Genetic analysis and molecular markers associated with multi-gynoecia (Mg) gene in Trigrain whea

2009 ◽  
Vol 89 (5) ◽  
pp. 845-850 ◽  
Author(s):  
Z Wang ◽  
D Xu ◽  
J Ji ◽  
J Wang ◽  
M Wang ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Simple Sequence Repeats ◽  
Segregation Analysis ◽  
Dominant Gene ◽  
Distal Region ◽  
Allelism Test ◽  
Single Dominant Gene ◽  
Gene Segregation ◽  
Genetic Patterns ◽  
Simple Sequence

Trigrain wheat normally produces up to three gynoecia in a single floret and forms three close-set grains. The gene conferring the multi grain phenotype was earlier designated Mg, the multiple gynoecia gene. Different genetic patterns controlling this trait have been reported. In the present work we studied the inheritance of the three grains trait and identified simple sequence repeats (SSR) markers linked to the Mg gene. Segregation analysis in the cross IGDB-TW (trigrain wheat)/Chinese Spring confirmed that a single dominant gene controlled the three grains trait. An allelism test showed that the same gene controlled the trigrain trait in line Trigrain-Yin 1. A total of 339 microsatellite markers were tested for polymorphism by bulked segregation analysis (BSA) in an F2 population. Six microsatellite markers, Xcfd233, Xgdm6, Xgdm87, Xgwm311, Xgwm349 and Xgwm539, on chromosome 2DL, were linked to Mg. Using the CS 2D deletion lines, Mg gene was localized to the distal region of chromosome 2DL. The microsatellite markers identified in this study have the potential for further mapping and map-based cloning of the gene.Key words: Simple sequence repeats, physical mapping, trigrain wheat

The identification of random amplified polymorphic DNA markers for daylength insensitivity in oat

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 910-914 ◽  
Author(s):  
Charlene P. Wight ◽  
Greg A. Penner ◽  
Louise S. O'Donoughue ◽  
Vernon D. Burrows ◽  
Stephen J. Molnar ◽  
...  
Keyword(s):  
Avena Sativa ◽  
Dna Markers ◽  
Field Data ◽  
Bulk Segregant Analysis ◽  
Segregation Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Dominant Gene ◽  
Dominant Allele ◽  
Single Dominant Gene ◽  
Polymorphic Bands

Daylength insensitive accessions of Avena sativa L. are being used to develop cultivars that will flower normally when grown under short or long photoperiods. Field data indicate that the insensitivity trait is under the control of a single dominant gene, designated Di1. The random amplified polymorphic DNA (RAPD) technique and bulk segregant analysis of daylength sensitive and insensitive plants were used to find markers for this gene. Five of 200 random decamer primers tested produced polymorphic bands, which were shown to be linked to the trait using 30 homozygous insensitive and 30 homozygous sensitive F3 individuals. Three of the markers produced a band in the presence of the dominant allele, and two in its absence. Segregation analysis showed that markers 221 and 136 could be mapped to within 9.8 ± 4.6 and 13.9 ± 5.4 cM of the trait, respectively; that is, close enough to be useful in a breeding program. A study of different cultivars suggested that the band produced by primer 136 is actually the more closely linked marker and the only one present in the original Di1 gene donor CAV2700. The possibility of using both markers in populations derived from different cultivars is discussed.Key words: RAPD, molecular marker, oat, daylength insensitivity, photoperiod, bulk segregant analysis.

Survey and analysis of simple sequence repeats in the Laccaria bicolor genome, with development of microsatellite markers

2010 ◽  
Vol 57 (2) ◽  
pp. 75-88 ◽  
Author(s):  
Jessy Labbé ◽  
Claude Murat ◽  
Emmanuelle Morin ◽  
François Le Tacon ◽  
Francis Martin
Keyword(s):  
Microsatellite Markers ◽  
Simple Sequence Repeats ◽  
Laccaria Bicolor ◽  
Simple Sequence

scholarly journals Mapping of resistance genes to races 1, 3 and 5 of Podosphaera xanthii in melon PI 414723

2013 ◽  
Vol 13 (4) ◽  
pp. 349-355 ◽  
Author(s):  
Ana Carolina Fazza ◽  
Leandro José Dallagnol ◽  
Ana Cristina Fazza ◽  
Carolina C. Monteiro ◽  
Bruno Marco de Lima ◽  
...  
Keyword(s):  
Linkage Group ◽  
Genetic Map ◽  
Resistance Genes ◽  
Segregation Analysis ◽  
Specific Resistance ◽  
Dominant Gene ◽  
Inheritance Of Resistance ◽  
Podosphaera Xanthii ◽  
Single Dominant Gene ◽  
First Time

The fungus Podosphaera xanthii affects melon crops and presents several races controlled by race-specific resistance genes. The accession PI 414723 is resistant to races 1, 3 and 5 and it is a suitable source of resistance genes. The inheritance of resistance to these races was analyzed on 87 F2 plants from the cross of PI 414723 × Védrantais, and resistance to all three races could be explained by the segregation of a single dominant gene, although a digenic model could also be accepted. A genetic map was assembled with 206 markers, and co-segregation analysis of resistance phenotypes indicated the existence of two linked loci in linkage group II, one conferring resistance to races 1 and 5 (denominated Pm-x1,5), and the second to race 3 (denominated Pm-x3), located 5.1 cM apart. This study reports for the first time the existence of Pm-x3 and the genetic locations of these resistance genes from PI 414723.

Survey and analysis of simple sequence repeats in the Ustilaginoidea virens genome and the development of microsatellite markers

Gene ◽  
2016 ◽  
Vol 585 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Mina Yu ◽  
Junjie Yu ◽  
Huanhuan Li ◽  
Yahui Wang ◽  
Xiaole Yin ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Simple Sequence Repeats ◽  
Ustilaginoidea Virens ◽  
Simple Sequence

scholarly journals Tagging microsatellite marker to a blast resistance gene in the irrigated rice cultivar Cica-8

2012 ◽  
Vol 12 (3) ◽  
pp. 164-170 ◽  
Author(s):  
Thiago Martins Pinheiro ◽  
Leila Garcês de Araújo ◽  
Valácia Leme da Silva-Lobo ◽  
Anne Sitarama Prabhu ◽  
Marta Cristina de Filippi
Keyword(s):  
Microsatellite Markers ◽  
Blast Resistance ◽  
Dominant Gene ◽  
Rice Cultivar ◽  
Single Dominant Gene ◽  
Leaf Blast ◽  
Irrigated Rice ◽  
Blast Resistance Gene ◽  
Differential Reaction ◽  
Greenhouse Tests

The rice cultivar Cica-8 exhibit differential reaction to several pathotypes of Magnaporthe oryzae. The objective of the present investigation was to determine the number of alleles involved in the expression of resistance to leaf blast and identify microsatellite markers linked to these alleles. A cross between cultivar Metica-1 and Cica-8 susceptible and resistant, respectively, to pathotype IB-1 (Py1049) was made to obtain F1, F2, BC1:1 and BC1:2 progenies. Greenhouse tests for leaf blast reaction showed that resistance is controlled by a monogenic dominant gene. For testing microsatellite markers, DNA of both resistant and susceptible parents and F1 and F2 populations was extracted. As expected for single dominant gene the F2 populations segregated at a ratio of 3:1. Of the 11 microsatellite markers tested, one marker RM 7102 was found to be closely linked to the resistant allele at a distance of 2.7 cM, in the cultivar Cica-8 to pathotype IB-1.

Polymorphism revealed by simple sequence repeats

1996 ◽  
Vol 1 (7) ◽  
pp. 215-222 ◽  
Author(s):  
W POWELL ◽  
G MACHRAY ◽  
J PROVAN
Keyword(s):  
Simple Sequence Repeats ◽  
Simple Sequence

Resistance to Lettuce Infectious Yellows Virus in Melon

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 534b-534
Author(s):  
James D. McCreight
Keyword(s):  
Cucumis Melo ◽  
Dominant Gene ◽  
Field Tests ◽  
Single Dominant Gene ◽  
Limited Data ◽  
Sweetpotato Whitefly ◽  
Sources Of Resistance ◽  
Lettuce Infectious Yellows Virus ◽  
Greenhouse Tests

Yellowing of melon (Cucumis melo L.) incited by lettuce infectious yellows virus (LIYV) reduces yield and fruit quality of infected plants. LIYV is transmitted only by the sweetpotato whitefly (Bemisia tabaci Genn.). Two naturally infected field tests indicated several potential sources of resistance to LIYV. PI 124112 and `Snake Melon' had mild symptoms in both field tests whereas PI 313970 was asymptomatic in the test in which it was included. In greenhouse tests using controlled inoculation, PI 313970 was asymptomatic, had negative ELISA assays for LIYV, and was negative for LIYV in serial transfers to Chenopodium. `Top Mark' and `PMR 5' were symptomatic, had positive ELISA assays for LIYV, and were positive for LIYV in serial transfers to Chenopodium in these greenhouse tests. Limited data indicate that resistance in PI 313970 is conditioned by a single, dominant gene.

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