The Temperature Control System Design of Microchip Level PCR Instrument Based on SOPC

A design approach of the temperature control of a novel microchip level PCR instrument based on SOPC technique is presented in this paper. The soft-core processor with RISC framework, NIOS II, which embedded in the FPGA (CyclonIII EP3C16F484C6) of Altera corp., is used as the key component to control the PWM controller achieving the temperature control of the micro-chamber stationary microchip level PCR instrument, to control the Avalon stream slave peripheral acquiring controller achieving the temperature signal detection by the flexible snake like copper wire temperature sensor, and to control the micro-pump and the micro-valve achieving the sample injection and sample outflow controlling. Simultaneously, the control temperature algorithm based on the Smith predictive and adaptive Fuzzy-PID is adapted in this system. The design of PCR microchip, the Smith predictive adaptive Fuzzy-PID temperature control algorithm, the hardware framework and the software design of control system are mainly introduced. And the simulation results testified the correctness of the design method and practicability. Keywords: Microchip level PCR instrument; Smith predictive and adaptive Fuzzy-PID controller; SOPC; The flexible snake like copper wire temperature sensor