The effects of demineralized bone matrix and direct current on an ?in vivo? culture of bone marrow cells

Insufficient neo-vascularization of in vivo implanted cell-seeded scaffold remains a major bottleneck for clinical translation of engineered bone formation. Demineralized bone matrix is an ideal bone scaffold for bone engineering due to its structural and biochemical components similar to those of native bone. We hypothesized that the microcarrier form of demineralized bone matrix favors ingrowth of vessels and bone regeneration upon in vivo implantation. In this study, a rat model of femoral vessel pedicle-based bone engineering was employed by filling the demineralized bone matrix scaffolds inside a silicone chamber that surrounded the vessel pedicles, and to compare the efficiency of vascularized bone regeneration between microcarrier demineralized bone matrix and block demineralized bone matrix. The results showed that bone marrow stem cells better adhered to microcarrier demineralized bone matrix and produced more extracellular matrices during in vitro culture. After in vivo implantation, microcarrier demineralized bone matrix seeded with bone marrow stem cells formed relatively more bone tissue than block demineralized bone matrix counterpart at three months upon histological examination. Furthermore, micro-computed tomography three-dimensional reconstruction showed that microcarrier demineralized bone matrix group regenerate significantly better and more bone tissues than block demineralized bone matrix both qualitatively and quantitatively (p < 0.05). Moreover, micro-computed tomography reconstructed angiographic images also demonstrated significantly enhanced tissue vascularization in microcarrier demineralized bone matrix group than in block demineralized bone matrix group both qualitatively and quantitatively (p < 0.05). Anti-CD31 immunohistochemical staining of (micro-) vessels and semi-quantitative analysis also evidenced enhanced vascularization of regenerated bone in microcarrier demineralized bone matrix group than in block demineralized bone matrix group (p < 0.05). In conclusion, the microcarrier form of demineralized bone matrix is an ideal bone regenerative scaffold due to its advantages of osteoinductivity and vascular induction, two essentials for in vivo bone regeneration.

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74. The influence of various protective agents against freezing damage on the viability of bone marrow cells in an in-Vivo culture

Cryobiology ◽

10.1016/s0011-2240(65)80080-3 ◽

1965 ◽

Vol 2 (1) ◽

pp. 32-33

Keyword(s):

Bone Marrow ◽

Bone Marrow Cells ◽

Freezing Damage ◽

Protective Agents ◽

In Vivo Culture ◽

Marrow Cells

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The Osteogenetic Efficacy of Goat Bone Marrow-Enriched Self-Assembly Peptide/Demineralized Bone Matrix In Vitro and In Vivo

Tissue Engineering Part A ◽

10.1089/ten.tea.2014.0294 ◽

2015 ◽

Vol 21 (7-8) ◽

pp. 1398-1408 ◽

Cited By ~ 6

Author(s):

Zhiqiang Li ◽

Tianyong Hou ◽

Moyuan Deng ◽

Fei Luo ◽

Xuehui Wu ◽

...

Keyword(s):

Bone Marrow ◽

Self Assembly ◽

Demineralized Bone Matrix ◽

Bone Matrix ◽

Demineralized Bone

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In Vitro and In Vivo Study of a Novel Nanoscale Demineralized Bone Matrix Coated PCL/β‐TCP Scaffold for Bone Regeneration

Macromolecular Bioscience ◽

10.1002/mabi.202000336 ◽

2020 ◽

pp. 2000336

Author(s):

Bo Yuan ◽

Zhiwei Wang ◽

Yin Zhao ◽

Yifan Tang ◽

Shengyuan Zhou ◽

...

Keyword(s):

Bone Regeneration ◽

Demineralized Bone Matrix ◽

Bone Matrix ◽

In Vivo Study ◽

Demineralized Bone

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CD34+ human marrow cells that express low levels of Kit protein are enriched for long-term marrow-engrafting cells

Blood ◽

10.1182/blood.v87.10.4136.bloodjournal87104136 ◽

1996 ◽

Vol 87 (10) ◽

pp. 4136-4142 ◽

Cited By ~ 113

Author(s):

I Kawashima ◽

ED Zanjani ◽

G Almaida-Porada ◽

AW Flake ◽

H Zeng ◽

...

Keyword(s):

Bone Marrow ◽

Bone Marrow Cells ◽

Marrow Cell ◽

In Utero ◽

Fetal Sheep ◽

Hematopoietic Stem ◽

Show Evidence ◽

Marrow Cells

Using in utero transplantation into fetal sheep, we examined the capability of human bone marrow CD34+ cells fractionated based on Kit protein expression to provide long-term in vivo engraftment. Twelve hundred to 5,000 CD34+ Kit-, CD34+ Kit(low), and CD34+ Kit(high) cells were injected into a total of 14 preimmune fetal sheep recipients using the amniotic bubble technique. Six fetuses were killed in utero 1.5 months after bone marrow cell transplantation. Two fetuses receiving CD34+ Kit(low) cells showed signs of engraftment according to analysis of CD45+ cells in their bone marrow cells and karyotype studies of the colonies grown in methylcellulose culture. In contrast, two fetuses receiving CD34+ Kit(high) cells and two fetuses receiving CD34+ Kit- cells failed to show evidence of significant engraftment. Two fetuses were absorbed. A total of six fetuses receiving different cell populations were allowed to proceed to term, and the newborn sheep were serially examined for the presence of chimerism. Again, only the two sheep receiving CD34+ Kit(low) cells exhibited signs of engraftment upon serial examination. Earlier in studies of murine hematopoiesis, we have shown stage-specific changes in Kit expression by the progenitors. The studies of human cells reported here are in agreement with observations in mice, and indicate that human hematopoietic stem cells are enriched in the Kit(low) population.

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In Vivo Bone Tissue Formation Induced by Caclium Phosphate Paste Composite with Demineralized Bone Matrix

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.330-332.1091 ◽

2007 ◽

Vol 330-332 ◽

pp. 1091-1094

Author(s):

H. Kim ◽

M. Park ◽

Su Young Lee ◽

Kang Yong Lee ◽

Hyun Min Kim ◽

...

Keyword(s):

Animal Model ◽

Calcium Phosphate ◽

Calcium Phosphate Cement ◽

Demineralized Bone Matrix ◽

Bone Matrix ◽

Marker Gene ◽

Tissue Formation ◽

Bone Tissue Formation ◽

Demineralized Bone

Demineralized bone matrix (DBM)-calcium phosphate cement (CPC) composites were subjected to cellular test of osteogenic potentials and implantation in animal model. The expression of osteogenic marker gene from mouse preosteoblast cell line MC3T3-E1 adhered to the DBM-CPC composite was much higher than plain CPC. In addition, the DBM-CPC composite implanted nude mice revealed osteoinduction between the implanted composite and adjacent tissues, whereas the plain CPC induced osteoconduction.

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