Protein Adsorption
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Yujie Wang ◽  
Boce Zhang ◽  
Hanna Dodiuk ◽  
Shmuel Kenig ◽  
Carol Barry ◽  

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7079
Michael Assfalg

Protein adsorption onto surfaces of diverse materials of both natural and artificial origin is of utmost relevance in many areas of research and technology: medicine, pharmaceutical sciences, analytical sciences, biotechnology, nanotechnology, and cell biology, among others [...]

2021 ◽  
Vol 11 (1) ◽  
Apurba Das ◽  
Pamu Dobbidi ◽  
Aman Bhardwaj ◽  
Varun Saxena ◽  
Lalit M. Pandey

AbstractThe article investigates electrically active ceramic composite of $$\mathrm{Ca}_{10}(\mathrm{PO}_4)_6(\mathrm{OH})_2$$ Ca 10 ( PO 4 ) 6 ( OH ) 2 (HAP) and $$\mathrm{Ba}_{0.5}\mathrm{Sr}_{0.5}\mathrm{TiO}_{3}$$ Ba 0.5 Sr 0.5 TiO 3 (BST) for biomedical applications. The study is a systematic blend of the materials science aspect of composites with a special focus on the dielectric and biological properties and their relationships. The article emphasized primarily extracting the dielectric constant ($$\epsilon _r)$$ ϵ r ) of the specimens (that lay in the range of 3–65) and related them to microstructural properties like the grain size and at.% of BST. A broad outlook on the importance of $$\epsilon _r$$ ϵ r in determining the suitability of bioceramics for clinical applications is presented. Bioactivity analysis of the specimens led to probing the surface charges (that were negative), and it was found crucial to the growth of dense apatite layers. Furthermore, the cytocompatibility of the specimens displayed cell viability above 100% for Day 1, which increased substantially for Day 3. To reveal other biological properties of the composites, protein adsorption studies using bovine serum albumin (BSA) and fetal bovine serum (FBS) was carried out. Electrostatic interactions govern the adsorption, and the mathematical dependence on surface charges is linear. The protein adsorption is also linearly correlated with the $$\epsilon _r$$ ϵ r , intrinsic to the biomaterials. We delve deeper into protein–biomaterials interactions by considering the evolution of the secondary structure of BSA adsorbed into the specimens. Based on the investigations, 20 at.% HAP–80 at.% BST (20H–80B) was established as a suitable composite comprising the desired features of HAP and BST. Such explorations of electrical and biological properties are interesting for modulating the behavior of bioceramic composites. The results project the suitability of 20H–80B for designing electrically active smart scaffolds for the proposed biomedical applications and are expected to incite further clinical trials.

Materials ◽  
2021 ◽  
Vol 14 (22) ◽  
pp. 6793
Xiaofei Yan ◽  
Chenkai Zhu ◽  
Ju Huang ◽  
Dongmin Qi ◽  
Jiawei Li

The growth and reproduction of microorganisms on fabrics could not only affect the wearability of textiles but also cause harm to human health, and it is an important problem that should be solved to reduce the adsorption and growth of microorganisms on the surface of the fabric. A series of ω-vinyl betaine copolymers were synthesized by catalytic chain transfer polymerization (CCTP) and were modified by mercapto-vinyl click chemistry to synthesize silane-modified betaine copolymers, which were used to treat the cotton fabric. The hydrophilic–hydrophobic transition performance and anti-protein specific adhesion performance of cotton fabric with the betaine copolymer were systematically investigated. The copolymer was confirmed to be successfully finished on the cotton fabric via 1H–NMR and FTIR. The cotton fabric, which was treated by the betaine copolymer, presented temperature response performance in the range of 30–55 °C and had excellent anti-protein adsorption performance. The treated fabric had the best temperature-sensitive and anti-protein specific absorption performance among all the specimens when the mass fraction of G06B in DMAPS was 6 wt.%.

2021 ◽  
pp. 110892
Abdalla Abdal-hay ◽  
Faheem A.Sheikh ◽  
N. Gómez-Cerezo ◽  
Abdulrahman Alneairi ◽  
Monis Luqman ◽  

2021 ◽  
pp. 151779
Lidan Zhao ◽  
Tianqing Liu ◽  
Xiangqin Li ◽  
Qianqian Cui ◽  
Xin Wang ◽  

2021 ◽  
Vol 11 (10) ◽  
pp. 1628-1635
Li Dai ◽  
Xiaohui Yu ◽  
Chusheng Miao ◽  
Shefeng Zheng ◽  
Shanshan Li ◽  

Apoptosis signal-regulating kinase 1 (ASK1) signaling is involved in the occurrence and progression of diabetic nephropathy (DN) renal fibrosis, yet the detection of ASK1 requires further investigation. To explore the effect of magnetic nanomaterials combined with ASK1 signaling on renal fibrosis, a DN rat model was generated. Thirty Wistar rats were randomly allocated into three experimental models: control, sham-operated, and DN model groups. Concentrations of postoperative serum creatinine (Scr) and blood urea nitrogen (BUN) were measured to determine the success of the DN model group. Chitosan coated magnetic hydrophilic nanomaterials ([email protected] MCNCs) were prepared by a “one-pot reaction” method, and further characterized to assess their protein adsorption properties. ASK1 protein expression was analyzed by combining conventional immunohistochemistry and Western blotting techniques with the magnetic nanomaterials. Serum Scr and BUN levels in the DN model group were significantly increased (P < 0.01), indicating that the model was successfully generated. [email protected] MCNCs were characterized and demonstrated effective hydrophilicity, magnetism, and protein adsorption capacity, highlighting its potential application in proteomic research. The detection of ASK1 protein expression was consistent with that of immunohistochemistry and Western blotting when using the magnetic nanomaterials. Furthermore, ASK1 protein expression in the DN model group was significantly increased compared with the control and sham-operated groups. In this study, magnetic hydrophilic nanomaterials were demonstrated to be feasible for protein detection. In addition, the results indicated the potential involvement of ASK1 signaling in the process of renal fibrosis in DN rats.

2021 ◽  
pp. 101565
Ana P.F. Monteiro ◽  
Gaelle Idczak ◽  
Rémi G. Tilkin ◽  
Romain M. Vandeberg ◽  
Bénédicte Vertruyen ◽  

Katharina Gegenschatz-Schmid ◽  
Stefano Buzzi ◽  
Jonas Grossmann ◽  
Bernd Roschitzki ◽  
Riccardo Urbanet ◽  

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