Anti-Inflammatory Effect of Wheat Germ Oil on Lipopolysaccharide-stimulated RAW 264.7 Cells and Mouse Ear Edema

Sargassum fulvellum (Turner) C. Agardh has been used to treat various inflammatory diseases, including lump, dropsy, swollen and painful scrotum, and urination problems for several centuries with no side effects. This study aims to investigate the anti-inflammatory effect of the hexane fraction of S. fulvellum (HFS) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and phorbol 12-myristate 13-acetate (PMA)-induced mouse-ear edema. The anti-inflammatory activity of HFS in LPS-stimulated RAW 264.7 cells was investigated by assessing the inhibition of nitric oxide (NO) and pro-inflammatory cytokine production during Griess reaction and enzyme-linked immunosorbent assay (ELISA), respectively. The molecular mechanisms that underlie the anti-inflammatory action of HFS were investigated by analyzing the activation of transcription factor and its upstream signaling proteins. Additionally, an in vivo study of the anti-inflammatory effect of HFS was carried out using PMA-induced mouse-ear edema. HFS inhibited LPS-induced NO production in a dose-dependent manner and suppressed the expression of inducible NO synthase (iNOS) in the RAW 264.7 cells. Further, HFS reduced the production of pro-inflammatory cytokines in the LPS-stimulated RAW 264.7 cells. HFS significantly inhibited LPS-induced nuclear factor kappa B (NF-κB) transcriptional activity and NF-κB translocation into the nucleus by preventing degradation of inhibitor κB-α. Moreover, HFS inhibited the activation of Akt and mitogen-activated protein kinases (MAPKs) in the LPS-stimulated RAW 264.7 cells. Furthermore, HFS suppressed PMA-induced mouse-ear edema. The above data indicate that the anti-inflammatory effects of HFS on LPS-stimulated cells are associated with the suppression of NF-κB through the inhibition of MAPKs and Akt phosphorylation.

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Anti-Inflammatory Activity of Dichloromethane Fraction from Katsuwonus pelamis Heart in LPS-Induced RAW 264.7 Cells and Mouse Ear Edema

Microbiology and Biotechnology Letters ◽

10.4014/mbl.1609.09008 ◽

2017 ◽

Vol 45 (2) ◽

pp. 101-109 ◽

Cited By ~ 1

Author(s):

Min-Ji Kim ◽

Nan-Young Bae ◽

Hyeun-Deok Choi ◽

Koth-Bong-Woo-Ri Kim ◽

Sun-Hee Park ◽

...

Keyword(s):

Inflammatory Activity ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Katsuwonus Pelamis ◽

Ear Edema ◽

Mouse Ear ◽

Anti Inflammatory ◽

Mouse Ear Edema

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Antifungal, Anti-Inflammatory and Cytotoxic Effects of Zingiber cassumunar Gel

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.773.360 ◽

2018 ◽

Vol 773 ◽

pp. 360-364 ◽

Cited By ~ 1

Author(s):

Sroisiri Thaweboon ◽

Boonyanit Thaweboon ◽

Rattiporn Kaypetch

Keyword(s):

Cell Culture ◽

Antifungal Activity ◽

Diffusion Method ◽

Cytotoxic Effects ◽

Ear Edema ◽

Mouse Ear ◽

Anti Inflammatory ◽

Zingiber Cassumunar ◽

Mouse Ear Edema ◽

Inflammatory Effect

This study aimed to investigate the antifungal, anti-inflammatory and cytotoxic effects of Zingiber cassumunar gel. The gel was prepared from essential oil of Zingiber cassumunar rhizome by the Thailand Institute of Scientific and Technological Research. Antifungal activity of the gel was firstly determined by the well diffusion method against Candida albicans ATCC 10238 and candida strain isolated from the patient’s lesion. Then, the Agar overlay technique was used to test the cytotoxicity of Z. cassumunar gel on mouse fibroblasts (ATCC clone 929) according to ISO 7405. For anti-inflammatory effect of the gel, TPA (carrageenan lambda type IV, 12-O-tetradecanoylphorbol-13- acetate)-induced mouse ear edema method was used. The results of well diffusion showed that Z. cassumunar gel was quite a potent antifungal agent against both strains of tested C. albicans with inhibition zones of 12-13 mm. In the cytotoxicity test, the gel exhibited no toxicity to cell culture. In addition, topical administration of Z. cassumunar gel could decrease mouse ear edema induced by TPA. At 30 and 60 min-time points, Z. cassumunar gel showed higher anti-inflammatory activity than triamcinolone which was used as reference anti-inflammatory drug. In conclusion, gel prepared from Z. cassumunar oil showed antifungal activity against both strains of C. albicans. In addition, its anti-inflammatory effect was demonstrated within 30 min by the TPA-induced mouse ear edema model. The gel was non-toxic to cell culture after 24-h incubation. Further studies are needed to clarify the safety and benefit of this gel for clinical use in the treatment of candidal infection and inflammation.

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Anti-Inflammatory Effect of Sargassum patens C. Agardh Ethanol Extract in LPS-induced RAW264.7 Cells and Mouse Ear Edema

Microbiology and Biotechnology Letters ◽

10.4014/mbl.1610.10002 ◽

2017 ◽

Vol 45 (2) ◽

pp. 110-117 ◽

Cited By ~ 2

Author(s):

Min-Ji Kim ◽

Min-Ju Kim ◽

Koth Bong Woo Ri Kim ◽

Sun Hee Park ◽

Hyeun Deok Choi ◽

...

Keyword(s):

Ethanol Extract ◽

Raw264.7 Cells ◽

Ear Edema ◽

Mouse Ear ◽

Anti Inflammatory ◽

Sargassum Patens ◽

Mouse Ear Edema ◽

Inflammatory Effect

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Retrofractamide C Derived from Piper longum Alleviates Xylene-Induced Mouse Ear Edema and Inhibits Phosphorylation of ERK and NF-κB in LPS-Induced J774A.1

Molecules ◽

10.3390/molecules25184058 ◽

2020 ◽

Vol 25 (18) ◽

pp. 4058 ◽

Cited By ~ 1

Author(s):

Hyung Jin Lim ◽

Seon Gyeong Bak ◽

Eun Jae Park ◽

Sae-Kwang Ku ◽

Soyoung Lee ◽

...

Keyword(s):

Biological Activities ◽

Edema Formation ◽

Ear Edema ◽

Extracellular Signal ◽

Edema Model ◽

Mouse Ear ◽

Anti Inflammatory ◽

Factor Α ◽

Mouse Ear Edema ◽

Inflammatory Effect

Many studies have reported the biological activities of retrofractamide C (RAC). However, few studies have investigated the anti-inflammatory effect of RAC. In the present study, we investigated the anti-inflammatory effect of RAC using lipopolysaccharide (LPS)-induced J774A.1 cells and a xylene-induced mouse ear edema model. Treatment with RAC decreased LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) secretion and inducible NO synthase (iNOS) and cyclooxygenase 2 (COX2) protein expression. It also downregulated the LPS-induced production of interleukin-1β (IL-1β) and interleukin-6 (IL-6) but not tumor necrosis factor α (TNF-α). In the LPS-induced signaling pathway, RAC inhibited the phosphorylation of extracellular signal-regulated kinase (ERK) and nuclear factor kappa light chain enhancer of activated B cells (NF-κB) but not c-Jun N-terminal kinase (JNK) or p38. In a xylene-induced mouse ear edema model, RAC treatment alleviated edema formation and inflammatory cell infiltration. In conclusion, the present study indicates that RAC has the potential to have anti-inflammatory effects and could be a prospective functional food.

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