusions without the use of this graft substitute.

Sign in / Sign up

Export Citation Format

Share Document