Surface Modified Magnetic Silica Particles for the Immobilization of Laccase and its Use for Pentachlorophenol Removal in Water

2012 ◽  
Vol 178-181 ◽  
pp. 657-660
Author(s):  
Ying Wang ◽  
Di Zhang ◽  
Xue Li ◽  
Bo Li

Magnetic Cu2+-chelated silica particles using polyacrylamide (PAM) as a metal-chelating ligand were developed and used for the immobilization of laccase by coordination. The particles were characterized by (XRD). The effect of Cu2+ and PAM concentrations on the enzymatic activity and the catalytic capacity for pentachlorophenol (PCP) degradation were evaluated. The results showed that the optimum Cu2+ and PAM concentrations were 0.2 mol/L and 2 g/L, respectively. The immobilized laccase prepared in this work exhibited a good catalytic capacity for PCP removal from aqueous solutions.

2014 ◽  
Vol 670-671 ◽  
pp. 267-270 ◽  
Author(s):  
Ying Liu ◽  
Zhen Wang

Surface modified magnetic silica particles for the immobilization of laccase. The maximal absorption capacities of laccase were evaluated systemically. Compared with unmodified magnetic silica particles, the modified magnetic silica particles showed better absorption capacity of the laccase. The immobilized laccase has been used to treat pulp and paper making wastewater, and showed high efficacy on the removal of COD.


Langmuir ◽  
1994 ◽  
Vol 10 (1) ◽  
pp. 92-99 ◽  
Author(s):  
Albert P. Philipse ◽  
Michel P. B. van Bruggen ◽  
Chellapah Pathmamanoharan

TAPPI Journal ◽  
2017 ◽  
Vol 16 (10) ◽  
pp. 559-564 ◽  
Author(s):  
ZHEN WANG ◽  
PINGPING BIN ◽  
YING LIU ◽  
YU LIU ◽  
GUIHUA YANG ◽  
...  

Epoxidized dialdehyde cellulose (EDC) was prepared and grafted with melamine to obtain melamine grafted epoxidized dialdehyde cellulose (EDC-melamine); the products were characterized by various methods and were used as carriers to immobilize laccase. Results show EDC-melamine can immobilize laccase effectively and have higher enzymatic activity compared with EDC. Furthermore, the enzymatic activity of EDC-melamine was found to be as high as 865 U•mg-1, compared with 140U•mg-1 for EDC. The removal efficiency of 2,4-dichlorophenol (2,4-DCP) for EDC-melamine immobilized laccase was about 71.5% at 40°C for 4 h at 10.0 mg•L-1 and dosage of laccase = 0.2 g/L. The removal efficiency can remain greater than 63%, even after six cycles.


2013 ◽  
Vol 71 (04) ◽  
pp. 602 ◽  
Author(s):  
Jianbo Zhang ◽  
Yuxiang Yang ◽  
Meijuan Tian ◽  
Changjian Deng ◽  
Lei Cao ◽  
...  

2000 ◽  
Vol 14 (14) ◽  
pp. 1813-1827 ◽  
Author(s):  
Zhenghe Xu ◽  
Ruan Chi ◽  
T. Difeo ◽  
J. A. Finch

Nanoscale ◽  
2016 ◽  
Vol 8 (5) ◽  
pp. 3019-3030 ◽  
Author(s):  
Diego Estupiñán ◽  
Markus B. Bannwarth ◽  
Steven E. Mylon ◽  
Katharina Landfester ◽  
Rafael Muñoz-Espí ◽  
...  

Multiple functionalization of magnetic silica particles allows simultaneously their bio-orthogonal modification and less unspecific protein adsorption to occur.


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