scholarly journals Inhibition of Bursaphelenchus xylophilus Migration at the Nodes of Pinus thunbergii

2010 ◽  
Vol 92 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Eriko Kawaguchi ◽  
Yu Ichihara
Keyword(s):  
Bursaphelenchus Xylophilus ◽  
Pinus Thunbergii

Influence of fungi on multiplication and distribution of the pine wood nematode, Bursaphelenchus xylophilus, in axenized Pinus thunbergii cuttings

Nematology ◽  
2005 ◽  
Vol 7 (6) ◽  
pp. 809-817 ◽  
Author(s):  
Kazuo Suzuki ◽  
Daisuke Sakaue ◽  
Toshihiro Yamada ◽  
Yu Wang
Keyword(s):  
Population Size ◽  
Early Stage ◽  
Bursaphelenchus Xylophilus ◽  
Pinus Thunbergii ◽  
Disease Development ◽  
Pine Wood Nematode ◽  
Pine Wood ◽  
Inoculation Site ◽  
Fungal Hyphae ◽  
Close Relationship

AbstractInfluence of fungi on multiplication and distribution of the pine wood nematode (PWN), Bursaphelenchus xylophilus, was investigated in Pinus thunbergii cuttings. Axenized nematodes and/or one of two fungi isolated from healthy and PWN-killed P. thunbergii were inoculated together into autoclaved cuttings. A close relationship between the existence and distribution of fungal hyphae, and the multiplication and distribution of PWN was observed. The PWN did not multiply when only axenized nematodes were inoculated in the absence of fungi. When fungi were present, PWN population size increased markedly. The number of nematodes was high at sites where fungal hyphae were distributed. It is suggested that the restriction of a large portion of the nematode population near the inoculation site during the early stage of disease development is closely related to restricted distribution of fungal hyphae.

Spatial and temporal distribution of Bursaphelenchus xylophilus inoculated in grafts of a resistant clone of Pinus thunbergii

2019 ◽  
Vol 24 (2) ◽  
pp. 93-99 ◽  
Author(s):  
Gou Nakajima ◽  
Taiichi Iki ◽  
Taro Yamanobe ◽  
Katsunori Nakamura ◽  
Takuya Aikawa
Keyword(s):  
Temporal Distribution ◽  
Bursaphelenchus Xylophilus ◽  
Pinus Thunbergii ◽  
Spatial And Temporal Distribution ◽  
Resistant Clone

scholarly journals Early Symptom Development and Histological Changes Associated with Migration of Bursaphelenchus xylophilus in Seedling Tissues of Pinus thunbergii

Plant Disease ◽  
2000 ◽  
Vol 84 (6) ◽  
pp. 675-680 ◽  
Author(s):  
Y. Ichihara ◽  
K. Fukuda ◽  
K. Suzuki
Keyword(s):  
Cell Death ◽  
Bursaphelenchus Xylophilus ◽  
Pinus Thunbergii ◽  
Pinewood Nematode ◽  
Symptom Development ◽  
Cortical Tissue ◽  
Early Symptom ◽  
Disease Symptoms ◽  
Virulent Isolate ◽  
Resin Canals

In order to clarify the mechanism of pine wilt caused by the pinewood nematode (PWN), Bursaphelenchus xylophilus, nematode migration in tissues and disease symptoms in Pinus thunbergii seedlings were investigated. One-year-old seedlings were inoculated with different pathogenic isolates of PWN under two different temperatures. At an early stage of symptom development, a virulent isolate of PWN multiplied in both bark and xylem and was distributed in cortical resin canals, cortical tissue, and xylem resin canals at 30°C. Cell death and disease symptoms developed in both bark and xylem. The virulent isolate of PWN at 25°C and the avirulent isolate of PWN at 30°C were distributed mainly in cortical resin canals, but rarely in xylem resin canals and cortical tissue. Disease symptoms and cell death occurred in cortical resin canals and rarely occurred in other tissues. These results demonstrated that the virulent isolate of PWN at low temperature and avirulent nematodes could not easily migrate to xylem resin canals and cortical tissue. It was shown that cell death and early symptom development coincided with PWN migration and, therefore, PWN invasion induces cell death and early symptom development.

scholarly journals Virulence of Bursaphelenchus xylophilus Isolated from Naturally Infested Pine Forests to Five Resistant Families of Pinus thunbergii

Plant Disease ◽  
2012 ◽  
Vol 96 (2) ◽  
pp. 249-252 ◽  
Author(s):  
Mitsuteru Akiba ◽  
Makoto Ishihara ◽  
Norio Sahashi ◽  
Katsunori Nakamura ◽  
Mineko Ohira ◽  
...  
Keyword(s):  
Disease Incidence ◽  
Pine Wilt Disease ◽  
Bursaphelenchus Xylophilus ◽  
Pinus Thunbergii ◽  
Breeding Program ◽  
Pinewood Nematode ◽  
Differential Host ◽  
Pine Trees ◽  
Efficient Breeding ◽  
Highly Virulent

Pine wilt disease is one of the most serious epidemic tree diseases in Japan, and resistant pine trees have been developed through a breeding program. To evaluate resistance of resistant families of Japanese black pine, Pinus thunbergii, to the pinewood nematode, Bursaphelenchus xylophilus, isolated from the field, and to determine whether differentiation of pathogenicity to resistant pine families appears in the nematode isolates, seedlings of five resistant pine families were inoculated with 25 nematode isolates. Disease incidence 18 weeks after inoculation was significantly different among nematode isolates and among pine families but there was no interaction effect between nematode isolate and pine family. This indicates that nematode isolates did not have differential host specificity to resistant families of P. thunbergii. Isolate Shimabara, a test isolate of the breeding program, showed the same degree of virulence as the highly virulent isolates frequently used in experiments. However, more virulent isolates than Shimabara were found among the isolates collected from natural pine forest. This indicated that B. xylophilus populations with higher virulence than Shimabara exist in the natural population. These findings are important in development of more efficient breeding procedures for resistant pine trees.

Observations on the embolism and survival of Pinus thunbergii seedlings inoculated with Bursaphelenchus xylophilus

2015 ◽  
Vol 145 (1) ◽  
pp. 227-231 ◽  
Author(s):  
Toshihiro Umebayashi ◽  
Toshihiro Yamada ◽  
Kazunari Fukuhara ◽  
Ryota Endo
Keyword(s):  
Bursaphelenchus Xylophilus ◽  
Pinus Thunbergii
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