Anti-Inflammatory Effect of Chondria crassicaulis Harvey Ethanol Extract in a Mouse Model of Croton Oil-Induced Ear Edema and LPS-Induced RAW 264.7 Cells

Sargassum fulvellum (Turner) C. Agardh has been used to treat various inflammatory diseases, including lump, dropsy, swollen and painful scrotum, and urination problems for several centuries with no side effects. This study aims to investigate the anti-inflammatory effect of the hexane fraction of S. fulvellum (HFS) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and phorbol 12-myristate 13-acetate (PMA)-induced mouse-ear edema. The anti-inflammatory activity of HFS in LPS-stimulated RAW 264.7 cells was investigated by assessing the inhibition of nitric oxide (NO) and pro-inflammatory cytokine production during Griess reaction and enzyme-linked immunosorbent assay (ELISA), respectively. The molecular mechanisms that underlie the anti-inflammatory action of HFS were investigated by analyzing the activation of transcription factor and its upstream signaling proteins. Additionally, an in vivo study of the anti-inflammatory effect of HFS was carried out using PMA-induced mouse-ear edema. HFS inhibited LPS-induced NO production in a dose-dependent manner and suppressed the expression of inducible NO synthase (iNOS) in the RAW 264.7 cells. Further, HFS reduced the production of pro-inflammatory cytokines in the LPS-stimulated RAW 264.7 cells. HFS significantly inhibited LPS-induced nuclear factor kappa B (NF-κB) transcriptional activity and NF-κB translocation into the nucleus by preventing degradation of inhibitor κB-α. Moreover, HFS inhibited the activation of Akt and mitogen-activated protein kinases (MAPKs) in the LPS-stimulated RAW 264.7 cells. Furthermore, HFS suppressed PMA-induced mouse-ear edema. The above data indicate that the anti-inflammatory effects of HFS on LPS-stimulated cells are associated with the suppression of NF-κB through the inhibition of MAPKs and Akt phosphorylation.

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Anti-Inflammatory Effect of Wheat Germ Oil on Lipopolysaccharide-stimulated RAW 264.7 Cells and Mouse Ear Edema

Microbiology and Biotechnology Letters ◽

10.4014/mbl.1601.01001 ◽

2016 ◽

Vol 44 (3) ◽

pp. 236-245 ◽

Cited By ~ 1

Author(s):

Bo-Kyeong Kang ◽

Min-Ji Kim ◽

Da-Hyun Jeong ◽

Koth-Bong-Woo-Ri Kim ◽

Nan-Young Bae ◽

...

Keyword(s):

Wheat Germ ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Wheat Germ Oil ◽

Ear Edema ◽

Mouse Ear ◽

Anti Inflammatory ◽

Mouse Ear Edema ◽

Inflammatory Effect

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Anti-inflammatory Effect of Ethanol Extract from Sargassum fulvellum on Lipopolysaccharide Induced Inflammatory Responses in RAW 264.7 Cells and Mice Ears

Journal of the Korean Society of Food Science and Nutrition ◽

10.3746/jkfn.2014.43.8.1158 ◽

2014 ◽

Vol 43 (8) ◽

pp. 1158-1165 ◽

Cited By ~ 8

Author(s):

Da-Hyun Jeong ◽

Koth-Bong-Woo-Ri Kim ◽

Min-Ji Kim ◽

Bo-Kyeong Kang ◽

Si-Woo Bark ◽

...

Keyword(s):

Inflammatory Responses ◽

Ethanol Extract ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Sargassum Fulvellum ◽

Anti Inflammatory ◽

Inflammatory Effect

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Anti-Inflammatory Comparison of Melatonin and Its Bromobenzoylamide Derivatives in Lipopolysaccharide (LPS)-Induced RAW 264.7 Cells and Croton Oil-Induced Mice Ear Edema

Molecules ◽

10.3390/molecules26144285 ◽

2021 ◽

Vol 26 (14) ◽

pp. 4285

Author(s):

Pimpichaya Sangchart ◽

Panyada Panyatip ◽

Teerasak Damrongrungruang ◽

Aroonsri Priprem ◽

Pramote Mahakunakorn ◽

...

Keyword(s):

Raw 264.7 Cells ◽

Raw 264.7 ◽

Dependent Manner ◽

In Vivo Models ◽

Raw 264.7 Macrophages ◽

Ear Edema ◽

Croton Oil ◽

Anti Inflammatory

The pineal gland is a neuroendocrine organ that plays an important role in anti-inflammation through the hormone melatonin. The anti-inflammatory effects of melatonin and its derivatives have been reported in both in vitro and in vivo models. Our previous study reported the potent antioxidant and neuroprotective activities of bromobenzoylamide substituted melatonin. In silico analysis successfully predicted that melatonin bromobenzoylamid derivatives were protected from metabolism by CYP2A1, which is a key enzyme of the melatonin metabolism process. Therefore, the anti-inflammatory activities of melatonin and its bromobenzoylamide derivatives BBM and EBM were investigated in LPS-induced RAW 264.7 macrophages and croton oil-induced ear edema in mice. The experiments showed that BBM and EBM significantly reduced production of the inflammatory mediators interleukin-6 (IL-6), prostaglandin E2 (PGE2), and nitric oxide (NO) in a dose-dependent manner, but only slightly affected TNF-α in LPS-induced RAW 264.7 macrophages. This suggests that modifying melatonin at either the N1-position or the N-acetyl side chain affected production of NO, PGE2 and IL-6 in in vitro model. In the croton oil-induced mouse ear edema model, BBM, significantly decreased ear edema thickness at 2–4 h. It leads to conclude that bromobenzoylamide derivatives of melatonin may be one of the potential candidates for a new type of anti-inflammatory agent.

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