scholarly journals Comparison of Nested Polymerase Chain Reaction and Real-Time Polymerase Chain Reaction Targeting 47kDa Gene for the Diagnosis of Scrub Typhus

2019 ◽  
Vol 37 (1) ◽  
pp. 50-53
Author(s):  
Seethalakshmi Srinivasan ◽  
Saravanan Kalaimani ◽  
John Antony Jude Prakash ◽  
Thangam Menon
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Scrub Typhus ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of Bovine Leukemia Virus in Blood and Milk by Nested and Real-Time Polymerase Chain Reactions

2003 ◽  
Vol 15 (1) ◽  
pp. 72-76 ◽  
Author(s):  
Christopher J. Kuckleburg ◽  
Christopher C. Chase ◽  
Eric A. Nelson ◽  
Salvatore A. E. Marras ◽  
Matthew A. Dammen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Early Detection ◽  
Real Time ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Polymerase Chain

Concerns about retroviruses in livestock and products derived from them have necessitated the development of tests to detect the bovine leukemia virus (BLV) in blood and milk from cattle. Dairy cattle ( n = 101) from 5 different geographical areas were used for this study. A nested polymerase chain reaction (PCR) identified 98% of BLV seropositive cattle ( n = 80) from blood and 65% from milk, whereas real-time PCR detected 94% of BLV seropositive cattle from blood and 59% from milk. Bovine leukemia virus was also detected by PCR in approximately 10% of seronegative cattle ( n = 21), most likely because of early detection before seroconversion.

Detection of scrub typhus by real-time polymerase chain reaction and immunoglobulin M ELISA among patients with acute febrile illness

2020 ◽  
Vol 11 (1) ◽  
pp. 66
Author(s):  
Sathish Sankar ◽  
Nithiyanandan Saravanan ◽  
Prashanth Rajendiran ◽  
Mageshbabu Ramamurthy ◽  
Archana Sasimohan ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Scrub Typhus ◽  
Febrile Illness ◽  
Immunoglobulin M ◽  
Acute Febrile Illness ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Validation of Geno-Sen's scrub typhus real time polymerase chain reaction kit by its comparison with a serological ELISA Test

2017 ◽  
Vol 9 (3) ◽  
pp. 108
Author(s):  
Selvaraj Stephen ◽  
Velmurugan Anitharaj ◽  
Jothimani Pradeep ◽  
Pratheesh Pooja ◽  
Sridharan Preethi
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Scrub Typhus ◽  
Elisa Test ◽  
Chain Reaction ◽  
Polymerase Chain
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